Accession ID: MIRT005504 [miRNA, hsa-miR-10b-5p :: NF1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-10bLinkOut: [miRBase ]
Synonyms MIRN10B, hsa-mir-10b, miRNA10B, mir-10b, MIR10B
Description Homo sapiens miR-10b stem-loop
Comment This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-10b-5p
Mature Sequence 27| UACCCUGUAGAACCGAAUUUGUG |49
Evidence Experimental
Experiments Cloned
Putative hsa-miR-10b-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol NF1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms NFNS, VRNF, WSS
Description neurofibromin 1
Transcript NM_0002    LinkOut: [ RefSeq ]
Other Transcripts NM_0010424 , NM_0011281   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on NF1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of NF1
(miRNA target sites are highlighted)
>NF1|NM_0002|3'UTR
   1 AGCTTGCTTGCTTTCTTTTTTAAAATCAACTTAACATGGGCTCTTCACTAGTGACCCCTTCCCTGTCCTTGCCCTTTCCC
  81 CCCATGTTGTAATGCTGCACTTCCTGTTTTATAATGAACCCATCCGGTTTGCCATGTTGCCAGATGATCAACTCTTCGAA
 161 GCCTTGCCTAAATTTAATGCTGCCTTTTCTTTAACTTTTTTTCTTCTACTTTTGGCGTGTATCTGGTATATGTAAGTGTT
 241 CAGAACAACTGCAAAGAAAGTGGGAGGTCAGGAAACTTTTAACTGAGAAATCTCAATTGTAAGAGAGGATGAATTCTTGA
 321 ATACTGCTACTACTGGCCAGTGATGAAAGCCATTTGCACAGAGCTCTGCCTTCTGTGGTTTTCCCTTCTTCATCCTACAG
 401 AGTAAAGTGTTAGTCCTATTTATACATTTTTCAAGATACAAGTTTATGAGAGAAATAGTATTATAACCCCAGTATGTTTA
 481 ATCTTTTAGCTGTGGACTTTTTTTTTAACCGTACAAAACTGAAAGAACCATAGAGGTCAAGCCTCAGTGACTTGACACCA
 561 TAAAGCCACAGACAAGGTACTTGGGGGGGAGGGCAGGGAAATTTCATATTTTATAGTGGATTCTTAAGAAATACTAACAC
 641 TTGAGTATTAGCAATAATTACAGGAAAATAAGTGCGACCACATATATCTTAACATTACTGAATTAAAACTATGGCTTCTA
 721 AGTCCTTATCCAAACTCAGTCATCCAAACTAGTTTATTTTTTTCTCCAGTTGATTATCTTTTAATTTTTAATTTTGCTAA
 801 AGGTGGTTTTTTTGTGTTTTGTTTTTTGTAAACCAAAACTATACTAAGTATAGTAATTATATATATATATATATTTTTTC
 881 CCCTCCCCCTCTTCTTTCCTAACTAATTCTGAGCAGGGTAATCAGTGAACAAAGTGTTGAAAATTGTTCCCAGAAGGTAA
 961 TTTTCATAGATGTTTGCATTAGCTCCATAGCAAAATGGAATGGTACGTGACATTTAGGGTAGCTGATATTTTTATTTTGT
1041 TAAATAATTTCCAAGAATAGAGTATGGTGTATATTATAAATTTCTTTGATAAGATGTATTTTGAATGTCTTTTAATCTTC
1121 CTCCTCCTCTCCAAAAAAATCAGAAACCTCTTTAAGAAAACATGTAGGTTATATATGCTAGAATTGCATTTAATCACTGT
1201 GAAAAGACTGGTCAGCCTGCATTAGTATGACAGTAGGGGGGCTGTTAGAATTGCTGCTATACTGGTGGTATGGATTATCA
1281 TGGCATTGGAATTTTCATAGTAATGCAGATCCAATTTCTTTGTGGTACCTGCAGTTTACAAAATAATTTGACTTCAGTGA
1361 GCATATTGGTATCTGGATGTTCCAATTTAGAACTAAACCATATTTATTACAAAAAGATATTAATCCCTCTACTCCCAGGT
1441 TCCCTTTATATGTTAAGATATAATGGCTTTGAGGGGGGAAAAAATAAACCTAGGGGAGAGGGGAGTTTCCTGTAGTGCTG
1521 TTTCATTAGAGGATTTCAGTAAATTAAATTCCACAGCTAATTCAATAAATAATGGTACATTTAAGTGTTCTGATTTTAAT
1601 AATATATTTCACATTTATCCACACAGTAACAATGTAATATGTTAATGTAAATAAAATTGGTTTTGATACTCAGAAATAAC
1681 AAGAATTTAATTTTTTAAATTTGTTTACAGTCCTGGGAAAAGTAAGAATTATTTGCCAAAATAAGAGGAAAGAAAACCTT
1761 AGTATTATTAATGAGTTTACCATAGAATTGTTGGAAATACTGAAGACAGGTGCAATTTACTAAACTTTTGTTTTTAAACT
1841 ATTGTAGAGGCTGCATTAGAAGAAAATGTTTATAATGACAGAGCAACTATGACTATATAAAAAAGCTGAAATTAGAACTG
1921 TGTTTAGAAATAGATCAGTAACCCAGTGCCAAGGATGCCAAGCTGCCACCATGGTCTTGGCTCTCCCACAACCCAGTGTT
2001 TCTGGGGTAAGTTTCACAGTTTCTAGGCCCTGGAATAGCAGGCAGTGTAAGCCTTTGATAACTTTAGTTCGATGTTTTTC
2081 TTGTTTTTGTTTGTTGGTTTGGTGCATATGATAGTGGGTGTTATGCTATTTTGCTCTTCCCATCAAAATAAAGAAACTTC
2161 CAGAGGTTTACTGTTAAAAATACTGATATTTCCATAAACGGGTTTACCAAGGGTGTAGTATTTCATACCGCCTGAAATGA
2241 TCAGCATTGGCACAAATCAAAATTCAGCCGCCTTTGAAATGCAAAAATACCTTTGACTAGTAAGTACATCCTAGGAGTTT
2321 GAAAACTTAACTAAGGTTTAAAATTTACCTTGTTTAAAGAACTTCTGACTTTTGAGGAAAATCTAGCTTTCCAAGTAACT
2401 AAAATGTACATGAGATAAACCTCTCACCACTATGTGTCCCTTGAGAAATGCAACACTTTTTTAGTCTTCATACTTGTAAT
2481 CTATAAAAGAAATTCTGAAGTTTAGACCAAGTTGCCCATTTCTGCGTAATTGACATAAGTTCTGTTAAAAATATTATAAG
2561 TAATTCGTTTCGGTTTGTAGATGTTTCCCCTGACTTGTTAAAGAGGAAACCAGGAACTCAGTCATGTTTTTGTCCTGGAT
2641 AATCTACCTGTTATGCCAGTACTCCCATCCGAGGGGCATGCCCTTAGTTGCCCAGATGGAGATGCAGTTCAGTAGATTTG
2721 GGGCAAAGTGGCTACAGCTCTGTCTTCCATTCACTCAACACCTGTTCATGACTGAGCCAGGTGCCCAGGACACATCCTAA
2801 ACAGTCAGCTTCTATCCTGTGTCCTAGTTGGGGAGACAGAGTGCCAGCCAGCAACCCTCCCAGGTTTGTAGGTTTTAGGG
2881 GTTTTCAGTTTTGTTTGGGTTTTTTGTTTTTTGTTTTTGTTTCTACATCCTTCCCCGACTCCCAGGCATAATGAGGCATG
2961 TCTTACTCAATGTTATGCAATGGATTTAGGCAAAAATTCATTCTTAGTGTCAGCCACACAATTTTTTTTAATGCAGTATA
3041 TTCACCTGTAAATAGTTTGTGTAAAATTTGACAAAAAAAGTATATTTACTATACTGTAAATATATGTGATGATATATTGT
3121 ATTATTTTGCTTTTTTGTAAAGCAGTTAGTTGCTGCACATGGATAACAACAAAAATTTGATTATTCTCGTGTTAGTATTG
3201 TTAACTTCTTTTTGCGACTGCGTTACATCATTTAAAGAAAATGCTGTGTATTGTAAACTTAAATTGTATATGATAACTTA
3281 CTGTCCTTTCCATCCGGGCCTAAACTTTGGCAGTTCCTTTGTCTACAACCTTGTTAATACTGTAAACAGTTGTACGCCAG
3361 CAGGAAAAATACTGCCCAACAGACAAAATCGATCATTGTAGGGGAAAATCATAGAAATCCATTTCAGATCTTTATTGTTC
3441 CTCACCCCATTTTCCTCCTTGTGTATGTACTTCCCCCACCCCCCTTTTTTTAAGTAAAATGTAAATTCAATCTGCTCTAA
3521 GA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' guGUUUAAGCCAAGAUGUCCCAu 5'
            | |||||   |  :|||||| 
Target 5' aaCTAATTC---TGAGCAGGGTa 3'
901 - 920 138.00 -13.20
2
miRNA  3' guGUUUAAGCCAAGAUG--UCCCAu 5'
            || |  ||| |:|||  ||||| 
Target 5' tcCATAAACGGGTTTACCAAGGGTg 3'
2191 - 2215 129.00 -18.20
3
miRNA  3' guguuuAAGCCAAGAUGUCCCAu 5'
                |||  | |||||| || 
Target 5' cccttcTTC-ATCCTACAGAGTa 3'
383 - 404 124.00 -8.90
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-10b-5p :: NF1    [ Functional MTI ]
Validation Method GFP reporter assay , Luciferase reporter assay , Microarray , qRT-PCR , Other
Conditions ST88-14 , T265p21 , STS26T , SBC5 , SBC3
Disease neurofibromatosis type 1;
Location of target site 3'UTR
Tools used in this research TargetScan
Original Description (Extracted from the article) ... Co-transfection in 293T cells with either the miR-10b expression vector or a control vector showed that overexpression of miR-10b significantly reduced lucifer- ase activity from the construct containing NF1 3'UTR (Fig. 5b; P < 0.05), while miR-10b failed to reduce luciferase activity if its target sequence in the NF1 3'UTR was mutated (Fig. 5b). This result indicates that miR-10b directly targets 3'UTR of NF1 mRNA.// ...

- Chai, G. Liu, N. Ma, J. Li, H. Oblinger, J. et al., 2010, Cancer Sci.

Article - Chai, G. Liu, N. Ma, J. Li, H. Oblinger, J. et al.
- Cancer Sci, 2010
MicroRNAs (miRNAs) are frequently deregulated in human tumors, and play important roles in tumor development and progression. The pathological roles of miRNAs in neurofibromatosis type 1 (NF1) tumorigenesis are largely unknown. We demonstrated that miR-10b was up-regulated in primary Schwann cells isolated from NF1 neurofibromas and in cell lines and tumor tissues from malignant peripheral nerve sheath tumors (MPNSTs). Intriguingly, a significantly high level of miR-10b correlated with low neurofibromin expression was found in a neuroectodermal cell line: Ewing's sarcoma SK-ES-1 cells. Antisense inhibiting miR-10b in NF1 MPNST cells reduced cell proliferation, migration and invasion. Furthermore, we showed that NF1 mRNA was the target for miR-10b. Overexpression of miR-10b in 293T cells suppressed neurofibromin expression and activated RAS signaling. Antisense inhibition of miR-10b restored neurofibromin expression in SK-ES-1 cells, and decreased RAS signaling independent of neurofibromin in NF1 MPNST cells. These results suggest that miR-10b may play an important role in NF1 tumorigenesis through targeting neurofibromin and RAS signaling. (Cancer Sci 2010).
LinkOut: [PMID: 20550523]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
124 hsa-miR-10b-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT002021 HOXD10 homeobox D10 3 5
MIRT003216 KLF4 Kruppel-like factor 4 (gut) 3 1
MIRT005068 PPARA peroxisome proliferator-activated receptor alpha 4 1
MIRT005455 NCOR2 nuclear receptor corepressor 2 3 1
MIRT005504 NF1 neurofibromin 1 4 1
MIRT006294 PIEZO1 piezo-type mechanosensitive ion channel component 1 1 1
MIRT006366 BCL2L11 BCL2-like 11 (apoptosis facilitator) 2 1
MIRT006367 TFAP2C transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma) 2 1
MIRT006368 CDKN2A cyclin-dependent kinase inhibitor 2A 3 2
MIRT006369 CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1) 2 1
MIRT006538 TRA2B transformer 2 beta homolog (Drosophila) 1 1
MIRT006539 SRSF1 serine/arginine-rich splicing factor 1 1 1
MIRT006923 TP53 tumor protein p53 1 1
MIRT006924 NOTCH1 notch 1 1 1
MIRT006925 PAX6 paired box 6 1 1
MIRT007026 MAPRE1 microtubule-associated protein, RP/EB family, member 1 1 1
MIRT007072 SDC1 syndecan 1 1 1
MIRT025586 NR4A3 nuclear receptor subfamily 4, group A, member 3 1 1
MIRT025587 NRP2 neuropilin 2 1 1
MIRT047400 POLR2A polymerase (RNA) II (DNA directed) polypeptide A, 220kDa 1 1
MIRT047401 LEMD3 LEM domain containing 3 1 1
MIRT047402 EXTL3 exostoses (multiple)-like 3 1 1
MIRT047403 TXNDC16 thioredoxin domain containing 16 1 1
MIRT047404 STK33 serine/threonine kinase 33 1 1
MIRT047405 ZC3H18 zinc finger CCCH-type containing 18 1 1
MIRT047406 GBAS glioblastoma amplified sequence 1 1
MIRT047407 COPS5 COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis) 1 1
MIRT047408 ADRA1B adrenoceptor alpha 1B 1 1
MIRT047409 RTKN2 rhotekin 2 1 1
MIRT047410 COL6A2 collagen, type VI, alpha 2 1 1
MIRT047411 ZNF318 zinc finger protein 318 1 1
MIRT047412 INHBA inhibin, beta A 1 1
MIRT047413 SYT11 synaptotagmin XI 1 1
MIRT047414 SLC2A3 solute carrier family 2 (facilitated glucose transporter), member 3 1 1
MIRT047415 IGSF1 immunoglobulin superfamily, member 1 1 1
MIRT047416 IPCEF1 interaction protein for cytohesin exchange factors 1 1 1
MIRT047417 PPP1R13B protein phosphatase 1, regulatory subunit 13B 1 1
MIRT047418 APLNR apelin receptor 1 1
MIRT047419 MSL3 male-specific lethal 3 homolog (Drosophila) 1 1
MIRT047420 SLC25A30 solute carrier family 25, member 30 1 1
MIRT047421 CAMSAP3 calmodulin regulated spectrin-associated protein family, member 3 1 1
MIRT047422 XPO7 exportin 7 1 1
MIRT047423 STT3A STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) 1 1
MIRT047424 UROD uroporphyrinogen decarboxylase 1 1
MIRT047425 SMCHD1 structural maintenance of chromosomes flexible hinge domain containing 1 1 1
MIRT047426 GLB1L3 galactosidase, beta 1-like 3 1 1
MIRT047427 FEM1B fem-1 homolog b (C. elegans) 1 1
MIRT047428 CLPTM1 cleft lip and palate associated transmembrane protein 1 1 1
MIRT047429 FAM13A family with sequence similarity 13, member A 1 1
MIRT047430 HSPA1B heat shock 70kDa protein 1B 1 1
MIRT047431 CSNK2A1 casein kinase 2, alpha 1 polypeptide 1 1
MIRT047432 KIF21A kinesin family member 21A 1 1
MIRT047433 HK1 hexokinase 1 1 1
MIRT047434 EIF1 eukaryotic translation initiation factor 1 1 1
MIRT047435 AP3M1 adaptor-related protein complex 3, mu 1 subunit 1 1
MIRT047436 HIST1H2AL histone cluster 1, H2al 1 1
MIRT047437 ARFGEF1 ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) 1 1
MIRT047438 RUNDC3B RUN domain containing 3B 1 1
MIRT047439 PRPF8 PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) 1 1
MIRT047440 ND6 NADH dehydrogenase, subunit 6 (complex I) 1 1
MIRT047441 SCMH1 sex comb on midleg homolog 1 (Drosophila) 1 1
MIRT047442 LRRC59 leucine rich repeat containing 59 1 1
MIRT047443 ERLIN1 ER lipid raft associated 1 1 1
MIRT047444 FUT6 fucosyltransferase 6 (alpha (1,3) fucosyltransferase) 1 1
MIRT047445 CDK2 cyclin-dependent kinase 2 1 1
MIRT047446 DDHD2 DDHD domain containing 2 1 1
MIRT047447 PTPRT protein tyrosine phosphatase, receptor type, T 1 1
MIRT047448 UBE2Z ubiquitin-conjugating enzyme E2Z 1 1
MIRT047449 ORAI2 ORAI calcium release-activated calcium modulator 2 1 1
MIRT047450 ACLY ATP citrate lyase 1 1
MIRT047451 PARL presenilin associated, rhomboid-like 1 1
MIRT047452 DIAPH2 diaphanous homolog 2 (Drosophila) 1 1
MIRT047453 RPS9 ribosomal protein S9 1 1
MIRT047454 FASN fatty acid synthase 1 1
MIRT047455 ESYT1 extended synaptotagmin-like protein 1 1 1
MIRT047456 TENM3 teneurin transmembrane protein 3 1 1
MIRT047457 GRINA glutamate receptor, ionotropic, N-methyl D-aspartate-associated protein 1 (glutamate binding) 1 1
MIRT047458 TUBA1B tubulin, alpha 1b 1 1
MIRT047459 CDK12 cyclin-dependent kinase 12 1 1
MIRT047460 PPP3CB protein phosphatase 3, catalytic subunit, beta isozyme 1 1
MIRT047461 RPS8 ribosomal protein S8 1 1
MIRT047462 ATG13 autophagy related 13 1 1
MIRT047463 PIK3CD phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit delta 1 1
MIRT047464 C9orf114 chromosome 9 open reading frame 114 1 1
MIRT047465 PLA2G4F phospholipase A2, group IVF 1 1
MIRT047466 USP3 ubiquitin specific peptidase 3 1 1
MIRT047467 SLC25A5 solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5 1 1
MIRT047468 DMWD dystrophia myotonica, WD repeat containing 1 1
MIRT047469 HNRNPF heterogeneous nuclear ribonucleoprotein F 1 1
MIRT047470 RGP1 RGP1 retrograde golgi transport homolog (S. cerevisiae) 1 1
MIRT047471 PFAS phosphoribosylformylglycinamidine synthase 1 1
MIRT047472 KBTBD6 kelch repeat and BTB (POZ) domain containing 6 1 1
MIRT047473 LAMC1 laminin, gamma 1 (formerly LAMB2) 1 1
MIRT047474 SURF6 surfeit 6 1 1
MIRT047475 GLOD4 glyoxalase domain containing 4 1 1
MIRT047476 PRMT7 protein arginine methyltransferase 7 1 1
MIRT047477 MSTO1 misato homolog 1 (Drosophila) 1 1
MIRT047478 NEK7 NIMA (never in mitosis gene a)-related kinase 7 1 1
MIRT047479 EPHA4 EPH receptor A4 1 1
MIRT047480 ACTG1 actin, gamma 1 1 1
MIRT047481 KIAA0100 KIAA0100 1 1
MIRT047482 TSPAN33 tetraspanin 33 1 1
MIRT047483 C6orf106 chromosome 6 open reading frame 106 1 1
MIRT047484 THUMPD1 THUMP domain containing 1 1 1
MIRT047485 DLAT dihydrolipoamide S-acetyltransferase 1 1
MIRT047486 LRP3 low density lipoprotein receptor-related protein 3 1 1
MIRT047487 AASDH aminoadipate-semialdehyde dehydrogenase 1 1
MIRT047488 SETD7 SET domain containing (lysine methyltransferase) 7 1 1
MIRT047489 RBM17 RNA binding motif protein 17 1 1
MIRT047490 GK glycerol kinase 1 1
MIRT047491 LMF2 lipase maturation factor 2 1 1
MIRT047492 CHPF2 chondroitin polymerizing factor 2 1 1
MIRT047493 CTBP1 C-terminal binding protein 1 1 1
MIRT047494 UHRF1 ubiquitin-like with PHD and ring finger domains 1 1 1
MIRT047495 WHSC1L1 Wolf-Hirschhorn syndrome candidate 1-like 1 1 1
MIRT047496 RALBP1 ralA binding protein 1 1 1
MIRT047497 NAP1L1 nucleosome assembly protein 1-like 1 1 1
MIRT047498 LSS lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) 1 1
MIRT047499 NPTX1 neuronal pentraxin I 1 1
MIRT047500 PRRC2C proline-rich coiled-coil 2C 1 1
MIRT047501 PABPC1 poly(A) binding protein, cytoplasmic 1 1 1
MIRT047502 PSMD11 proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 1 1
MIRT047503 PSMD13 proteasome (prosome, macropain) 26S subunit, non-ATPase, 13 1 1
MIRT047504 NUFIP2 nuclear fragile X mental retardation protein interacting protein 2 1 1