Accession ID: MIRT005504 [miRNA, hsa-miR-10b :: NF1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-10b LinkOut: [miRBase ]
Synonyms MIRN10B, hsa-mir-10b, miRNA10B, mir-10b, MIR10B
Description Homo sapiens miR-10b stem-loop
Comment This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-10b-3p
Mature Sequence 66| ACAGAUUCGAUUCUAGGGGAAU |87
Evidence Experimental
Experiments Cloned
Putative hsa-miR-10b-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-10b-5p
Mature Sequence 27| UACCCUGUAGAACCGAAUUUGUG |49
Evidence Experimental
Experiments Cloned
Putative hsa-miR-10b-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol NF1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms DKFZp686J1293, FLJ21220, NFNS, VRNF, WSS
Description neurofibromin 1
Transcript NM_000267   LinkOut: [ RefSeq ]
Other Transcripts NM_001042492, NM_001128147   
Expression LinkOut: [ BioGPS ]
KEGG Pathway hsa04010    MAPK signaling pathway - Homo sapiens (human)
Putative miRNA Targets on NF1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of NF1
(miRNA target sites are highlighted)		 
>NF1|NM_000267|3'UTR
   1 TGAAGCTTGCTTGCTTTCTTTTTTAAAATCAACTTAACATGGGCTCTTCACTAGTGACCCCTTCCCTGTCCTTGCCCTTT
  81 CCCCCCATGTTGTAATGCTGCACTTCCTGTTTTATAATGAACCCATCCGGTTTGCCATGTTGCCAGATGATCAACTCTTC
 161 GAAGCCTTGCCTAAATTTAATGCTGCCTTTTCTTTAACTTTTTTTCTTCTACTTTTGGCGTGTATCTGGTATATGTAAGT
 241 GTTCAGAACAACTGCAAAGAAAGTGGGAGGTCAGGAAACTTTTAACTGAGAAATCTCAATTGTAAGAGAGGATGAATTCT
 321 TGAATACTGCTACTACTGGCCAGTGATGAAAGCCATTTGCACAGAGCTCTGCCTTCTGTGGTTTTCCCTTCTTCATCCTA
 401 CAGAGTAAAGTGTTAGTCCTATTTATACATTTTTCAAGATACAAGTTTATGAGAGAAATAGTATTATAACCCCAGTATGT
 481 TTAATCTTTTAGCTGTGGACTTTTTTTTTAACCGTACAAAACTGAAAGAACCATAGAGGTCAAGCCTCAGTGACTTGACA
 561 CCATAAAGCCACAGACAAGGTACTTGGGGGGGAGGGCAGGGAAATTTCATATTTTATAGTGGATTCTTAAGAAATACTAA
 641 CACTTGAGTATTAGCAATAATTACAGGAAAATAAGTGCGACCACATATATCTTAACATTACTGAATTAAAACTATGGCTT
 721 CTAAGTCCTTATCCAAACTCAGTCATCCAAACTAGTTTATTTTTTTCTCCAGTTGATTATCTTTTAATTTTTAATTTTGC
 801 TAAAGGTGGTTTTTTTGTGTTTTGTTTTTTGTAAACCAAAACTATACTAAGTATAGTAATTATATATATATATATATTTT
 881 TTCCCCTCCCCCTCTTCTTTCCTAACTAATTCTGAGCAGGGTAATCAGTGAACAAAGTGTTGAAAATTGTTCCCAGAAGG
 961 TAATTTTCATAGATGTTTGCATTAGCTCCATAGCAAAATGGAATGGTACGTGACATTTAGGGTAGCTGATATTTTTATTT
1041 TGTTAAATAATTTCCAAGAATAGAGTATGGTGTATATTATAAATTTCTTTGATAAGATGTATTTTGAATGTCTTTTAATC
1121 TTCCTCCTCCTCTCCAAAAAAATCAGAAACCTCTTTAAGAAAACATGTAGGTTATATATGCTAGAATTGCATTTAATCAC
1201 TGTGAAAAGACTGGTCAGCCTGCATTAGTATGACAGTAGGGGGGCTGTTAGAATTGCTGCTATACTGGTGGTATGGATTA
1281 TCATGGCATTGGAATTTTCATAGTAATGCAGATCCAATTTCTTTGTGGTACCTGCAGTTTACAAAATAATTTGACTTCAG
1361 TGAGCATATTGGTATCTGGATGTTCCAATTTAGAACTAAACCATATTTATTACAAAAAGATATTAATCCCTCTACTCCCA
1441 GGTTCCCTTTATATGTTAAGATATAATGGCTTTGAGGGGGGAAAAAATAAACCTAGGGGAGAGGGGAGTTTCCTGTAGTG
1521 CTGTTTCATTAGAGGATTTCAGTAAATTAAATTCCACAGCTAATTCAATAAATAATGGTACATTTAAGTGTTCTGATTTT
1601 AATAATATATTTCACATTTATCCACACAGTAACAATGTAATATGTTAATGTAAATAAAATTGGTTTTGATACTCAGAAAT
1681 AACAAGAATTTAATTTTTTAAATTTGTTTACAGTCCTGGGAAAAGTAAGAATTATTTGCCAAAATAAGAGGAAAGAAAAC
1761 CTTAGTATTATTAATGAGTTTACCATAGAATTGTTGGAAATACTGAAGACAGGTGCAATTTACTAAACTTTTGTTTTTAA
1841 ACTATTGTAGAGGCTGCATTAGAAGAAAATGTTTATAATGACAGAGCAACTATGACTATATAAAAAAGCTGAAATTAGAA
1921 CTGTGTTTAGAAATAGATCAGTAACCCAGTGCCAAGGATGCCAAGCTGCCACCATGGTCTTGGCTCTCCCACAACCCAGT
2001 GTTTCTGGGGTAAGTTTCACAGTTTCTAGGCCCTGGAATAGCAGGCAGTGTAAGCCTTTGATAACTTTAGTTCGATGTTT
2081 TTCTTGTTTTTGTTTGTTGGTTTGGTGCATATGATAGTGGGTGTTATGCTATTTTGCTCTTCCCATCAAAATAAAGAAAC
2161 TTCCAGAGGTTTACTGTTAAAAATACTGATATTTCCATAAACGGGTTTACCAAGGGTGTAGTATTTCATACCGCCTGAAA
2241 TGATCAGCATTGGCACAAATCAAAATTCAGCCGCCTTTGAAATGCAAAAATACCTTTGACTAGTAAGTACATCCTAGGAG
2321 TTTGAAAACTTAACTAAGGTTTAAAATTTACCTTGTTTAAAGAACTTCTGACTTTTGAGGAAAATCTAGCTTTCCAAGTA
2401 ACTAAAATGTACATGAGATAAACCTCTCACCACTATGTGTCCCTTGAGAAATGCAACACTTTTTTAGTCTTCATACTTGT
2481 AATCTATAAAAGAAATTCTGAAGTTTAGACCAAGTTGCCCATTTCTGCGTAATTGACATAAGTTCTGTTAAAAATATTAT
2561 AAGTAATTCGTTTCGGTTTGTAGATGTTTCCCCTGACTTGTTAAAGAGGAAACCAGGAACTCAGTCATGTTTTTGTCCTG
2641 GATAATCTACCTGTTATGCCAGTACTCCCATCCGAGGGGCATGCCCTTAGTTGCCCAGATGGAGATGCAGTTCAGTAGAT
2721 TTGGGGCAAAGTGGCTACAGCTCTGTCTTCCATTCACTCAACACCTGTTCATGACTGAGCCAGGTGCCCAGGACACATCC
2801 TAAACAGTCAGCTTCTATCCTGTGTCCTAGTTGGGGAGACAGAGTGCCAGCCAGCAACCCTCCCAGGTTTGTAGGTTTTA
2881 GGGGTTTTCAGTTTTGTTTGGGTTTTTTGTTTTTTGTTTTTGTTTCTACATCCTTCCCCGACTCCCAGGCATAATGAGGC
2961 ATGTCTTACTCAATGTTATGCAATGGATTTAGGCAAAAATTCATTCTTAGTGTCAGCCACACAATTTTTTTTAATGCAGT
3041 ATATTCACCTGTAAATAGTTTGTGTAAAATTTGACAAAAAAAGTATATTTACTATACTGTAAATATATGTGATGATATAT
3121 TGTATTATTTTGCTTTTTTGTAAAGCAGTTAGTTGCTGCACATGGATAACAACAAAAATTTGATTATTCTCGTGTTAGTA
3201 TTGTTAACTTCTTTTTGCGACTGCGTTACATCATTTAAAGAAAATGCTGTGTATTGTAAACTTAAATTGTATATGATAAC
3281 TTACTGTCCTTTCCATCCGGGCCTAAACTTTGGCAGTTCCTTTGTCTACAACCTTGTTAATACTGTAAACAGTTGTACGC
3361 CAGCAGGAAAAATACTGCCCAACAGACAAAATCGATCATTGTAGGGGAAAATCATAGAAATCCATTTCAGATCTTTATTG
3441 TTCCTCACCCCATTTTCCTCCTTGTGTATGTACTTCCCCCACCCCCCTTTTTTTAAGTAAAATGTAAATTCAATCTGCTC
3521 TAAGAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' uaAGGGGAUCUUAGCUUAGACa 5'
            |::|| ||||| ||:| || 
Target 5' aaTTTCCAAGAATAGAGTATGg 3'
 		1049 - 1070 128.00 -12.20
2 
miRNA  3' uaaggggAUCUUAGCUUAGACa 5'
                 || |||: |||||| 
Target 5' taaaatgTA-AATTCAATCTGc 3'
 		3498 - 3518 126.00 -5.50
3 
miRNA  3' uaaGGGGAUCUUAGCUUAGACa 5'
             |:::|:| :| | ||||| 
Target 5' ctaCTTTTGGCGT-GTATCTGg 3'
 		209 - 229 122.00 -12.10
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-10b :: NF1    [ Functional MTI ]
Validation Method GFP reporter assay , Luciferase reporter assay , Microarray , qRT-PCR
Conditions ST88-14, T265p21, STS26T, SBC5, SBC3
Location of target site 3'UTR
Tools used in this research TargetScan
Original Description (Extracted from the article) ... Co-transfection in 293T cells with either the miR-10b expression vector or a control vector showed that overexpression of miR-10b significantly reduced lucifer- ase activity from the construct containing NF1 3'UTR (Fig. 5b; P < 0.05), while miR-10b failed to reduce luciferase activity if its target sequence in the NF1 3'UTR was mutated (Fig. 5b). This result indicates that miR-10b directly targets 3'UTR of NF1 mRNA.// ...

- Chai, G. Liu, N. Ma, J. Li, H. Oblinger, J. et al., 2010, Cancer Sci.
miRNA-target interactions (Provided by authors)
IDDuplex structurePosition
1 
miRNA  3' guGUUUAAGCCAAGAUGUCCCAu 5'
            | |||||   |  :|||||| 
Target 5' -aCTAATTC---TGAGCAGGGTa 3'
 		1 - 19
Article - Chai, G. Liu, N. Ma, J. Li, H. Oblinger, J. et al.
- Cancer Sci, 2010
MicroRNAs (miRNAs) are frequently deregulated in human tumors, and play important roles in tumor development and progression. The pathological roles of miRNAs in neurofibromatosis type 1 (NF1) tumorigenesis are largely unknown. We demonstrated that miR-10b was up-regulated in primary Schwann cells isolated from NF1 neurofibromas and in cell lines and tumor tissues from malignant peripheral nerve sheath tumors (MPNSTs). Intriguingly, a significantly high level of miR-10b correlated with low neurofibromin expression was found in a neuroectodermal cell line: Ewing's sarcoma SK-ES-1 cells. Antisense inhibiting miR-10b in NF1 MPNST cells reduced cell proliferation, migration and invasion. Furthermore, we showed that NF1 mRNA was the target for miR-10b. Overexpression of miR-10b in 293T cells suppressed neurofibromin expression and activated RAS signaling. Antisense inhibition of miR-10b restored neurofibromin expression in SK-ES-1 cells, and decreased RAS signaling independent of neurofibromin in NF1 MPNST cells. These results suggest that miR-10b may play an important role in NF1 tumorigenesis through targeting neurofibromin and RAS signaling. (Cancer Sci 2010).
LinkOut: [PMID: 20550523]