Accession ID: MIRT005517 [miRNA, hsa-miR-409-3p :: FGB, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-409LinkOut: [miRBase ]
Synonyms MIRN409, hsa-mir-409, MIR409
Description Homo sapiens miR-409 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies . The 5' end of the miRNA may be offset with respect to previous annotations.
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-409-3p
Evidence Experimental
Experiments Cloned
Putative hsa-miR-409-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol FGB LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms MGC104327, MGC120405
Description fibrinogen beta chain
Transcript NM_0051    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on FGB LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of FGB
(miRNA target sites are highlighted)
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
               | :|: :|| ||||||| 
Target 5' tcttcTGTATGTGA-CAACATTt 3'
26 - 47 144.00 -8.10
miRNA  3' ucccCAAGUGG--C-UCGUUGUaag 5'
              | |||||  | ||:||||   
Target 5' tcttGCTCACCAAGAAGTAACAaaa 3'
191 - 215 108.00 -9.72
miRNA  3' uccccaaGUGGC-UCGUUGUaag 5'
                 | |:|  ||||:|   
Target 5' aaccagaCTCTGTTGCAATAaaa 3'
419 - 441 91.00 -6.62
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-409-3p :: FGB    [ Functional MTI ]
Validation Method ELISA , Flow , Luciferase reporter assay , Other
Conditions HuH7 , HEK-293T
Location of target site 3'UTR
Tools used in this research RNA22 , TargetScan
Original Description (Extracted from the article) ... overexpression of hsa-miR-409-3p specifically lowered fibrinogen Bβ (FGB) mRNA levels and this effect was dependent on a target site in the FGB mRNA 3'UTR.// ...

- Fort, A. Borel, C. Migliavacca, E. et al., 2010, Blood.

Article - Fort, A. Borel, C. Migliavacca, E. et al.
- Blood, 2010
Elevated levels of fibrinogen are associated with increased risk of cardiovascular disease, whereas low fibrinogen can lead to a bleeding disorder. We investigated whether microRNAs (miRNAs), known to act as post-transcriptional regulators of gene expression, regulate fibrinogen production. Using transfection of a library of 470 annotated human miRNA precursor molecules in HuH7 hepatoma cells, and quantitative measurements of fibrinogen production, we identified 23 miRNAs with down-regulating (up to 64% decrease) and 4 with up-regulating effects (up to 129% increase) on fibrinogen production. Among the downregulating miRNAs, we investigated the mechanism of action of three hsa-miR-29 family members and hsa-miR-409-3p. Overexpression of hsa-miR-29 members lead to decreased steady-state levels of all fibrinogen gene (FGA, FGB, FGG) transcripts in HuH7 cells. Luciferase reporter gene assays demonstrated that this was independent of miRNA-fibrinogen 3'UTR interactions. In contrast, overexpression of hsa-miR-409-3p specifically lowered fibrinogen Bbeta (FGB) mRNA levels and this effect was dependent on a target site in the FGB mRNA 3'UTR. This study adds to the known mechanisms that control fibrinogen production, points towards a potential cause of variable circulating fibrinogen levels, and demonstrates that a screening approach can identify miRNAs that regulate clinically important proteins.
LinkOut: [PMID: 20570858]
MiRNA-Target Expression Profile:

MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
7 hsa-miR-409-3p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT005517 FGB fibrinogen beta chain 2 1
MIRT005518 FGA fibrinogen alpha chain 2 1
MIRT005519 FGG fibrinogen gamma chain 2 1
MIRT006452 PHF10 PHD finger protein 10 3 1
MIRT006649 ANG angiogenin, ribonuclease, RNase A family, 5 3 1
MIRT006807 RDX radixin 3 1
MIRT007285 IFNG interferon, gamma 1 1