Accession ID: MIRT005551 [miRNA, hsa-miR-214 :: POU4F2, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-214 LinkOut: [miRBase ]
Synonyms MIRN214, miRNA214, mir-214, MIR214
Description Homo sapiens miR-214 stem-loop
Comment This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-214-3p
Mature Sequence 71| ACAGCAGGCACAGACAGGCAGU |92
Evidence Experimental
Experiments Cloned
Putative hsa-miR-214-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-214-5p
Mature Sequence 30| UGCCUGUCUACACUUGCUGUGC |51
Evidence Experimental
Experiments Cloned
Putative hsa-miR-214-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol POU4F2 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms BRN3.2, BRN3B, Brn-3b
Description POU class 4 homeobox 2
Transcript NM_004575   LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on POU4F2 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of POU4F2
(miRNA target sites are highlighted)		 
>POU4F2|NM_004575|3'UTR
   1 TAGAAGACTCTTGGCCTCTCCAGAGACGCCCCTTTCCTCGTCCGCTCTTTTCTCTCCTCTCTTCTGCCTCTTTTCACTTT
  81 TGGCGACTAGAAACAATTCCAGTAAATGTGAATCTCGACAAATCGAGGACTGAAGAGGGAGCGAACGAGCGAACAACTGA
 161 GCCCAAGCCGGTGAGAATGTGAAACAGTTTCTCAAAGGAAAGAATAACAAAAGATGGTATTTGTCTGTTGTAGCAAAGTT
 241 GTCCCTTTGAACCCCACCTCGGCTTCTTCAGAGGAAGTGTGGAGATGGCTGTTTGCAGGAAGGCAGACGAGACAGTGTTT
 321 AAAAAGTCCACAAGAATGATCAAGTAAGATTTGTTTTTATTCTTACAGACATCACCCGTGTTCAAGTTTAAAAGTACACT
 401 TTGCAACTATTTTTCAGAAATAGAAATTGATTCAGGACTAAAACTTTAAACTAGAGTTGATGCTTAATGTGATAGAGACA
 481 TCTCTAAAGTATTTTGAATTTTAAAAAAAGATGGCAGATTTTCTGCATTTACACTGTATATTATATATATATTTTTATTG
 561 TGGTTCTTACCCCCTTTTCCTTCTCTGAAGTGTTAATGCTTAAGAAAAGAGTTGCGCCTGCTGTGTTCACTGATCTTGAA
 641 AGCTATTATTAGATTATTGCAGAACAACCCTCTGTAAATTATTAATTTATCTCTCTAGCAACTTAATTTTGTGCACATTC
 721 TAATTAATTAAACTTCTTCCGTCTAAAAAAAGTGGGGGAAATGTATAGCTAGTAACGTTCAAAAAATTTTGTTTGATGAG
 801 TTTACCGAATTTTTACAGCTTTCCTCCTATACTGTGTTCCTTTTGACCCATTTGTATATTCTCACTTGAATGAAGATTGT
 881 TTTTTTCTTTGTTTTTACTGGTAGTGTTCTGATTTGTGAGTCGACACTCAGTAATGGATGTCTTAATCGTGTAGACCTGA
 961 TTCACTGTCTGAAGTATTGTTTACTTCGTTACATATTTAATGGGGATTCCCACATTGTCCCCATGACACATGAGCGCTCT
1041 CACTTACCCTTACACACACACACACACACACACACACCTCTAACAGAAGGGAAGAAGCAGTTGGAAGCATGACCGATGCA
1121 CCATTTTCTAGTTTTAGGTGCATTTGCCACTTGGTGTTTGCCCTTCAGATTTTAGATTTCACCAAGGTATTTCAGTCTTC
1201 CAGTTTTCAATTGCTTTGTTGGCTACATGTTAATATTTATAGGAATACTTCAGTTTTTCCTTTTGGAGGTTTGTTTGTAG
1281 AAAAACTAATTTGAACTATAAGAAAGACAGTGCACTGCTTGTAAATTCACATTGTTTGGAAAAATTCTTTTGGAACAAAA
1361 AATTAGGTACATGATAACTGGTACCTTATCTACTGTAAATATTTCATTAAAAATGATGCACACATAGATATATTCTTACA
1441 AATTTTGCTGTATTGCTGTTCTCTTTGAGGCTCTCCAAAGTCTTGAGTTCTGTATATGGCCTGGTTTCTTGTTTTTATTA
1521 ATAGATGGTTTATTTACTATGGTAATGTATTAATTTATTTTTGGTGTTGTTCGATTGTCTTTCATTGAAGAGATAATTTT
1601 AATGTTTTATTGGCAACGTATGCTGCTTTTTCATTAAAATATGCTATTAAAATTAAATGGCTTTTA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' ugacggaCAGACACGGACGACa 5'
                 || || |||||||| 
Target 5' gaaaagaGT-TGCGCCTGCTGt 3'
 		604 - 624 154.00 -18.10
2 
miRNA  3' ugacggaCAGACACGGACGACa 5'
                 || | ||:|||:|| 
Target 5' aaagatgGTATTTGTCTGTTGt 3'
 		210 - 231 127.00 -13.20
3 
miRNA  3' ugacGGACA-GACAC--GGACGACa 5'
              ||| | |||||  ||| :|| 
Target 5' tcctCCTATACTGTGTTCCTTTTGa 3'
 		822 - 846 116.00 -12.90
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-214 :: POU4F2    [ Functional MTI ]
Validation Method Flow , GFP reporter assay , Northern blot , qRT-PCR , Western blot
Conditions RGC-5, MCF-7, ND-7
Location of target site 3'UTR
Original Description (Extracted from the article) ... As shown in Fig. 3A, GFP levels are reduced in RGC-5 cells only upon the co-transfection of mir-23 and mir-214. In contrast, the GFP::3b-3'UTR construct is down-regulated by mir-23, mir-214 or a combination of both in the ND7 neuroblastoma cell line.// ...

- Calissano, M. Latchman, D. S., 2010, Mol Cell Neurosci.
Article - Calissano, M. Latchman, D. S.
- Mol Cell Neurosci, 2010
We have previously shown that the Brn-3b transcription factor is subjected to post-transcriptional gene regulation by specific microRNAs (mir-23 and mir-214) in the ND7 and SHSY-5Y neuronal cell lines (Calissano et al., 2007). As Brn-3b plays an essential role in the survival of retinal ganglion cells in the rat (Erkman et al., 1996; Gan et al., 1996; Gan et al., 1999; Erkman et al., 2000), we wanted to investigate whether mir-23 and mir-214 are expressed and target Brn-3b mRNA in a retinal ganglion cell line (RGC-5) thus potentially killing the cells expressing it. Here we show that, possibly due to its pro-survival role, Brn-3b is protected from degradation by microRNAs in RGC-5 cells in contrast to its fate in other cell types. This seems to be accomplished by i) the lack of expression of one of the two microRNAs targeting its 3'UTR and by ii) the requirement of at least two distinct microRNAs to mediate its down-regulation in retinal ganglion cells. We speculate that this mechanism could have a widespread role in the regulation of mRNAs encoding for essential proteins.
LinkOut: [PMID: 20609388]