Accession ID: MIRT005559 [miRNA, hsa-miR-101-5p :: ATM, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-101-1LinkOut: [miRBase ]
Description Homo sapiens miR-101-1 stem-loop
Comment Reference .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-101-5p
Mature Sequence 11| CAGUUAUCACAGUGCUGAUGCU |32
Evidence Experimental
Experiments Cloned
Putative hsa-miR-101-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol ATM LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms AT1, ATA, ATC, ATD, ATDC, ATE, DKFZp781A0353, MGC74674, TEL1, TELO1
Description ataxia telangiectasia mutated
Transcript NM_0000    LinkOut: [ RefSeq ]
Other Transcripts NM_1382   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on ATM LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of ATM
(miRNA target sites are highlighted)
>ATM|NM_0000|3'UTR
   1 TGATCTTCAGTATATGAATTACCCTTTCATTCAGCCTTTAGAAATTATATTTTAGCCTTTATTTTTAACCTGCCAACATA
  81 CTTTAAGTAGGGATTAATATTTAAGTGAACTATTGTGGGTTTTTTTGAATGTTGGTTTTAATACTTGATTTAATCACCAC
 161 TCAAAAATGTTTTGATGGTCTTAAGGAACATCTCTGCTTTCACTCTTTAGAAATAATGGTCATTCGGGCTGGGCGCAGCG
 241 GCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGAGCGGATCACAAGGTCAGGAGTTCGAGACCAGCCTGGCCA
 321 AGAGACCAGCCTGGCCAGTATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCGAGCATGGTGGCGGGCACCT
 401 GTAATCCCAGCTACTCGAGAGGCTGAGGCAGGAGAATCTCTTGAACCTGGGAGGTGAAGGTTGCTGTGGGCCAAAATCAT
 481 GCCATTGCACTCCAGCCTGGGTGACAAGAGCGAAACTCCATCTCAAAAAAAAAAAAAAAAAAACAGAAACGTATTTGGAT
 561 TTTTCCTAGTAAGATCACTCAGTGTTACTAAATAATGAAGTTGTTATGGAGAACAAATTTCAAAGACACAGTTAGTGTAG
 641 TTACTATTTTTTTAAGTGTGTATTAAAACTTCTCATTCTATTCTCTTTATCTTTTAAGCCCTTCTGTACTGTCCATGTAT
 721 GTTATCTTTCTGTGATAACTTCATAGATTGCCTTCTAGTTCATGAATTCTCTTGTCAGATGTATATAATCTCTTTTACCC
 801 TATCCATTGGGCTTCTTCTTTCAGAAATTGTTTTTCATTTCTAATTATGCATCATTTTTCAGATCTCTGTTTCTTGATGT
 881 CATTTTTAATGTTTTTTTAATGTTTTTTATGTCACTAATTATTTTAAATGTCTGTACTTGATAGACACTGTAATAGTTCT
 961 ATTAAATTTAGTTCCTGCTGTTTATATCTGTTGATTTTTGTATTTGATAGGCTGTTCATCCAGTTTTGTCTTTTTGAAAA
1041 GTGAGTTTATTTTCAGCAAGGCTTTATCTATGGGAATCTTGAGTGTCTGTTTATGTCATATTCCCAGGGCTGTTGCTGCA
1121 CACAAGCCCATTCTTATTTTAATTTCTTGGCTTTAGGGTTTCCATACCTGAAGTGTAGCATAAATACTGATAGGAGATTT
1201 CCCAGGCCAAGGCAAACACACTTCCTCCTCATCTCCTTGTGCTAGTGGGCAGAATATTTGATTGATGCCTTTTTCACTGA
1281 GAGTATAAGCTTCCATGTGTCCCACCTTTATGGCAGGGGTGGAAGGAGGTACATTTAATTCCCACTGCCTGCCTTTGGCA
1361 AGCCCTGGGTTCTTTGCTCCCCATATAGATGTCTAAGCTAAAAGCCGTGGGTTAATGAGACTGGCAAATTGTTCCAGGAC
1441 AGCTACAGCATCAGCTCACATATTCACCTCTCTGGTTTTTCATTCCCCTCATTTTTTTCTGAGACAGAGTCTTGCTCTGT
1521 CACCCAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTGAAACCTCTGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCA
1601 GCCTCCCGAGTAGCTGGGACTACAGGCGTGTGCCAACACGCCCGGCTAATTTTTTGTATTTTTATTAGAGACGGAGTTTC
1681 ACCGTGTTAGCCAGGATGGTCTCGATCGCTTGACCTCGTGATCCACCCTCCTCGGCCTCCCAAAGTGCTGGGATTACAGG
1761 TGTGAGCCACCGCGCCCGGCCTCATTCCCCTCATTTTTGACCGTAAGGATTTCCCCTTTCTTGTAAGTTCTGCTATGTAT
1841 TTAAAAGAATGTTTTCTACATTTTATCCAGCATTTCTCTGTGTTCTGTTGGAAGGGAAGGGCTTAGGTATCTAGTTTGAT
1921 ACATAGGTAGAAGTGGAACATTTCTCTGTCCCCCAGCTGTCATCATATAAGATAAACATCAGATAAAAAGCCACCTGAAA
2001 GTAAAACTACTGACTCGTGTATTAGTGAGTATAATCTCTTCTCCATCCTTAGGAAAATGTTCATCCCAGCTGCGGAGATT
2081 AACAAATGGGTGATTGAGCTTTCTCCTCGTATTTGGACCTTGAAGGTTATATAAATTTTTTTCTTATGAAGAGTTGGCAT
2161 TTCTTTTTATTGCCAATGGCAGGCACTCATTCATATTTGATCTCCTCACCTTCCCCTCCCCTAAAACCAATCTCCAGAAC
2241 TTTTTGGACTATAAATTTCTTGGTTTGACTTCTGGAGAACTGTTCAGAATATTACTTTGCATTTCAAATTACAAACTTAC
2321 CTTGGTGTATCTTTTTCTTACAAGCTGCCTAAATGAATATTTGGTATATATTGGTAGTTTTATTACTATAGTAAATCAAG
2401 GAAATGCAGTAAACTTAAAATGTCTTTAAGAAAGCCCTGAAATCTTCATGGGTGAAATTAGAAATTATCAACTAGATAAT
2481 AGTATAGATAAATGAATTTGTAGCTAATTCTTGCTAGTTGTTGCATCCAGAGAGCTTTGAATAACATCATTAATCTACTC
2561 TTTAGCCTTGCATGGTATGCTATGAGGCTCCTGTTCTGTTCAAGTATTCTAATCAATGGCTTTGAAAAGTTTATCAAATT
2641 TACATACAGATCACAAGCCTAGGAGAAATAACTAATTCACAGATGACAGAATTAAGATTATAAAAGATTTTTTTTTTGTA
2721 ATTTTAGTAGAGACAGGGTTGCCATTGTATTCCAGCCTTGGCGACAGAGCAAGACTCTGCCTCAAAAAAAAAAAAAAAAA
2801 GGTTTTGGCAAGCTGGAACTCTTTCTGCAAATGACTAAGATAGAAAACTGCCAAGGACAAATGAGGAGTAGTTAGATTTT
2881 GAAAATATTAATCATAGAATAGTTGTTGTATGCTAAGTCACTGACCCATATTATGTACAGCATTTCTGATCTTTACTTTG
2961 CAAGATTAGTGATACTATCCCAATACACTGCTGGAGAAATCAGAATTTGGAGAAATAAGTTGTCCAAGGCAAGAAGATAG
3041 TAAATTATAAGTACAAGTGTAATATGGACAGTATCTAACTTGAAAAGATTTCAGGCGAAAAGAATCTGGGGTTTGCCAGT
3121 CAGTTGCTCAAAAGGTCAATGAAAACCAAATAGTGAAGCTATCAGAGAAGCTAATAAATTATAGACTGCTTGAACAGTTG
3201 TGTCCAGATTAAGGGAGATAATAGCTTTCCCACCCTACTTTGTGCAGGTCATACCTCCCCAAAGTGTTTACCTAATCAGT
3281 AGGTTCACAAACTCTTGGTCATTATAGTATATGCCTAAAATGTATGCACTTAGGAATGCTAAAAATTTAAATATGGTCTA
3361 AAGCAAATAAAAGCAAAGAGGAAAAACTTTGGACAGCGTAAAGACTAGAATAGTCTTTTAAAAAGAAAGCCAGTATATTG
3441 GTTTGAAATATAGAGATGTGTCCCAATTTCAAGTATTTTAATTGCACCTTAATGAAATTATCTATTTTCTATAGATTTTA
3521 GTACTATTGAATGTATTACTTTACTGTTACCTGAATTTATTATAAAGTGTTTTTGAATAAATAATTCTAAAAGC
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' ucGUAGUCGUGACACUAUUGAc 5'
            :|||  : ||||||||||| 
Target 5' gtTATC-TTTCTGTGATAACTt 3'
721 - 741 163.00 -16.00
2
miRNA  3' ucGUAGUCGUGACACUAUUGac 5'
            :||||  |||  |||||:  
Target 5' atTATCA--ACT-AGATAATag 3'
2464 - 2482 119.00 -5.70
3
miRNA  3' ucGUAGUCGUGACACUAU---UGAc 5'
            ::|| |:||| |||||   ||| 
Target 5' aaTGTCTGTACT-TGATAGACACTg 3'
927 - 950 119.00 -13.40
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-101-5p :: ATM    [ Functional MTI ]
Validation Method Luciferase reporter assay , qRT-PCR , Other
Conditions 293T , 95C , 95D , U87MGD , U87MG , M059K
Location of target site 3'UTR
Tools used in this research MicroCosm , miRanda , miRBase Target Database
Original Description (Extracted from the article) ... We identified that miR- 101 could efficiently target DNA-PKcs and ATM via binding to the 3'- UTR of DNA-PKcs or ATM mRNA.//To verify whether DNA-PKcs or ATM is a target of miR-101, we made a lentiviral construct that contained a precursor of miR-101 (Figure S1)//These results indicate for the first time that, besides mTOR, DNA-PK and ATM are also targets of miR-101.// ...

- Yan, D. Ng, W. L. Zhang, X. Wang, P. Zhang, et al., 2010, PLoS One.

Article - Yan, D. Ng, W. L. Zhang, X. Wang, P. Zhang, et al.
- PLoS One, 2010
BACKGROUND: Radiotherapy kills tumor-cells by inducing DNA double strand breaks (DSBs). However, the efficient repair of tumors frequently prevents successful treatment. Therefore, identifying new practical sensitizers is an essential step towards successful radiotherapy. In this study, we tested the new hypothesis: identifying the miRNAs to target DNA DSB repair genes could be a new way for sensitizing tumors to ionizing radiation. PRINCIPAL FINDINGS: HERE, WE CHOSE TWO GENES: DNA-PKcs (an essential factor for non-homologous end-joining repair) and ATM (an important checkpoint regulator for promoting homologous recombination repair) as the targets to search their regulating miRNAs. By combining the database search and the bench work, we picked out miR-101. We identified that miR-101 could efficiently target DNA-PKcs and ATM via binding to the 3'- UTR of DNA-PKcs or ATM mRNA. Up-regulating miR-101 efficiently reduced the protein levels of DNA-PKcs and ATM in these tumor cells and most importantly, sensitized the tumor cells to radiation in vitro and in vivo. CONCLUSIONS: These data demonstrate for the first time that miRNAs could be used to target DNA repair genes and thus sensitize tumors to radiation. These results provide a new way for improving tumor radiotherapy.
LinkOut: [PMID: 20617180]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
3 hsa-miR-101-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT005558 PRKDC protein kinase, DNA-activated, catalytic polypeptide 2 1
MIRT005559 ATM ataxia telangiectasia mutated 3 1
MIRT038714 TMSB10 thymosin beta 10 1 1