Accession ID: MIRT005573 [miRNA, hsa-miR-374a-5p :: ATM, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-374aLinkOut: [miRBase ]
Description Homo sapiens miR-374a stem-loop
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-374a-5p
Mature Sequence 12| UUAUAAUACAACCUGAUAAGUG |33
Evidence Experimental
Experiments Cloned
Putative hsa-miR-374a-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol ATM LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1
Description ataxia telangiectasia mutated
Transcript NM_0000    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on ATM LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of ATM
(miRNA target sites are highlighted)
>ATM|NM_0000|3'UTR
   1 TCTTCAGTATATGAATTACCCTTTCATTCAGCCTTTAGAAATTATATTTTAGCCTTTATTTTTAACCTGCCAACATACTT
  81 TAAGTAGGGATTAATATTTAAGTGAACTATTGTGGGTTTTTTTGAATGTTGGTTTTAATACTTGATTTAATCACCACTCA
 161 AAAATGTTTTGATGGTCTTAAGGAACATCTCTGCTTTCACTCTTTAGAAATAATGGTCATTCGGGCTGGGCGCAGCGGCT
 241 CACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGAGCGGATCACAAGGTCAGGAGTTCGAGACCAGCCTGGCCAAGA
 321 GACCAGCCTGGCCAGTATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCGAGCATGGTGGCGGGCACCTGTA
 401 ATCCCAGCTACTCGAGAGGCTGAGGCAGGAGAATCTCTTGAACCTGGGAGGTGAAGGTTGCTGTGGGCCAAAATCATGCC
 481 ATTGCACTCCAGCCTGGGTGACAAGAGCGAAACTCCATCTCAAAAAAAAAAAAAAAAAAACAGAAACGTATTTGGATTTT
 561 TCCTAGTAAGATCACTCAGTGTTACTAAATAATGAAGTTGTTATGGAGAACAAATTTCAAAGACACAGTTAGTGTAGTTA
 641 CTATTTTTTTAAGTGTGTATTAAAACTTCTCATTCTATTCTCTTTATCTTTTAAGCCCTTCTGTACTGTCCATGTATGTT
 721 ATCTTTCTGTGATAACTTCATAGATTGCCTTCTAGTTCATGAATTCTCTTGTCAGATGTATATAATCTCTTTTACCCTAT
 801 CCATTGGGCTTCTTCTTTCAGAAATTGTTTTTCATTTCTAATTATGCATCATTTTTCAGATCTCTGTTTCTTGATGTCAT
 881 TTTTAATGTTTTTTTAATGTTTTTTATGTCACTAATTATTTTAAATGTCTGTACTTGATAGACACTGTAATAGTTCTATT
 961 AAATTTAGTTCCTGCTGTTTATATCTGTTGATTTTTGTATTTGATAGGCTGTTCATCCAGTTTTGTCTTTTTGAAAAGTG
1041 AGTTTATTTTCAGCAAGGCTTTATCTATGGGAATCTTGAGTGTCTGTTTATGTCATATTCCCAGGGCTGTTGCTGCACAC
1121 AAGCCCATTCTTATTTTAATTTCTTGGCTTTAGGGTTTCCATACCTGAAGTGTAGCATAAATACTGATAGGAGATTTCCC
1201 AGGCCAAGGCAAACACACTTCCTCCTCATCTCCTTGTGCTAGTGGGCAGAATATTTGATTGATGCCTTTTTCACTGAGAG
1281 TATAAGCTTCCATGTGTCCCACCTTTATGGCAGGGGTGGAAGGAGGTACATTTAATTCCCACTGCCTGCCTTTGGCAAGC
1361 CCTGGGTTCTTTGCTCCCCATATAGATGTCTAAGCTAAAAGCCGTGGGTTAATGAGACTGGCAAATTGTTCCAGGACAGC
1441 TACAGCATCAGCTCACATATTCACCTCTCTGGTTTTTCATTCCCCTCATTTTTTTCTGAGACAGAGTCTTGCTCTGTCAC
1521 CCAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTGAAACCTCTGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGCC
1601 TCCCGAGTAGCTGGGACTACAGGCGTGTGCCAACACGCCCGGCTAATTTTTTGTATTTTTATTAGAGACGGAGTTTCACC
1681 GTGTTAGCCAGGATGGTCTCGATCGCTTGACCTCGTGATCCACCCTCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGT
1761 GAGCCACCGCGCCCGGCCTCATTCCCCTCATTTTTGACCGTAAGGATTTCCCCTTTCTTGTAAGTTCTGCTATGTATTTA
1841 AAAGAATGTTTTCTACATTTTATCCAGCATTTCTCTGTGTTCTGTTGGAAGGGAAGGGCTTAGGTATCTAGTTTGATACA
1921 TAGGTAGAAGTGGAACATTTCTCTGTCCCCCAGCTGTCATCATATAAGATAAACATCAGATAAAAAGCCACCTGAAAGTA
2001 AAACTACTGACTCGTGTATTAGTGAGTATAATCTCTTCTCCATCCTTAGGAAAATGTTCATCCCAGCTGCGGAGATTAAC
2081 AAATGGGTGATTGAGCTTTCTCCTCGTATTTGGACCTTGAAGGTTATATAAATTTTTTTCTTATGAAGAGTTGGCATTTC
2161 TTTTTATTGCCAATGGCAGGCACTCATTCATATTTGATCTCCTCACCTTCCCCTCCCCTAAAACCAATCTCCAGAACTTT
2241 TTGGACTATAAATTTCTTGGTTTGACTTCTGGAGAACTGTTCAGAATATTACTTTGCATTTCAAATTACAAACTTACCTT
2321 GGTGTATCTTTTTCTTACAAGCTGCCTAAATGAATATTTGGTATATATTGGTAGTTTTATTACTATAGTAAATCAAGGAA
2401 ATGCAGTAAACTTAAAATGTCTTTAAGAAAGCCCTGAAATCTTCATGGGTGAAATTAGAAATTATCAACTAGATAATAGT
2481 ATAGATAAATGAATTTGTAGCTAATTCTTGCTAGTTGTTGCATCCAGAGAGCTTTGAATAACATCATTAATCTACTCTTT
2561 AGCCTTGCATGGTATGCTATGAGGCTCCTGTTCTGTTCAAGTATTCTAATCAATGGCTTTGAAAAGTTTATCAAATTTAC
2641 ATACAGATCACAAGCCTAGGAGAAATAACTAATTCACAGATGACAGAATTAAGATTATAAAAGATTTTTTTTTTGTAATT
2721 TTAGTAGAGACAGGGTTGCCATTGTATTCCAGCCTTGGCGACAGAGCAAGACTCTGCCTCAAAAAAAAAAAAAAAAAGGT
2801 TTTGGCAAGCTGGAACTCTTTCTGCAAATGACTAAGATAGAAAACTGCCAAGGACAAATGAGGAGTAGTTAGATTTTGAA
2881 AATATTAATCATAGAATAGTTGTTGTATGCTAAGTCACTGACCCATATTATGTACAGCATTTCTGATCTTTACTTTGCAA
2961 GATTAGTGATACTATCCCAATACACTGCTGGAGAAATCAGAATTTGGAGAAATAAGTTGTCCAAGGCAAGAAGATAGTAA
3041 ATTATAAGTACAAGTGTAATATGGACAGTATCTAACTTGAAAAGATTTCAGGCGAAAAGAATCTGGGGTTTGCCAGTCAG
3121 TTGCTCAAAAGGTCAATGAAAACCAAATAGTGAAGCTATCAGAGAAGCTAATAAATTATAGACTGCTTGAACAGTTGTGT
3201 CCAGATTAAGGGAGATAATAGCTTTCCCACCCTACTTTGTGCAGGTCATACCTCCCCAAAGTGTTTACCTAATCAGTAGG
3281 TTCACAAACTCTTGGTCATTATAGTATATGCCTAAAATGTATGCACTTAGGAATGCTAAAAATTTAAATATGGTCTAAAG
3361 CAAATAAAAGCAAAGAGGAAAAACTTTGGACAGCGTAAAGACTAGAATAGTCTTTTAAAAAGAAAGCCAGTATATTGGTT
3441 TGAAATATAGAGATGTGTCCCAATTTCAAGTATTTTAATTGCACCTTAATGAAATTATCTATTTTCTATAGATTTTAGTA
3521 CTATTGAATGTATTACTTTACTGTTACCTGAATTTATTATAAAGTGTTTTTGAATAAATAATTCTAAAAGC
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' guGAAUAGUCCAACAUAAUAUu 5'
            |||:||| | |||| |||| 
Target 5' ctCTTGTCA-GATGTA-TATAa 3'
766 - 785 123.00 -12.80
2
miRNA  3' guGAAUAG--UC-CAAC-AUAAUAUu 5'
            :| |||  ||  | | |:||:|| 
Target 5' taTTAATCATAGAATAGTTGTTGTAt 3'
2883 - 2908 114.00 -5.50
3
miRNA  3' guGAAU-AGUCCAACAUAAUauu 5'
            || | ||:   |||||||   
Target 5' taCTGACTCG---TGTATTAgtg 3'
2005 - 2024 113.00 -5.80
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-374a-5p :: ATM    [ Functional MTI ]
Validation Method Immunoblot , Luciferase reporter assay , qRT-PCR , Other
Conditions JHU-029
Location of target site 3'UTR
Tools used in this research microRNA.org
Original Description (Extracted from the article) ... Our study establishes a new functional link between p-DNp63a and the deregulated miRNAome during CIS-induced tumor cell death, suggesting novel mechanisms (transcriptional regulation and DICER1-dependent pri-miRNA maturation) through which p-DNp63a could potentially act as a regulator of death or survival for HNSCC cells during chemotherapy ...

- Huang, Y. Chuang, A. Hao, H. Talbot, C. et al., 2011, Cell death and differentiation.

Article - Huang, Y. Chuang, A. Hao, H. Talbot, C. et al.
- Cell death and differentiation, 2011
Head and neck squamous cell carcinoma (HNSCC) cells exposed to cisplatin (CIS) displayed a dramatic ATM-dependent phosphorylation of DeltaNp63alpha that leads to the transcriptional regulation of downstream mRNAs. Here, we report that phospho (p)-DeltaNp63alpha transcriptionally deregulates miRNA expression after CIS treatment. Several p-DeltaNp63alpha-dependent microRNA species (miRNAs) were deregulated in HNSCC cells upon CIS exposure, including miR-181a, miR-519a, and miR-374a (downregulated) and miR-630 (upregulated). Deregulation of miRNA expression led to subsequent modulation of mRNA expression of several targets (TP53-S46, HIPK2, ATM, CDKN1A and 1B, CASP3, PARP1 and 2, DDIT1 and 4, BCL2 and BCL2L2, TP73, YES1, and YAP1) that are involved in the apoptotic process. Our data support the notion that miRNAs are critical downstream targets of p-DeltaNp63alpha and mediate key pathways implicated in the response of cancer cells to chemotherapeutic drugs.Cell Death and Differentiation advance online publication, 28 January 2011; doi:10.1038/cdd.2010.188.
LinkOut: [PMID: 21274007]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
9 hsa-miR-374a-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004566 EP300 E1A binding protein p300 2 1
MIRT005572 DICER1 dicer 1, ribonuclease type III 3 1
MIRT005573 ATM ataxia telangiectasia mutated 3 1
MIRT005574 GADD45A growth arrest and DNA-damage-inducible, alpha 3 1
MIRT044013 FAM126A family with sequence similarity 126, member A 1 1
MIRT044014 MTF2 metal response element binding transcription factor 2 1 1
MIRT044015 SERBP1 SERPINE1 mRNA binding protein 1 1 1
MIRT044016 HMGB2 high mobility group box 2 1 1
MIRT044017 USP8 ubiquitin specific peptidase 8 1 1