Accession ID: MIRT005573 [miRNA, hsa-miR-374a ::
ATM, target gene]
| pre-miRNA ID | hsa-mir-374a LinkOut: [miRBase ] |
|---|---|
| Description | Homo sapiens miR-374a stem-loop |
| 2nd Structure of pre-miRNA |  |
| Mature miRNA | hsa-miR-374a-3p |
|---|---|
| Mature Sequence | 42| CUUAUCAGAUUGUAUUGUAAUU |63 |
| Evidence | Experimental |
| Experiments | Cloned |
| Putative hsa-miR-374a-3p Targets | LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ] |
| Mature miRNA | hsa-miR-374a-5p |
| Mature Sequence | 12| UUAUAAUACAACCUGAUAAGUG |33 |
| Evidence | Experimental |
| Experiments | Cloned |
| Putative hsa-miR-374a-5p Targets | LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ] |
 |
| Gene Symbol | ATM LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ] | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Synonyms | AT1, ATA, ATC, ATD, ATDC, ATE, DKFZp781A0353, MGC74674, TEL1, TELO1 | ||||||||||||||||||||
| Description | ataxia telangiectasia mutated | ||||||||||||||||||||
| Transcript | NM_000051 LinkOut: [ RefSeq ] | ||||||||||||||||||||
| Other Transcripts | NM_138292 | ||||||||||||||||||||
| Expression | LinkOut: [ BioGPS ] | ||||||||||||||||||||
| KEGG Pathway |
hsa04110 Cell cycle - Homo sapiens (human) hsa04115 p53 signaling pathway - Homo sapiens (human) hsa04210 Apoptosis - Homo sapiens (human) |
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| Putative miRNA Targets on ATM | LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ] | ||||||||||||||||||||
| 3'UTR of ATM (miRNA target sites are highlighted) |
>ATM|NM_000051|3'UTR 1 TGATCTTCAGTATATGAATTACCCTTTCATTCAGCCTTTAGAAATTATATTTTAGCCTTTATTTTTAACCTGCCAACATA 81 CTTTAAGTAGGGATTAATATTTAAGTGAACTATTGTGGGTTTTTTTGAATGTTGGTTTTAATACTTGATTTAATCACCAC 161 TCAAAAATGTTTTGATGGTCTTAAGGAACATCTCTGCTTTCACTCTTTAGAAATAATGGTCATTCGGGCTGGGCGCAGCG 241 GCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGAGCGGATCACAAGGTCAGGAGTTCGAGACCAGCCTGGCCA 321 AGAGACCAGCCTGGCCAGTATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCGAGCATGGTGGCGGGCACCT 401 GTAATCCCAGCTACTCGAGAGGCTGAGGCAGGAGAATCTCTTGAACCTGGGAGGTGAAGGTTGCTGTGGGCCAAAATCAT 481 GCCATTGCACTCCAGCCTGGGTGACAAGAGCGAAACTCCATCTCAAAAAAAAAAAAAAAAAAACAGAAACGTATTTGGAT 561 TTTTCCTAGTAAGATCACTCAGTGTTACTAAATAATGAAGTTGTTATGGAGAACAAATTTCAAAGACACAGTTAGTGTAG 641 TTACTATTTTTTTAAGTGTGTATTAAAACTTCTCATTCTATTCTCTTTATCTTTTAAGCCCTTCTGTACTGTCCATGTAT 721 GTTATCTTTCTGTGATAACTTCATAGATTGCCTTCTAGTTCATGAATTCTCTTGTCAGATGTATATAATCTCTTTTACCC 801 TATCCATTGGGCTTCTTCTTTCAGAAATTGTTTTTCATTTCTAATTATGCATCATTTTTCAGATCTCTGTTTCTTGATGT 881 CATTTTTAATGTTTTTTTAATGTTTTTTATGTCACTAATTATTTTAAATGTCTGTACTTGATAGACACTGTAATAGTTCT 961 ATTAAATTTAGTTCCTGCTGTTTATATCTGTTGATTTTTGTATTTGATAGGCTGTTCATCCAGTTTTGTCTTTTTGAAAA 1041 GTGAGTTTATTTTCAGCAAGGCTTTATCTATGGGAATCTTGAGTGTCTGTTTATGTCATATTCCCAGGGCTGTTGCTGCA 1121 CACAAGCCCATTCTTATTTTAATTTCTTGGCTTTAGGGTTTCCATACCTGAAGTGTAGCATAAATACTGATAGGAGATTT 1201 CCCAGGCCAAGGCAAACACACTTCCTCCTCATCTCCTTGTGCTAGTGGGCAGAATATTTGATTGATGCCTTTTTCACTGA 1281 GAGTATAAGCTTCCATGTGTCCCACCTTTATGGCAGGGGTGGAAGGAGGTACATTTAATTCCCACTGCCTGCCTTTGGCA 1361 AGCCCTGGGTTCTTTGCTCCCCATATAGATGTCTAAGCTAAAAGCCGTGGGTTAATGAGACTGGCAAATTGTTCCAGGAC 1441 AGCTACAGCATCAGCTCACATATTCACCTCTCTGGTTTTTCATTCCCCTCATTTTTTTCTGAGACAGAGTCTTGCTCTGT 1521 CACCCAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTGAAACCTCTGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCA 1601 GCCTCCCGAGTAGCTGGGACTACAGGCGTGTGCCAACACGCCCGGCTAATTTTTTGTATTTTTATTAGAGACGGAGTTTC 1681 ACCGTGTTAGCCAGGATGGTCTCGATCGCTTGACCTCGTGATCCACCCTCCTCGGCCTCCCAAAGTGCTGGGATTACAGG 1761 TGTGAGCCACCGCGCCCGGCCTCATTCCCCTCATTTTTGACCGTAAGGATTTCCCCTTTCTTGTAAGTTCTGCTATGTAT 1841 TTAAAAGAATGTTTTCTACATTTTATCCAGCATTTCTCTGTGTTCTGTTGGAAGGGAAGGGCTTAGGTATCTAGTTTGAT 1921 ACATAGGTAGAAGTGGAACATTTCTCTGTCCCCCAGCTGTCATCATATAAGATAAACATCAGATAAAAAGCCACCTGAAA 2001 GTAAAACTACTGACTCGTGTATTAGTGAGTATAATCTCTTCTCCATCCTTAGGAAAATGTTCATCCCAGCTGCGGAGATT 2081 AACAAATGGGTGATTGAGCTTTCTCCTCGTATTTGGACCTTGAAGGTTATATAAATTTTTTTCTTATGAAGAGTTGGCAT 2161 TTCTTTTTATTGCCAATGGCAGGCACTCATTCATATTTGATCTCCTCACCTTCCCCTCCCCTAAAACCAATCTCCAGAAC 2241 TTTTTGGACTATAAATTTCTTGGTTTGACTTCTGGAGAACTGTTCAGAATATTACTTTGCATTTCAAATTACAAACTTAC 2321 CTTGGTGTATCTTTTTCTTACAAGCTGCCTAAATGAATATTTGGTATATATTGGTAGTTTTATTACTATAGTAAATCAAG 2401 GAAATGCAGTAAACTTAAAATGTCTTTAAGAAAGCCCTGAAATCTTCATGGGTGAAATTAGAAATTATCAACTAGATAAT 2481 AGTATAGATAAATGAATTTGTAGCTAATTCTTGCTAGTTGTTGCATCCAGAGAGCTTTGAATAACATCATTAATCTACTC 2561 TTTAGCCTTGCATGGTATGCTATGAGGCTCCTGTTCTGTTCAAGTATTCTAATCAATGGCTTTGAAAAGTTTATCAAATT 2641 TACATACAGATCACAAGCCTAGGAGAAATAACTAATTCACAGATGACAGAATTAAGATTATAAAAGATTTTTTTTTTGTA 2721 ATTTTAGTAGAGACAGGGTTGCCATTGTATTCCAGCCTTGGCGACAGAGCAAGACTCTGCCTCAAAAAAAAAAAAAAAAA 2801 GGTTTTGGCAAGCTGGAACTCTTTCTGCAAATGACTAAGATAGAAAACTGCCAAGGACAAATGAGGAGTAGTTAGATTTT 2881 GAAAATATTAATCATAGAATAGTTGTTGTATGCTAAGTCACTGACCCATATTATGTACAGCATTTCTGATCTTTACTTTG 2961 CAAGATTAGTGATACTATCCCAATACACTGCTGGAGAAATCAGAATTTGGAGAAATAAGTTGTCCAAGGCAAGAAGATAG 3041 TAAATTATAAGTACAAGTGTAATATGGACAGTATCTAACTTGAAAAGATTTCAGGCGAAAAGAATCTGGGGTTTGCCAGT 3121 CAGTTGCTCAAAAGGTCAATGAAAACCAAATAGTGAAGCTATCAGAGAAGCTAATAAATTATAGACTGCTTGAACAGTTG 3201 TGTCCAGATTAAGGGAGATAATAGCTTTCCCACCCTACTTTGTGCAGGTCATACCTCCCCAAAGTGTTTACCTAATCAGT 3281 AGGTTCACAAACTCTTGGTCATTATAGTATATGCCTAAAATGTATGCACTTAGGAATGCTAAAAATTTAAATATGGTCTA 3361 AAGCAAATAAAAGCAAAGAGGAAAAACTTTGGACAGCGTAAAGACTAGAATAGTCTTTTAAAAAGAAAGCCAGTATATTG 3441 GTTTGAAATATAGAGATGTGTCCCAATTTCAAGTATTTTAATTGCACCTTAATGAAATTATCTATTTTCTATAGATTTTA 3521 GTACTATTGAATGTATTACTTTACTGTTACCTGAATTTATTATAAAGTGTTTTTGAATAAATAATTCTAAAAGC Target sites Provided by authors Predicted by miRanda |
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| miRNA-target interactions (Predicted by miRanda) |
|
| miRNA:Target | hsa-miR-374a :: ATM [ Functional MTI ] |
|---|---|
| Validation Method | Immunoblot , Luciferase reporter assay , qRT-PCR |
| Conditions | JHU-029 |
| Location of target site | 3'UTR |
| Tools used in this research | microRNA.org |
| Original Description (Extracted from the article) | ... Our study establishes a new functional link between p-DNp63a and the deregulated miRNAome during CIS-induced tumor cell death, suggesting novel mechanisms (transcriptional regulation and DICER1-dependent pri-miRNA maturation) through which p-DNp63a could potentially act as a regulator of death or survival for HNSCC cells during chemotherapy ... - Huang, Y. Chuang, A. Hao, H. Talbot, C. et al., 2011, Cell death and differentiation. |
| Article |
- Huang, Y.
Chuang, A.
Hao, H.
Talbot, C. et al. - Cell death and differentiation, 2011
Head and neck squamous cell carcinoma (HNSCC) cells exposed to cisplatin (CIS) displayed a dramatic ATM-dependent phosphorylation of DeltaNp63alpha that leads to the transcriptional regulation of downstream mRNAs. Here, we report that phospho (p)-DeltaNp63alpha transcriptionally deregulates miRNA expression after CIS treatment. Several p-DeltaNp63alpha-dependent microRNA species (miRNAs) were deregulated in HNSCC cells upon CIS exposure, including miR-181a, miR-519a, and miR-374a (downregulated) and miR-630 (upregulated). Deregulation of miRNA expression led to subsequent modulation of mRNA expression of several targets (TP53-S46, HIPK2, ATM, CDKN1A and 1B, CASP3, PARP1 and 2, DDIT1 and 4, BCL2 and BCL2L2, TP73, YES1, and YAP1) that are involved in the apoptotic process. Our data support the notion that miRNAs are critical downstream targets of p-DeltaNp63alpha and mediate key pathways implicated in the response of cancer cells to chemotherapeutic drugs.Cell Death and Differentiation advance online publication, 28 January 2011; doi:10.1038/cdd.2010.188.
LinkOut: [PMID: 21274007]
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