Accession ID: MIRT005578 [miRNA, hsa-miR-630 :: BCL2, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-630LinkOut: [miRBase ]
Synonyms MIRN630, hsa-mir-630, MIR630
Description Homo sapiens miR-630 stem-loop
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-630
Mature Sequence 61| AGUAUUCUGUACCAGGGAAGGU |82
Evidence Experimental
Experiments RT-PCR
Putative hsa-miR-630 Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms
Description
Transcript    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of
(miRNA target sites are highlighted)
>||3'UTR
Target sites Provided by authors  Predicted by miRanda
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-630 :: BCL2    [ Functional MTI ]
Validation Method Immunoblot , Luciferase reporter assay , qRT-PCR , Other
Conditions JHU-029
Location of target site 3'UTR
Tools used in this research microRNA.org
Original Description (Extracted from the article) ... Our study establishes a new functional link between p-DNp63a and the deregulated miRNAome during CIS-induced tumor cell death, suggesting novel mechanisms (transcriptional regulation and DICER1-dependent pri-miRNA maturation) through which p-DNp63a could potentially act as a regulator of death or survival for HNSCC cells during chemotherapy ...

- Huang, Y. Chuang, A. Hao, H. Talbot, C. et al., 2011, Cell death and differentiation.

Article - Huang, Y. Chuang, A. Hao, H. Talbot, C. et al.
- Cell death and differentiation, 2011
Head and neck squamous cell carcinoma (HNSCC) cells exposed to cisplatin (CIS) displayed a dramatic ATM-dependent phosphorylation of DeltaNp63alpha that leads to the transcriptional regulation of downstream mRNAs. Here, we report that phospho (p)-DeltaNp63alpha transcriptionally deregulates miRNA expression after CIS treatment. Several p-DeltaNp63alpha-dependent microRNA species (miRNAs) were deregulated in HNSCC cells upon CIS exposure, including miR-181a, miR-519a, and miR-374a (downregulated) and miR-630 (upregulated). Deregulation of miRNA expression led to subsequent modulation of mRNA expression of several targets (TP53-S46, HIPK2, ATM, CDKN1A and 1B, CASP3, PARP1 and 2, DDIT1 and 4, BCL2 and BCL2L2, TP73, YES1, and YAP1) that are involved in the apoptotic process. Our data support the notion that miRNAs are critical downstream targets of p-DeltaNp63alpha and mediate key pathways implicated in the response of cancer cells to chemotherapeutic drugs.Cell Death and Differentiation advance online publication, 28 January 2011; doi:10.1038/cdd.2010.188.
LinkOut: [PMID: 21274007]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
4 hsa-miR-630 Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT005578 BCL2 B-cell CLL/lymphoma 2 3 1
MIRT005579 BCL2L2 BCL2-like 2 3 1
MIRT005580 YAP1 Yes-associated protein 1 3 1
MIRT007241 SNAI2 snail homolog 2 (Drosophila) 1 2