Accession ID: MIRT005595 [miRNA, hsa-miR-181b ::
CYLD, target gene]
| miRNA name | hsa-miR-181b |
|---|
 |
| Gene Symbol | CYLD LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ] |
|---|---|
| Synonyms | CDMT, CYLD1, CYLDI, EAC, FLJ20180, FLJ31664, FLJ78684, KIAA0849, MFT, MFT1, SBS, TEM, USPL2 |
| Description | cylindromatosis (turban tumor syndrome) |
| Transcript | NM_001042355 LinkOut: [ RefSeq ] |
| Other Transcripts | NM_001042412, NM_015247 |
| Expression | LinkOut: [ BioGPS ] |
| KEGG Pathway |
hsa04622 RIG-I-like receptor signaling pathway - Homo sapiens (human) |
| Putative miRNA Targets on CYLD | LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ] |
| 3'UTR of CYLD (miRNA target sites are highlighted) |
>CYLD|NM_001042355|3'UTR 1 TAACTGGGGTCATCGGGAAAGGCAAAGAAACTGAAGGCAGAGTCCTAACGTTGCATCTTATTCGAGCTGGCAGTTCTGTT 81 CACGTCCATTGCCGGCAATGGATGTCTTTGTGGTGATGATCCTTCAGAAAAGGATGCCTCTGTTTAAAAACAAATTGCTT 161 TTGTGTCCCTGAAGTATTTAATAAGAAGCATTTTGCACTCTAGAAAGTATGTTTGTGTTGGTTTTTTAAGAAGTCTAAAT 241 GAAGTTATTAATACCTGAAGCTTTAAGTTAAGTGCATTGATCATATGATATTTTTGGAAGCATACAATTTTAATTGTGGA 321 AGTTTAAAGCCTCTTTTAGTCCATTGAGAATGTAAATAAATGTGTCTTCTTTATGGACCAAGGATATGAAATCATTTTTC 401 TTTTGTAGCTAACGGTTGCCTTGAGGAAGAAATAATTTGGTTTTATTAAGAGTCTACTCTCAATCCAGTTATTAGAGATG 481 TACTGAGTTTGATTTGTTAATCCTTTCTATATACTGCTGATCTTGCATGTCTACAATCTGCTCAGTTTTTCTGTGTTTCT 561 GCAATAGTGGTCAGAAAAATACTTAAATTCCCTTAATGGTGTTGTTTTCTATTTGTTCTGGTTTTGAGATAAATGAGTGA 641 TTCTGTCCCCAAATGTCCATTTTTGAAGTGATTTTCCTGGAGGATTAGGGTATTTAGCAGTTGAAGCTCTTCATTCATAG 721 TAGTTACTGTCAGCTAACAGGTTTTTTAAGGCTTTTAACTATTAATATTTTATGGAATGGGGCAAAGTAAATTGATGAAA 801 GAATTGGAGTGATAATAGTCCTTTACAAACATACAGTCCATAAGAAAATGAATTTGGCATATAGAATTATTACAATTTCC 881 TGGGAGAGATGGATATTTAAACCTCTATTATTTTAGACAAGACTGTCTAGAACTTAAGTTTGATCTGTCAGCCAGTACTC 961 CCATTAAATTCAGTGTAGTTTCACTTGATAGAATCAGATATGTTATCGAAATGTTAGCAGCAGCTTCATCCTCCTTCTGA 1041 TTAAAGTAAGTAGAAATGGGATGTTTTGTTTAATAACAGCCATAGTGTGTGTTTAGACCACAGCGGATGTTGTAGACCAG 1121 GACCATAGATGATACATGTCAGTGCTGTGGAATGTGCATTCTCTGAGTGTTGTTTTGTGGTATCATTGTCTTTCCTGAAT 1201 GACTTTCTAACTGTGCAGAAAGGCAGAAAAGTCATCATATGTATATGTCATATGACTTTATAAAATATTTAATGTGACAA 1281 AAAGTGGAAAGAATCTTTACAAACCCTGCAATTACTTTTTTAAAGGCACTTTTACTCTTTGGTTTTATCATTCCATTTTG 1361 CTAATATTTACTAGCTTTATAAATTACAGTAAGGTACAAAAACTCATCTTGTAATATTTTCATTTTTGAAGTGAAAAAGT 1441 ACATATATTTTGCACAAGGTTTTATACTGCTAAGTGCTTGGTTGGGGTGGTGAGATGATGATTAGATCAGGGGTGAGGCT 1521 GAGAGACTCTGGGTTTAGGGCTAGCCCTGCCTCCATCTCCCTTGGGTAAAATGAAGGGTGTGGGGTAAAAGATGCATAAG 1601 GCCTTTTCTAGCTCTGACAGCCTAGAAGTCCAATCACCCTGTAATAAATATGTGTTGAATGAAGAAATGGGTGAATGAGC 1681 TTGTCAATGTGATTTTAAAAAATTGACTACCTGGAGGAATGATTAGGAATCTAAATGAAGCCAGCCCTCGGTATCTGCAG 1761 GTTTCTCATCCATGGATTCAACCAACTGCAAATGGAAAATACGATTTTTTTTAAAAAAAGGATGGTTACATCCGTATTGA 1841 ACATGTACAGACTTTTTTCTTGTCATTATTCTCTGAACAATACAAGAACTCTTTATGTAGCATTTACATTTATTAGGTAT 1921 TATAAGTAATCTAGAGATTATTTAATTAAAATATACAGGAGGATGTGTGTCAGTTATATGCAAATTCTGTACCATTTTGT 2001 ATCAGGGAATTGAGCATCTTCAGATGTTGGTATCTGCAGGGATCCTGGAACCAAACCCCTGCAGATACTAAGGGCTGACG 2081 ATCTAGGTAAGACTGGATTTAACAGTTGGAAAAAAAAAAAAAAAAGGAGAGAGAAGACAGTTCCTTTCCTGTAGAAATTA 2161 AAACAAAATACAAATTGAGGAAGCTCTGCTACCCAGGCTGTCATGGTAGAGAACTTGAAGAAGACCTGTTTGGATGGACA 2241 CCTGGTTTCAAAAGTCAGGTGTGGAGACTGTTAAATGGGAGGGCCTCATCCATAAATGATTTCTGGCAACGTCTTCTTCA 2321 GGTGGAGCTTGACGTCTTTTTAATGTTACTTGGGGAGGGAGTGCTCATTAAGGGATGCCAGGGCCAGCTCTGGTGGTTCC 2401 TGGGGAGGCTGCGTCCTTCCCTGCTTCTGCATGTCATGAGGCAGCAGGAAGGTTTCCCCTGCACCTGTCTGTCCTGGCTC 2481 CCTCTGGGTAGCCCCCTACTGTTCTGTGCTTCAGCACAGCCTGGTTTGTCAAGAGGCACATAGTTGGGGCTGGGCTGCAT 2561 GGCACAGGGGCTTATGTGCCTGCTGGTTATTTAATTTTCAGCCTTAAGTTTTCTTTAATATTTTCCTGTTGGCTATTTAA 2641 AGGTTTTGGTTATCTTTTATTCCTTATCTACAATCAAGATGACAATGTAATTGAATTATCTTATTTATAACACGGTTCGT 2721 GATTCATGATTCATGATTACAAGTAGAAAATATGTCATGTTCCTCACCTCCAAATAAATATGTGTGTGTCTGTGTGTGTG 2801 TATATATGTATGTGGCGGAGAGGGAGAGAGTGGGGAAGGAGAGCAGTGTTATCATACATAGAGAGGCTAAATGTGTCCCA 2881 TCCCTCACTGTCAGCTTTATAAAGGAGTTTGACTCCATCCACAGAAGAATGTTTTATAAGACTAGGAAAACACGTTGAAA 2961 ACTAGGATAAACAGCAACAAAAATCAACTAAATATGTTGTTACTGTTGCTAAGGATTTTCTCCTTAGAATAATTTAGGAT 3041 TTTTAAAAATTTCTGTTTGCCAAATGCTGTAGATAAATGGCCAGATTCTTCCTATCCCTAGGATTCCTTTATTATTTTTT 3121 TTCACAGATTTTGAGAACAAGGGGGAGAGATAGTATGGAAGATTAAGATTCCATTAATCTTATAGAACTGTGTTGTCACC 3201 CAAATTCCTGCTTGTTTGAACATGGCATCTTCATAGATTCAGGATTCACTACCCTCTATAGCTGGATCTTGAAAATTATC 3281 TGGCCAGATAATTTTGCATCTGCTTGGATGATTGTAGACTGAGATGTGAGTGGAGGATAAAGTATTAGACTTTTGCTGAG 3361 TAACTGCCAACCAAGAAGTATTTATCGGACACTTACTAGGTGCCTAGGATTGTATCAGAGGGAATATGAAATGTGTCCCT 3441 GCCCTACCTAGTTTTAACGACAGAATATCTATTAAAGGCTACTTAGCTGAAGGGTAAGGGTGACAGGTCTAGGGGAAGCT 3521 TTGGGAGGTGGTGTGCTGTGACAGAAAAAGTGGCAGAGTAGGGACGAGAGACCTGCATTCTAGCCCTGTTTCTGTCACTT 3601 GCTCTGTACACTTAGACAACAGCTTGACCTCTTGAGCTTTAGTTTCCTCCTCTGCATAATGAGAGGGTTAGACTACTGAA 3681 TTGTATGGGAAAAAAATACAAATTCCTGGGTCCTAGGCCATGCCTGCTGAATCCGACTGTTCAGGAAGAGGCCTAGGAAA 3761 TCTGTGAGGGAATCCCCAGGGGAATCTCGTGACCAGCCAGGTGTGAAATCTGCTAACTGGAAGATCTCAAAGCTTCCTTC 3841 ACTTTTTGTGATTTTGTGGTCATGTAACGTTACTGTATTATTCTACGTAAATGTGGGTACTTGGATGTTTATCATACTGT 3921 TTCTCTGTGTTTACATACTAATTTGTGTAAGAAATGCATTTTAGTCTGTGTACCTCAACCTGCTGTTTGTTTCCTAGAGG 4001 TGTTAGTAGTCTTTAAATACAAGTAAGACTTAAGAGGATATTTGATGTTATTTACCTGGATATTTTCTTCCCCTTTTATT 4081 TATTTAGAGGAAATTGAGATTCTAGGAGCCAAAAAAATGAAAACAAAATTCTAAGGCAAAGTTAAAGAAAAAAATTACAT 4161 TATTTCTTACCATTTGCTACTTTATAATGAAAATTTAAAAATTATATGGGAAGATTTTTCTCTGGGATAACAAATCCTTG 4241 TCATAAAGTAAGAGGTCTTTTTAAAGTAGGTAGGCTATAAGGCCTGTAATTTAAAATAATACTCCTTTCTCTAGGGTTTG 4321 GTGCAATTCTCCATTAATGAAGATAACATTTGAATTCCCCAAAGCAGGTGAGGAGTCGGGGAGGAGAAAGCGATGTTAAA 4401 ATGAAAACTCACTGCAAAAGAGGAGGCAGAGGAAGAAGGAATGTAAACCCCTTAAAGCAGATGTGTGTGGGGCCTTATGA 4481 AGACCAGGATTCTGCGGGTGTCAGGGGATTGCCCCTCTTGACAGAGACTAGGGTTTTAGACTGAGGCTTCCTGCAGGGTG 4561 TTCGCATTGCCCTTCTCCGTTCCCCTTCAGACCTTTCTGGGGAGAAGAGGTGGGAGGAGGGGAGAAAGACTGTTCATCTT 4641 ATTCTGAATCCTGGAGCAGCTGAAGGTTTTCTCTTGAGTCAGGATGCAGTGGTAATGCATTAACCAGCAAGTGTGGCCAA 4721 GGATAATGAAAAAGTGGGAAAGGAAGGTCCTCCTCCTCCCTGATTGTAGCATCCAGCAGTCTCTGTAGCCAGGTTACTCA 4801 AGAACCACATTTGATTTCCTGGCCCTTTGCCTTGGCAGTGATGGCATTTTTATTTCACTGTGTTTTAAAGTCTTCATTTA 4881 TTTTTATAACATGGGTTAGGGAGAAGGGCCACAAATGGAGGGATTGTCCTTTCAAGCACCACAGCTTCAGATAAAATTAG 4961 TACTTTCAAATATTGTCCACTTTAACTTAAAAAATTCTAGAGGGATTATATTGGAGACTCAACTGCCCTTGGTTTTAGTT 5041 TATAAAATGGCCTAGTACTGTGGAATTTTAATTTTAGAAAGTCTTAGCATCAGATCATAAACATTCATTAAAAGAACTCA 5121 CATCCCATCTGAAACTTCCCAGGGGAGTTGGGATTCTTAGTAGATTGGTAGAAAGGGGCTCATTTTCTACTGCATTTCCC 5201 ATTTTTGGTATCTTGTTCAGCATGTTTTATTTTTATTTCTTGTCTGCAGAACATCCTATATTTATGAGAACATTCTTTAA 5281 GAAGACCACCACATAGAATACCCCTTCCTATCAGCTCGCTCTGATTTAGCCTTAATTTTGTTAAATTTTTTAGAGATGAA 5361 TGAAGTGCTGCTGTGGAAAGAAATGTACATATACTATTTCTGTATCATTAAAATTACATTTTTATGGTTCAAAAAAAAAA 5441 AAAAAAA Target sites Provided by authors Predicted by miRanda |
| miRNA:Target | hsa-miR-181b :: CYLD [ Functional MTI ] | ||||||
|---|---|---|---|---|---|---|---|
| Validation Method | Luciferase reporter assay , qRT-PCR | ||||||
| Conditions | MCF10A, HCT116, HT29, PC3, A549, HeLa, Hep 3B, PANC1 | ||||||
| Location of target site | 3'UTR | ||||||
| Tools used in this research | Unspecified | ||||||
| Original Description (Extracted from the article) | ... miR-181b-1 regulates directly CYLD expression during transformation// miR-21 and miR-181b-1,respectively, inhibit PTEN and CYLD tumor suppressors, leading to increased NF-kB activity required to maintain the transformed state ... - Iliopoulos, D. Jaeger, S. A. Hirsch, H. A. et al., 2010, Mol Cell. |
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| miRNA-target interactions (Provided by authors) |
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| Article |
- Iliopoulos, D.
Jaeger, S. A.
Hirsch, H. A. et al. - Mol Cell, 2010
A transient inflammatory signal can initiate an epigenetic switch from nontransformed to cancer cells via a positive feedback loop involving NF-kappaB, Lin28, let-7, and IL-6. We identify differentially regulated microRNAs important for this switch and putative transcription factor binding sites in their promoters. STAT3, a transcription factor activated by IL-6, directly activates miR-21 and miR-181b-1. Remarkably, transient expression of either microRNA induces the epigenetic switch. MiR-21 and miR-181b-1, respectively, inhibit PTEN and CYLD tumor suppressors, leading to increased NF-kappaB activity required to maintain the transformed state. These STAT3-mediated regulatory circuits are required for the transformed state in diverse cell lines and tumor growth in xenografts, and their transcriptional signatures are observed in colon adenocarcinomas. Thus, STAT3 is not only a downstream target of IL-6 but, with miR-21, miR-181b-1, PTEN, and CYLD, is part of the positive feedback loop that underlies the epigenetic switch that links inflammation to cancer.
LinkOut: [PMID: 20797623]
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