Accession ID: MIRT005705 [miRNA, hsa-miR-146a :: CDKN3, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-146a LinkOut: [miRBase ]
Synonyms MIRN146, MIRN146A, miR-146a, miRNA146A, MIR146A
Description Homo sapiens miR-146a stem-loop
Comment This miRNA sequence is predicted based on homology to a verified miRNA from mouse .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-146a-3p
Mature Sequence 57| CCUCUGAAAUUCAGUUCUUCAG |78
Evidence Experimental
Experiments Cloned
Putative hsa-miR-146a-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-146a-5p
Mature Sequence 21| UGAGAACUGAAUUCCAUGGGUU |42
Evidence Experimental
Experiments Cloned
Putative hsa-miR-146a-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol CDKN3 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms CDI1, CIP2, FLJ25787, KAP, KAP1, MGC70625
Description cyclin-dependent kinase inhibitor 3
Transcript NM_001130851   LinkOut: [ RefSeq ]
Other Transcripts NM_005192   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on CDKN3 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of CDKN3
(miRNA target sites are highlighted)		 
>CDKN3|NM_001130851|3'UTR
   1 TCATCAAGAGATTCACAATCAAGATCTGTATCAAGATAAAGGAATTCAAATAGCATATATATGACCATGTCTGAAATGTC
  81 AGTTCTCTAGCATAATTTGTATTGAAATGAAACCACCAGTGTTATCAACTTGAATGTAAATGTACATGTGCAGATATTCC
 161 TAAAGTTTTATTGACAAAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' gaCUU-C-UUGAC-U-UA-AAGUCUcc 5'
            ||| | || || | || | ||||  
Target 5' ttGAATGTAAATGTACATGTGCAGAta 3'
 		130 - 156 100.00 -7.20
2 
miRNA  3' gacuucuuGAC-UUAAAGUCucc 5'
                  ||| ||| ||||   
Target 5' gaccatgtCTGAAATGTCAGttc 3'
 		63 - 85 90.00 -8.12
3 
miRNA  3' uugGGUAC---C-UUA-AGUCAAGAGu 5'
             |||||   | ||| ||||||||| 
Target 5' tgaCCATGTCTGAAATGTCAGTTCTCt 3'
 		62 - 88 167.00 -18.90
Experimental Support 1 for Non-Functional miRNA-Target Interaction
miRNA:Target hsa-miR-146a :: CDKN3    [ Non-Functional MTI ]
Validation Method Western blot
Conditions HMVEC
Original Description (Extracted from the article) ... Of the 29 genes, 5 were human genes (CDKN3, CCNA2, PA2G4, KIF22, and BRCA1) belonging to the functional category of GOTERM_BP_ALL: cell cycle process. Mea- surement of the transcripts of these 5 cell cycle-related genes revealed that 3 genes (CCNA2, PA2G4, and BRCA1) were down- regulated after PMA treatment; however, their expression was rescued upon miR-146a inhibition (Fig. 2B). ...

- Hsieh, C. H. Rau, C. S. Jeng, S. F. Lin, C. et al., 2010, Exp Cell Res.
Article - Hsieh, C. H. Rau, C. S. Jeng, S. F. Lin, C. et al.
- Exp Cell Res, 2010
Phorbol 12-myristate 13-acetate (PMA) is known to activate protein kinase C (PKC) and increase angiogenesis in cultured endothelial cells. Using a microRNA (miRNA) array, we found that PMA induced miR-146a expression in human microvascular endothelial cells. The miR-146a expression was dependent on dose and time and independent of PKC activation. Using a combined approach involving predictions using miRanda algorithm and whole genome microarray experiments with or without inhibition of miR-146a expression by LNA-antimir-146a or LNA-control, 29 potential target genes of miR-146a were identified. Because endothelial cell S phase progression is an early event in the induction of angiogenesis, we evaluated 5 cell cycle-related genes from the 29 target genes and found that the transcripts of 3 genes (CCNA2, PA2G4, and BRCA1) were downregulated after PMA treatment, but their expression was rescued upon miR-146a inhibition. However, inhibition of miR-146a expression failed to alter the cell cycle distribution or angiogenesis induced by PMA treatment. By using a combined approach involving computational prediction and a whole genome microarray experiment in the presence or absence of antimir, the observations in this presented article raise the possibility that antimir strategies might be used to identify the potential miRNA targets.
LinkOut: [PMID: 19944095]