Accession ID: MIRT005740 [miRNA, hsa-miR-451a :: AKT1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-451aLinkOut: [miRBase ]
Description Homo sapiens miR-451a stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-451a
Mature Sequence 17| AAACCGUUACCAUUACUGAGUU |38
Evidence Experimental
Experiments Cloned
Putative hsa-miR-451a Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol AKT1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Description v-akt murine thymoma viral oncogene homolog 1
Transcript NM_0051    LinkOut: [ RefSeq ]
Other Transcripts NM_0010144 , NM_0010144   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on AKT1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of AKT1
(miRNA target sites are highlighted)
>AKT1|NM_0051|3'UTR
   1 TCGGCCAGCGGCACGGCCTGAGGCGGCGGTGGACTGCGCTGGACGATAGCTTGGAGGGATGGAGAGGCGGCCTCGTGCCA
  81 TGATCTGTATTTAATGGTTTTTATTTCTCGGGTGCATTTGAGAGAAGCCACGCTGTCCTCTCGAGCCCAGATGGAAAGAC
 161 GTTTTTGTGCTGTGGGCAGCACCCTCCCCCGCAGCGGGGTAGGGAAGAAAACTATCCTGCGGGTTTTAATTTATTTCATC
 241 CAGTTTGTTCTCCGGGTGTGGCCTCAGCCCTCAGAACAATCCGATTCACGTAGGGAAATGTTAAGGACTTCTGCAGCTAT
 321 GCGCAATGTGGCATTGGGGGGCCGGGCAGGTCCTGCCCATGTGTCCCCTCACTCTGTCAGCCAGCCGCCCTGGGCTGTCT
 401 GTCACCAGCTATCTGTCATCTCTCTGGGGCCCTGGGCCTCAGTTCAACCTGGTGGCACCAGATGCAACCTCACTATGGTA
 481 TGCTGGCCAGCACCCTCTCCTGGGGGTGGCAGGCACACAGCAGCCCCCCAGCACTAAGGCCGTGTCTCTGAGGACGTCAT
 561 CGGAGGCTGGGCCCCTGGGATGGGACCAGGGATGGGGGATGGGCCAGGGTTTACCCAGTGGGACAGAGGAGCAAGGTTTA
 641 AATTTGTTATTGTGTATTATGTTGTTCAAATGCATTTTGGGGGTTTTTAATCTTTGTGACAGGAAAGCCCTCCCCCTTCC
 721 CCTTCTGTGTCACAGTTCTTGGTGACTGTCCCACCGGGAGCCTCCCCCTCAGATGATCTCTCCACGGTAGCACTTGACCT
 801 TTTCGACGCTTAACCTTTCCGCTGTCGCCCCAGGCCCTCCCTGACTCCCTGTGGGGGTGGCCATCCCTGGGCCCCTCCAC
 881 GCCTCCTGGCCAGACGCTGCCGCTGCCGCTGCACCACGGCGTTTTTTTACAACATTCAACTTTAGTATTTTTACTATTAT
 961 AATATAATATGGAACCTTCCCTCCAAATTCTTCAATAAAAGTTGCTTTTCAAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-451a :: AKT1    [ Functional MTI ]
Validation Method qRT-PCR , Western blot
Conditions A172 , LN229 , U251
Location of target site 3'UTR
Tools used in this research unknown
Original Description (Extracted from the article) ... We demonstrate that over-expression of miR-451 in human glioblastoma cell lines A172, LN229 and U251 significantly impacts cell proliferation, invasion and apoptosis. Our results suggest that these biologic effects may be due to modulated expression of a panel of proteins including Cyclin D1, p27, MMP 2/9 and Bcl-2. In addition, miR-451 regulates Akt expression in our studies. The transcription factor Akt1 plays a critical role in controlling a range of diverse cellular functions ...

- Nan, Y. Han, L. Zhang, A. Wang, G. Jia, Z. et al., 2010, Brain Res.

Article - Nan, Y. Han, L. Zhang, A. Wang, G. Jia, Z. et al.
- Brain Res, 2010
MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at the post-transcriptional and/or translational level by binding loosely complimentary sequences in the 3'untranslated regions (UTRs) of target mRNAs. Increased expression of several miRNAs, specifically hsa-miR-21, have been reported to modulate glioma development. Here we report downregulation of miR-451 in A172, LN229 and U251 human glioblastoma cells. Increased expression of miR-451 by administration of miR-451 mimics oligonucleotides reversed the biology of each of the three cell lines, inhibiting cell growth, inducing G0/G1 phase arrest and increasing cell apoptosis. Further, treatment with miR-451 mimics oligonucleotides diminished the invasive capacity of these cells, as the number of cells invading through matrigel was significantly decreased. Akt1, CyclinD1, MMP-2, MMP-9 and Bcl-2 protein expression decreased, and p27 expression increased in a dose-dependent manner with miR-451 mimics oligonucleotides. Taken together, these studies reveal miR-451 impacts glioblastoma cell proliferation, invasion and apoptosis, perhaps via regulation of the PI3K/AKT signaling pathway. We propose an essential role for miR-451 as a tumor-suppressor of human glioma.
LinkOut: [PMID: 20816946]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
12 hsa-miR-451a Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000046 MIF macrophage migration inhibitory factor (glycosylation-inhibiting factor) 5 2
MIRT000500 CAB39 calcium binding protein 39 5 2
MIRT005656 ABCB1 ATP-binding cassette, sub-family B (MDR/TAP), member 1 4 1
MIRT005740 AKT1 v-akt murine thymoma viral oncogene homolog 1 2 1
MIRT005742 MMP2 matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) 2 1
MIRT005743 MMP9 matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) 2 1
MIRT005744 BCL2 B-cell CLL/lymphoma 2 2 1
MIRT006358 MYC v-myc myelocytomatosis viral oncogene homolog (avian) 1 1
MIRT006533 RAB14 RAB14, member RAS oncogene family 4 1
MIRT006597 TMED7 transmembrane emp24 protein transport domain containing 7 2 1
MIRT016628 ARPP19 cAMP-regulated phosphoprotein, 19kDa 1 1
MIRT016629 UBE2H ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) 1 1