Accession ID: MIRT005785 [miRNA, hsa-miR-221 :: TP53, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-221 LinkOut: [miRBase ]
Synonyms MIRN221, miRNA221, mir-221, MIR221
Description Homo sapiens miR-221 stem-loop
Comment This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-221-3p
Mature Sequence 65| AGCUACAUUGUCUGCUGGGUUUC |87
Evidence Experimental
Experiments Cloned
Putative hsa-miR-221-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-221-5p
Mature Sequence 25| ACCUGGCAUACAAUGUAGAUUU |46
Evidence Experimental
Experiments Cloned
Putative hsa-miR-221-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol TP53 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms FLJ92943, LFS1, TRP53, p53
Description tumor protein p53
Transcript NM_001126114   LinkOut: [ RefSeq ]
Other Transcripts NM_001126112, NM_000546, NM_001126113, NM_001126115, NM_001126116, NM_001126117   
Expression LinkOut: [ BioGPS ]
KEGG Pathway hsa04010    MAPK signaling pathway - Homo sapiens (human)
hsa04110    Cell cycle - Homo sapiens (human)
hsa04115    p53 signaling pathway - Homo sapiens (human)
hsa04210    Apoptosis - Homo sapiens (human)
hsa04310    Wnt signaling pathway - Homo sapiens (human)
hsa04722    Neurotrophin signaling pathway - Homo sapiens (human)
hsa05014    Amyotrophic lateral sclerosis (ALS) - Homo sapiens (human)
hsa05016    Huntington's disease - Homo sapiens (human)
hsa05200    Pathways in cancer - Homo sapiens (human)
hsa05210    Colorectal cancer - Homo sapiens (human)
hsa05212    Pancreatic cancer - Homo sapiens (human)
hsa05213    Endometrial cancer - Homo sapiens (human)
hsa05214    Glioma - Homo sapiens (human)
hsa05215    Prostate cancer - Homo sapiens (human)
hsa05216    Thyroid cancer - Homo sapiens (human)
hsa05217    Basal cell carcinoma - Homo sapiens (human)
hsa05218    Melanoma - Homo sapiens (human)
hsa05219    Bladder cancer - Homo sapiens (human)
hsa05220    Chronic myeloid leukemia - Homo sapiens (human)
hsa05222    Small cell lung cancer - Homo sapiens (human)
hsa05223    Non-small cell lung cancer - Homo sapiens (human)
Putative miRNA Targets on TP53 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of TP53
(miRNA target sites are highlighted)		 
>TP53|NM_001126114|3'UTR
   1 TAAAGAGAGCATGAAAATGGTTCTATGACTTTGCCTGATACAGATGCTACTTGACTTACGATGGTGTTACTTCCTGATAA
  81 ACTCGTCGTAAGTTGAAAATATTATCCGTGGGCGTGAGCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTC
 161 AAGGATGCCCAGGCTGGGAAGGAGCCAGGGGGGAGCAGGGCTCACTCCAGCCACCTGAAGTCCAAAAAGGGTCAGTCTAC
 241 CTCCCGCCATAAAAAACTCATGTTCAAGACAGAAGGGCCTGACTCAGACTGACATTCTCCACTTCTTGTTCCCCACTGAC
 321 AGCCTCCCACCCCCATCTCTCCCTCCCCTGCCATTTTGGGTTTTGGGTCTTTGAACCCTTGCTTGCAATAGGTGTGCGTC
 401 AGAAGCACCCAGGACTTCCATTTGCTTTGTCCCGGGGCTCCACTGAACAAGTTGGCCTGCACTGGTGTTTTGTTGTGGGG
 481 AGGAGGATGGGGAGTAGGACATACCAGCTTAGATTTTAAGGTTTTTACTGTGAGGGATGTTTGGGAGATGTAAGAAATGT
 561 TCTTGCAGTTAAGGGTTAGTTTACAATCAGCCACATTCTAGGTAGGGGCCCACTTCACCGTACTAACCAGGGAAGCTGTC
 641 CCTCACTGTTGAATTTTCTCTAACTTCAAGGCCCATATCTGTGAAATGCTGGCATTTGCACCTACCTCACAGAGTGCATT
 721 GTGAGGGTTAATGAAATAATGTACATCTGGCCTTGAAACCACCTTTTATTACATGGGGTCTAGAACTTGACCCCCTTGAG
 801 GGTGCTTGTTCCCTCTCCCTGTTGGTCGGTGGGTTGGTAGTTTCTACAGTTGGGCAGCTGGTTAGGTAGAGGGAGTTGTC
 881 AAGTCTCTGCTGGCCCAGCCAAACCCTGTCTGACAACCTCTTGGTGAACCTTAGTACCTAAAAGGAAATCTCACCCCATC
 961 CCACACCCTGGAGGATTTCATCTCTTGTATATGATGATCTGGATCCACCAAGACTTGTTTTATGCTCAGGGTCAATTTCT
1041 TTTTTCTTTTTTTTTTTTTTTTTTCTTTTTCTTTGAGACTGGGTCTCGCTTTGTTGCCCAGGCTGGAGTGGAGTGGCGTG
1121 ATCTTGGCTTACTGCAGCCTTTGCCTCCCCGGCTCGAGCAGTCCTGCCTCAGCCTCCGGAGTAGCTGGGACCACAGGTTC
1201 ATGCCACCATGGCCAGCCAACTTTTGCATGTTTTGTAGAGATGGGGTCTCACAGTGTTGCCCAGGCTGGTCTCAAACTCC
1281 TGGGCTCAGGCGATCCACCTGTCTCAGCCTCCCAGAGTGCTGGGATTACAATTGTGAGCCACCACGTCCAGCTGGAAGGG
1361 TCAACATCTTTTACATTCTGCAAGCACATCTGCATTTTCACCCCACCCTTCCCCTCCTTCTCCCTTTTTATATCCCATTT
1441 TTATATCGATCTCTTATTTTACAATAAAACTTTGCTGCCACCTGTGTGTCTGAGGGGTG
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' cuuuGGGUCGUCUGUUACAUCGa 5'
              |:||||   |:: ||||| 
Target 5' ctgcCTCAGCCTCCGGAGTAGCt 3'
 		1164 - 1186 115.00 -16.40
2 
miRNA  3' cuuuggGUCGUCUGUUACAUCga 5'
                |||| |:: | ||||  
Target 5' gttgggCAGCTGGTTAGGTAGag 3'
 		849 - 871 109.00 -11.50
3 
miRNA  3' cuuugggucgucUGUUACAUCGa 5'
                      |||:||| || 
Target 5' agatggggtctcACAGTGTTGCc 3'
 		1239 - 1261 107.00 -10.20
Experimental Support 1 for Non-Functional miRNA-Target Interaction
miRNA:Target hsa-miR-221 :: TP53    [ Non-Functional MTI ]
Validation Method Western blot
Conditions 293T, OECM-1
Original Description (Extracted from the article) ... Western blot analysis indicated that there was remark- able downregulation of p27 and p57 in OECM-1-miR-221/miR-222 and SAS-miR-222 cells. However, only a slight downregulation of p27 and p57 in SAS-miR-221 cells was noted. Furthermore, p21 was downregulated in SAS-miR-221 and SAS-miR-222 cells relative to the controls. However, ectopic miR-221/miR-222 expression did not change p53 expression (Fig. 3). ...

- Yang, C. J. Shen, W. G. Liu, C. J. Chen, Y. et al., 2011, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology.
Article - Yang, C. J. Shen, W. G. Liu, C. J. Chen, Y. et al.
- Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 2011
J Oral Pathol Med (2011) Backgrounds: MicroRNAs are small noncoding RNAs involved in posttranscriptional gene regulation, which play an important role in both physiological functioning and pathological progression. The miR-221/miR-222 microRNA family has been shown to be related to the neoplastic process in a number of different types of cancers; nevertheless, its function in oral squamous cell carcinoma (OSCC) remained uncertain. Materials and Methods: Paired OSCC and matched noncancerous oral mucosa were examined for miR-221/miR-222 expression using quantitative reverse-transcription PCR. Ectopic expression of miR-221/miR-222 by lentiviral infection was investigated to explore its in vitro and in vivo impact on the oncogenic phenotype and the expression of various target genes. The expression of Cip/Kip cell cycle regulator p27 in tumors was analyzed with immunohistochemistry. Results: The expression levels of miR-221 and miR-222 were highly correlated in OSCC. Increased miR-221/miR-222 expression was found in 40% of OSCC tissues. The ectopic expression of miR-221 or of miR-222 increased growth and anchorage-independent colony formation of OSCC cell lines. It also resulted in an increase in the tumorigenesis of an OSCC cell line in nude mice. Western blot analysis suggested that p27 and p57 might be the targets of miR-221/miR-222. p27 expression was reversely associated with the miR-221 and miR-222 expression level in OSCC tissues. Conclusions: Our findings suggested that increased miR-221/miR-222 expression was associated with the OSCC cell growth.
LinkOut: [PMID: 21226887]