Accession ID: MIRT005823 [miRNA, hsa-miR-204 :: CXCL3, target gene]
|Description||Homo sapiens miR-204 stem-loop|
|Comment||This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences .|
|2nd Structure of pre-miRNA|
|Mature Sequence||72| GCUGGGAAGGCAAAGGGACGU |92|
|Putative hsa-miR-204-3p Targets|
|Mature Sequence||33| UUCCCUUUGUCAUCCUAUGCCU |54|
|Putative hsa-miR-204-5p Targets|
|Synonyms||CINC-2b, GRO3, GROg, MIP-2b, MIP2B, SCYB3|
|Description||chemokine (C-X-C motif) ligand 3|
hsa04060 Cytokine-cytokine receptor interaction - Homo sapiens (human)
hsa04062 Chemokine signaling pathway - Homo sapiens (human)
|Putative miRNA Targets on CXCL3|
|3'UTR of CXCL3
(miRNA target sites are highlighted)
>CXCL3|NM_002090|3'UTR 1 TGACAGGAGAGAAGTAAGAAGCTTATCAGCGTATCATTGACACTTCCTGCAGGGTGGTCCCTGCCCTTACCAGAGCTGAA 81 AATGAAAAAGAGAACAGCAGCTTTCTAGGGACAGCTGGAAAGGACTTAATGTGTTTGACTATTTCTTACGAGGGTTCTAC 161 TTATTTATGTATTTATTTTTGAAAGCTTGTATTTTAATATTTTACATGCTGTTATTTAAAGATGTGAGTGTGTTTCATCA 241 AACATAGCTCAGTCCTGATTATTTAATTGGAATATGATGGGTTTTAAATGTGTCATTAAACTAATATTTAGTGGGAGACC 321 ATAATGTGTCAGCCACCTTGATAAATGACAGGGTGGGGAACTGGAGGGTGGGGGGATTGAAATGCAAGCAATTAGTGGAT 401 CACTGTTAGGGTAAGGGAATGTATGTACACATCTATTTTTTATACTTTTTTTTTAAAAAAAGAATGTCAGTTGTTATTTA 481 TTCAAATTATCTCACATTATGTGTTCAACATTTTTATGCTGAAGTTTCCCTTAGACATTTTATGTCTTGCTTGTAGGGCA 561 TAATGCCTTGTTTAATGTCCATTCTGCAGCGTTTCTCTTTCCCTTGGAAAAGAGAATTTATCATTACTGTTACATTTGTA 641 CAAATGACATGATAATAAAAGTTTTATGAAAAAAAAAAAAAAA
|miRNA-target interactions (Predicted by miRanda)||
|miRNA:Target||hsa-miR-204 :: CXCL3 [ Functional MTI ]|
|Original Description (Extracted from the article)||... A total of 48 probe sets corresponding to 34 genes showed a significant down-regulation higher than 1.5 fold (table 3). As shown in table 3, a total of 23 down-regulated genes were predicted targets of miR-204 in at least one of the following databases: Microcosm, TargetScan and PicTar-Vert. Only one of these genes, TGFβR2 has been previously experimentally validated as a miR-204 target. ...
- Li, G. Luna, C. Qiu, J. Epstein, D. L. et al., 2011, Investigative Ophthalmology & Visual Science.
Role of miR-204 in the regulation of apoptosis, ER stress response, and inflammation in human trabecular meshwork cells- Li, G. Luna, C. Qiu, J. Epstein, D. L. et al.
- Investigative Ophthalmology & Visual Science, 2011
Purpose. To investigate the biological functions of miR-204 in human trabecular meshwork (HTM) cells. Methods. Changes in gene expression induced by miR-204 in HTM cells were evaluated by gene array analysis using Affymetrix U133A2 arrays and confirmed by quantitative-PCR (Q-PCR). Direct targeting of miR-204 to twelve potential novel targets was confirmed using the psiCheck2 luciferase system, and five of them were verified by Western blot. Effects of miR-204 on apoptosis, cell viability, and accumulation of carbonylated proteins were evaluated in HTM cells treated with H(2)O(2). Induction of endoplasmic reticulum (ER) stress markers by tunicamycin was analyzed by Q-PCR and expression of IL-8 and IL-11 by ELISA. Results. MiR-204 decreased the expression of multiple genes in HTM cells. Twelve genes (AP1S2, Bcl2l2, BIRC2, EDEM1, EZR, FZD1, M6PR, RAB22A, RAB40B, SERP1, TCF12 and TCF4) were validated as direct targets of miR-204. Down-regulation of expressions at protein levels of Bcl2l2, BIRC2, EZR, M6PR and SERP1 were confirmed by Western blot. HTM cells transfected with miR-204 showed increased levels of apoptosis, decreased viability, and increased accumulation of oxidized proteins after H(2)O(2) treatment, as well as decreased induction of ER stress response markers and reduced expression of inflammatory mediators IL-8 and IL-11. Conclusions. MiR-204 potentially plays an important role in the regulation of multiple functions in HTM cells including apoptosis, accumulation of damaged proteins, ER stress response, and expression of inflammatory mediators.LinkOut: [PMID: 21282569]