Accession ID: MIRT005823 [miRNA, hsa-miR-204 :: CXCL3, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-204 LinkOut: [miRBase ]
Description Homo sapiens miR-204 stem-loop
Comment This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences .
2nd Structure of pre-miRNA
Mature miRNA Information
Mature miRNA hsa-miR-204-3p
Mature Sequence 72| GCUGGGAAGGCAAAGGGACGU |92
Evidence Not_experimental
Experiments
Putative hsa-miR-204-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Mature miRNA hsa-miR-204-5p
Mature Sequence 33| UUCCCUUUGUCAUCCUAUGCCU |54
Evidence Experimental
Experiments Cloned
Putative hsa-miR-204-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
miRNA-target interaction network
Gene Information
Gene Symbol CXCL3 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms CINC-2b, GRO3, GROg, MIP-2b, MIP2B, SCYB3
Description chemokine (C-X-C motif) ligand 3
Transcript NM_002090   LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
KEGG Pathway hsa04060    Cytokine-cytokine receptor interaction - Homo sapiens (human)
hsa04062    Chemokine signaling pathway - Homo sapiens (human)
Putative miRNA Targets on CXCL3 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of CXCL3
(miRNA target sites are highlighted)		 
>CXCL3|NM_002090|3'UTR
   1 TGACAGGAGAGAAGTAAGAAGCTTATCAGCGTATCATTGACACTTCCTGCAGGGTGGTCCCTGCCCTTACCAGAGCTGAA
  81 AATGAAAAAGAGAACAGCAGCTTTCTAGGGACAGCTGGAAAGGACTTAATGTGTTTGACTATTTCTTACGAGGGTTCTAC
 161 TTATTTATGTATTTATTTTTGAAAGCTTGTATTTTAATATTTTACATGCTGTTATTTAAAGATGTGAGTGTGTTTCATCA
 241 AACATAGCTCAGTCCTGATTATTTAATTGGAATATGATGGGTTTTAAATGTGTCATTAAACTAATATTTAGTGGGAGACC
 321 ATAATGTGTCAGCCACCTTGATAAATGACAGGGTGGGGAACTGGAGGGTGGGGGGATTGAAATGCAAGCAATTAGTGGAT
 401 CACTGTTAGGGTAAGGGAATGTATGTACACATCTATTTTTTATACTTTTTTTTTAAAAAAAGAATGTCAGTTGTTATTTA
 481 TTCAAATTATCTCACATTATGTGTTCAACATTTTTATGCTGAAGTTTCCCTTAGACATTTTATGTCTTGCTTGTAGGGCA
 561 TAATGCCTTGTTTAATGTCCATTCTGCAGCGTTTCTCTTTCCCTTGGAAAAGAGAATTTATCATTACTGTTACATTTGTA
 641 CAAATGACATGATAATAAAAGTTTTATGAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1 
miRNA  3' ugCAGGGAAAC-GGAAGGGUCg 5'
            |||||  || |||| |||| 
Target 5' tgGTCCC--TGCCCTTACCAGa 3'
 		55 - 74 135.00 -22.00
2 
miRNA  3' ugCAGGGAAACGGAAGGGucg 5'
            ||::||   |:|||||   
Target 5' gcGTTTCT---CTTTCCCttg 3'
 		589 - 606 116.00 -16.30
3 
miRNA  3' ugcagggaaacgGAAGGGUCg 5'
                      |||:|:|| 
Target 5' agagaacagcagCTTTCTAGg 3'
 		89 - 109 113.00 -8.20
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-204 :: CXCL3    [ Functional MTI ]
Validation Method Microarray
Conditions HTM1073
Original Description (Extracted from the article) ... A total of 48 probe sets corresponding to 34 genes showed a significant down-regulation higher than 1.5 fold (table 3). As shown in table 3, a total of 23 down-regulated genes were predicted targets of miR-204 in at least one of the following databases: Microcosm, TargetScan and PicTar-Vert. Only one of these genes, TGFβR2 has been previously experimentally validated as a miR-204 target. ...

- Li, G. Luna, C. Qiu, J. Epstein, D. L. et al., 2011, Investigative Ophthalmology & Visual Science.
Article - Li, G. Luna, C. Qiu, J. Epstein, D. L. et al.
- Investigative Ophthalmology & Visual Science, 2011
Purpose. To investigate the biological functions of miR-204 in human trabecular meshwork (HTM) cells. Methods. Changes in gene expression induced by miR-204 in HTM cells were evaluated by gene array analysis using Affymetrix U133A2 arrays and confirmed by quantitative-PCR (Q-PCR). Direct targeting of miR-204 to twelve potential novel targets was confirmed using the psiCheck2 luciferase system, and five of them were verified by Western blot. Effects of miR-204 on apoptosis, cell viability, and accumulation of carbonylated proteins were evaluated in HTM cells treated with H(2)O(2). Induction of endoplasmic reticulum (ER) stress markers by tunicamycin was analyzed by Q-PCR and expression of IL-8 and IL-11 by ELISA. Results. MiR-204 decreased the expression of multiple genes in HTM cells. Twelve genes (AP1S2, Bcl2l2, BIRC2, EDEM1, EZR, FZD1, M6PR, RAB22A, RAB40B, SERP1, TCF12 and TCF4) were validated as direct targets of miR-204. Down-regulation of expressions at protein levels of Bcl2l2, BIRC2, EZR, M6PR and SERP1 were confirmed by Western blot. HTM cells transfected with miR-204 showed increased levels of apoptosis, decreased viability, and increased accumulation of oxidized proteins after H(2)O(2) treatment, as well as decreased induction of ER stress response markers and reduced expression of inflammatory mediators IL-8 and IL-11. Conclusions. MiR-204 potentially plays an important role in the regulation of multiple functions in HTM cells including apoptosis, accumulation of damaged proteins, ER stress response, and expression of inflammatory mediators.
LinkOut: [PMID: 21282569]