Accession ID: MIRT005846 [miRNA, hsa-miR-204 ::
TCF4, target gene]
| pre-miRNA ID | hsa-mir-204 LinkOut: [miRBase ] |
|---|---|
| Description | Homo sapiens miR-204 stem-loop |
| Comment | This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences . |
| 2nd Structure of pre-miRNA |  |
| Mature miRNA | hsa-miR-204-3p |
|---|---|
| Mature Sequence | 72| GCUGGGAAGGCAAAGGGACGU |92 |
| Evidence | Not_experimental |
| Experiments | |
| Putative hsa-miR-204-3p Targets | LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ] |
| Mature miRNA | hsa-miR-204-5p |
| Mature Sequence | 33| UUCCCUUUGUCAUCCUAUGCCU |54 |
| Evidence | Experimental |
| Experiments | Cloned |
| Putative hsa-miR-204-5p Targets | LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ] |
 |
| Gene Symbol | TCF4 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ] | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Synonyms | E2-2, ITF2, MGC149723, MGC149724, PTHS, SEF2, SEF2-1, SEF2-1A, SEF2-1B, bHLHb19 | ||||||||||||||||||||
| Description | transcription factor 4 | ||||||||||||||||||||
| Transcript | NM_001083962 LinkOut: [ RefSeq ] | ||||||||||||||||||||
| Other Transcripts | NM_003199 | ||||||||||||||||||||
| Expression | LinkOut: [ BioGPS ] | ||||||||||||||||||||
| Putative miRNA Targets on TCF4 | LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ] | ||||||||||||||||||||
| 3'UTR of TCF4 (miRNA target sites are highlighted) |
>TCF4|NM_001083962|3'UTR 1 TAAAAGGGTCCAAGTTGCCACATTGCTTCATTAAAACAAGAGACCACTTCCTTAACAGCTGTATTATCTTAAACCCACAT 81 AAACACTTCTCCTTAACCCCCATTTTTGTAATATAAGACAAGTCTGAGTAGTTATGAATCGCAGACGCAAGAGGTTTCAG 161 CATTCCCAATTATCAAAAAACAGAAAAACAAAAAAAAGAAAGAAAAAAGTGCAACTTGAGGGACGACTTTCTTTAACATA 241 TCATTCAGAATGTGCAAAGCAGTATGTACAGGCTGAGACACAGCCCAGAGACTGAACGGCAATCTTTCCACACTGTGGAA 321 CAATGCATTTGTGCCTAAACTTCTTTTGGAAAAAAAAAATATAATTAATTTGTAAGTCTGAAAAAAAAATATTTAATTTA 401 AAAAAAATTGTAAACTTGCAATAATGAAAAAGTGTACTTCTGAAGAAAACTACATGAACGTTTTTGTTGGTATTCAAGTC 481 AGCTAGTGTTTATAATTACTGGATATTGAATTAGGGGAAGCTCGGCTGCCCTAGTAACAAAACCAGCAAACGTCCTGATG 561 ACAACGAAGTGATGACATTAGCCATTCCTTAGGGTAGGAGGAACAGATGGATCTTATAGACCTATGACAAATATATATAT 641 AAATATATATATAAATATATATTAAAAATTTAGTGACTATGGTAAGCTTTTGTTCATTTGTTTCAGACTTTTTTCTCCTG 721 TAAAAAAATAGTACTGATTAACTTTTTTAAAAGAAAGATTTTACTGTAAATATGGATTTTTTTTTTTTTGGTCTTATTTC 801 TGTCCCTTTCCCTGGTTTGTTATCGTAACCTGTAGTGCCAACTCTGCTTCCAGAGGGGTAGTGCAGGATGAAATGCTGAC 881 CCTGATGTTGCTTCTCATTCATAAATAAGTAGAAAGTTGTTTCTCCAGTCTTTTGGGAACACAGGACTTAAAAGTCACAT 961 CATGTGTAGATATTACAAGCAGCATTACCAAGACATGGCAAAAAGAGTTTGTCTGAATTGTAATGTTGCGTTTGTGAACC 1041 TATTCTGGGATTTTCAGAGGTACAAGGTTAGAATGCTACAATGTTACCACTGTGCCTTCCAATGTTTATATCATCGGAAA 1121 CATAACATAATCAAAGTGGCTGTGATTTAACAAAATGATTAAAGTGTTACCTACCTGTGTAGCCGAAGTAGTGTGCAGTG 1201 AGGCGTTTCTGAATACATGGTCAGATTTTTGGAAAAAAACAAAAACAAAAAAAACAAGTAAAGTTCAAAAACCGTCAAAT 1281 GAGAAAATTGCAAGTAGTGTGACAGAGCTGATTGATTTTGTTGCTTTCTTGATTTTTTTTTTCAAAATGGGTTTACTAAA 1361 ATGTAGATGACTTAACTGCCTCCTCCTTCGTCTGAAAAATGCCAATATTCAATCATCATGCAGCATTATAACAAGCCTTA 1441 TAAGTCCTAAAGCATTAAGTTGCACTTTTTTGAGGAGGGGTAGTGCAGTATTTCTCTGGCCAGTATGAATGAAGTTTATA 1521 CTTACCATATTTGATAGAAACATAGATCAAGCTATGGCACAGCGACTCATCAGATAGCTAGCTTTGACGTCTGGGCACAA 1601 TTGAACCAACTTCCATCGTGAATCTTTATAATGATTGACTTTGGTGTATAGTGCAGTAAACAAATAGTGCTCCTAGTTAA 1681 GTATTTGTCAGCATCCTTTTGTCTCTAACTTGTTTCTATTTTTACAGCCACACAATTCTTGGCATGTATTAAGAAAAAAA 1761 AAAATCCCTGTTCAAGTAGTTTTTCCACCTATCAGCACTGAGTAAATGCCATAAATCCATTGAAATGGTCTAAATGTTCC 1841 ATCTGTTCTCCTGTTTTGCCAGTTATATAGTAATGAAATACATTTGTAAATTTTATGCAACAAATGGCAAACGTATCATT 1921 ATTTTGAAATTGTGTATGTAAAAGTTATATTTTTACATGTAGACTCTTGTTATTATGTGTTTTAATACATTGTATCAGTT 2001 TTTGTTTTTTTTTAAAAACTGTGGTTTAAAAAGAAGTCTCATTTAAATGAAATAGCTACAAGAATCAGAATTTTATGTTC 2081 ATTTCTGAAAATGTAAGAACAAATAAGATAGTTACCACGTGGTCATCTTTTACAAACCCATAAACATTTTGATTAGCTGT 2161 GTGTGTGTTGAAAAACTGTAAATATGTTCAGTAGCGATAAAACTAAAATAACTTTGATTTGTTGATAAGTTCCTAAAATG 2241 TGGAGGTGGATTAAAACCTTAGGAGAATAGCAGAAATCAAACTTCATGAAAAGTTATTTTGGGGCTTTCCTGTGAAATGT 2321 ATGAACAAAGAGGCTCAGAGAAGGACATGGAAGACAATAATGTATACTCTCTCCTCCTCCCTGAATAATGAAAACCATGT 2401 GTATTTGTTCCCTCCGTATGTTAAAGATTTCCTTTTAGTGGTACATTCTGCACTCATTTTGTATAGTCTACCAAGGCGGG 2481 TATCCCTAGGAACAATATTATATAGGAAGCAGGTATACTCTGATCACATTCAGGATAAGTGTACAGAAGAAAATACGGTG 2561 TTTACTCTTTAGGGAACTGGAAACACTCCCTGCATTGATGTACATTTTAAGAATGGCACTTTTGATACATGTTATCATAA 2641 AGGTGCTTAATAGAGCTGAATTAAAGTTTTTCAAATCTGTAAACAAAGCAAAAAAGTAAATTGTAGTCATTTGATTATTT 2721 TTTAAATTGGTGCTTTATATTTTGTTCTCACTCAGAGTAAAAGCTGCAATTTATTGTTCACCAGCTTTGATGTATTCATT 2801 ACTCAGTAATGCAATACCTCTATTGTTGAATTCCCTTTGGAAATAAGTGAAAATTCTAACGGCCACTGAAAGCTGCTCGC 2881 TAGGTTTTGCTTGGTGGAGAAACATAATCTGCACCTATCCATATTAATTGGGTTGTATCCCCATTAAAAAAGAAAAAAAG 2961 GGAATGTGGCCTTTTTAGTGTGTTTTTTATTGTTGTTGTTTTGTAATTATCAAACCCAGGTAAGATATTGGTATCCTGCA 3041 CTGGATTTTCAAATGAAGTTCAGCAGAAGACAGTTAAGATTAAAGTACTATACAAAAATTTCAAAAGGGTCCATACTACG 3121 CTATCTGTATGACGACACTTAGGCTGGGGATCTCTTTCAGAAACTCGGACTTTAAAAGCAACTTGGAGCAGTTGATCCAC 3201 CTCCACATTCAAGTAATTTATGAATATGCAGAATAGGGATCTGTTCATCTAGAAATTTTTACCATTTGTCTTCTGTGTAG 3281 CTGCAAGGAACACTAATGTTTATACAACTGTCAGTCCACCCAGTGGTGCAACTGGTTCTGATTCAGTCTTCCGATTCCTT 3361 TTTATTTTTCACTTTTTCCTATTTCTGAATTTTTTTTTTTATTTGTGATCTTGATTTTGATGAGGGGTTGGGGAGTGGGG 3441 AGGGAGTCGAACCAAGACTTGGAGTTAAGAGGATTTTCATCTTTTGCATCCAACAGGCAGAATATGATCTGTGTCCAAAA 3521 GTGAACTTGAGTCAGGAATGAATCAATTTCAGCATAAACAAGCACAAAAATTTAGTCTGCTGGCTGACTGGAAGCAAAAA 3601 AGTCAAGATGGAATATGATGAATTCCAACACAATGGGGCACCAAGGCCTTTAGGCCTCTCTTTTTATTTTGCTTTGGTTT 3681 TGTTTGTTTTTCTTTAGAGACATGCTCTTTCTCATGGGACTTGAAGTGGACTCATCTTTGTGCAGTGCTGGTTTTGCCAT 3761 ACTCATTTCAAGTATTATAGACATATGTAATGGTGAAAATATATGAACTGTGGCCTTTTTCATTCTTGTTACTTGTGATG 3841 CAATTAAGTGAAGATAAGAAAAAAAAAAAAAAAGCAGAGATTTACCATGTATCAGTGCCTGGCTTTTTGTTATAAAGCTT 3921 TGTTTGTCTAGTGCTCTTTTGCTATAAAATAGACTGTAGTACACCCTAGTAGGAAAAAAAAAAAACTAAATTTAAAAATA 4001 AAAAATATATTTGGCTTATTTTTCGCAGGAGCAATCCTTTTATACCATGAATATTACAAAAAAATTGTCAGATTCTGAAT 4081 ATTTCTTCTTTGTAGATTTTTGGAATCATTATGAGTAAAAGTTTGTTACTTTATTTTACTATTTAAAAGATGTTATTTTA 4161 CCATGTGTTACCAAGATGAAACTGTATGGGTAGCTTTTTTGTTTGTTTTTTGTTTTGTTTTTGTTTTTGTTTTTGTTTTT 4241 AGTTGTAGGTCGCAGCGGGGAAATTTTTTGCGACTGTACACATAGCTGCAGCATTAAAAACTTAAAAAAATTGTTAAAAA 4321 AAAAAAAAGGGAAAACATTTCAAAAAAAAAAAAAAAGATAAACAGTTACACCTTGTTTTCAATGTGTGGCTGAGTGCCTC 4401 GATTTTTTCATGTTTTTGGTGTATTTCTGATTTGTAGAAGTGTCCAAACAGGTTGTGTGCTGGAGTTCCTTCAAGACAAA 4481 AACAAACCCAGCTTGGTCAAGGCCATTACCTGTTTCCCATCTGTAGTTATTCGATGAAGTCATGTACATGACCGTTCTGT 4561 AGCAATAAATGTGCCATTTTTATAAACTGTTTCTGACACTTGTTTCATTTCATTTTGCATTGTCCATATAGCTATGATTC 4641 TCTTCTGTAAGTAAAACGCATCTATATTTCATTTTCCAAGTGTTGGAGGTATTGACAGCTTAACAAACAAAACATACAAA 4721 AAAAATCACAAAAACAAATTGAAAAGCAAAGCACATGATTGATCAAGGAAGAGATGCCCTTAATGAAAATGGAACGGGAT 4801 GCATGCAAAACAAAAAGAAAACTGTCTAGAGGATTAACTAATTGAAGGAATATAATTAATGTGTGTGTAACACTGAAGCT 4881 ATGCATTTGAAGAGCTCTGAACTGCACCAGTGTTTTCGGTTGTGCTGCAGGTTGCTAAGTCAAGTCAGCCTTAACCTTTT 4961 GCACCAGTTGGTCGGCTGTTTGGCAGAACATTCTCAGATCTTTTCAGTCAAAAATCTAAGATGATTTATTTTGTATCACT 5041 TTGTTAAAAGCTGAATATTGTTAACTACAGTTAATATTAACACTGTATTTATACTTTCTCAAACTACATCCGCCCCACCA 5121 CTTCTGGTTGCCTCTGTTGACTATTAATCCAGATGTAAACAACCAGATGTTTTTTTCTAACTTGTACAAACTGACGTGTG 5201 TCAACTATCATGGAAGGAAAAAAATGTACAGATTAAAATTATTCAGTGTTATGTACTGTAAGTTAATATTTTTGTAGAAT 5281 GGACATCAATCTACTTTGCAAAATTTGGAGGCTATTTCAACATTGCACTGTAGAAATGTAAAGTAATGTATGCAATGTAA 5361 AGGAAAGCCCGCGGTAGCTGAGCGCTTCATAACAGAATGTTCTAATCAAGTACGTGGTATTTGGGGATGTCTCCAATATT 5441 GCTCTTGTATTCTTTCTAATTGGGTTTAGTGACTAGTTGAAGGAAAATGTTATAACGCCATTTGGTTCACATGTGAAGTG 5521 CCCTCCATAGCCAAATGTTGGGATTTTTTTTTTTTTCGTTTTTGGTTGGACTGTTTGCAGATATTTAAATTTTATGAAAT 5601 TTCCAAAGATTTTGGTTGATAACCCCCTTTTACCTTCTAAATGATTTGAGATGTTCTTATGTTCTTACTGTGTGTTTTAA 5681 ATATATATAAAAGAGCCACAAGCATTT Target sites Provided by authors Predicted by miRanda |
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| miRNA-target interactions (Predicted by miRanda) |
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| miRNA:Target | hsa-miR-204 :: TCF4 [ Functional MTI ] |
|---|---|
| Validation Method | Luciferase reporter assay , Microarray , qRT-PCR |
| Conditions | H293, HEK293, HTM1073 |
| Location of target site | 3'UTR |
| Tools used in this research | TargetScan |
| Original Description (Extracted from the article) | ... A total of 48 probe sets corresponding to 34 genes showed a significant down-regulation higher than 1.5 fold (table 3). As shown in table 3, a total of 23 down-regulated genes were predicted targets of miR-204 in at least one of the following databases: Microcosm, TargetScan and PicTar-Vert. Only one of these genes, TGFβR2 has been previously experimentally validated as a miR-204 target. ... - Li, G. Luna, C. Qiu, J. Epstein, D. L. et al., 2011, Investigative Ophthalmology & Visual Science. |
| Article |
- Li, G.
Luna, C.
Qiu, J.
Epstein, D. L. et al. - Investigative Ophthalmology & Visual Science, 2011
Purpose. To investigate the biological functions of miR-204 in human trabecular meshwork (HTM) cells. Methods. Changes in gene expression induced by miR-204 in HTM cells were evaluated by gene array analysis using Affymetrix U133A2 arrays and confirmed by quantitative-PCR (Q-PCR). Direct targeting of miR-204 to twelve potential novel targets was confirmed using the psiCheck2 luciferase system, and five of them were verified by Western blot. Effects of miR-204 on apoptosis, cell viability, and accumulation of carbonylated proteins were evaluated in HTM cells treated with H(2)O(2). Induction of endoplasmic reticulum (ER) stress markers by tunicamycin was analyzed by Q-PCR and expression of IL-8 and IL-11 by ELISA. Results. MiR-204 decreased the expression of multiple genes in HTM cells. Twelve genes (AP1S2, Bcl2l2, BIRC2, EDEM1, EZR, FZD1, M6PR, RAB22A, RAB40B, SERP1, TCF12 and TCF4) were validated as direct targets of miR-204. Down-regulation of expressions at protein levels of Bcl2l2, BIRC2, EZR, M6PR and SERP1 were confirmed by Western blot. HTM cells transfected with miR-204 showed increased levels of apoptosis, decreased viability, and increased accumulation of oxidized proteins after H(2)O(2) treatment, as well as decreased induction of ER stress response markers and reduced expression of inflammatory mediators IL-8 and IL-11. Conclusions. MiR-204 potentially plays an important role in the regulation of multiple functions in HTM cells including apoptosis, accumulation of damaged proteins, ER stress response, and expression of inflammatory mediators.
LinkOut: [PMID: 21282569]
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