Accession ID: MIRT005973 [miRNA, hsa-miR-99a-5p :: SERPINE1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-99aLinkOut: [miRBase ]
Description Homo sapiens miR-99a stem-loop
Comment This sequence is localised to chromosome 21 and was named mir-99-21 in reference .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-99a-5p
Mature Sequence 13| AACCCGUAGAUCCGAUCUUGUG |34
Evidence Experimental
Experiments Cloned
Putative hsa-miR-99a-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol SERPINE1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms PAI, PAI-1, PAI1, PLANH1
Description serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Transcript NM_0006    LinkOut: [ RefSeq ]
Other Transcripts NM_0011654   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on SERPINE1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of SERPINE1
(miRNA target sites are highlighted)
>SERPINE1|NM_0006|3'UTR
   1 TGACCCTGGGGAAAGACGCCTTCATCTGGGACAAAACTGGAGATGCATCGGGAAAGAAGAAACTCCGAAGAAAAGAATTT
  81 TAGTGTTAATGACTCTTTCTGAAGGAAGAGAAGACATTTGCCTTTTGTTAAAAGATGGTAAACCAGATCTGTCTCCAAGA
 161 CCTTGGCCTCTCCTTGGAGGACCTTTAGGTCAAACTCCCTAGTCTCCACCTGAGACCCTGGGAGAGAAGTTTGAAGCACA
 241 ACTCCCTTAAGGTCTCCAAACCAGACGGTGACGCCTGCGGGACCATCTGGGGCACCTGCTTCCACCCGTCTCTCTGCCCA
 321 CTCGGGTCTGCAGACCTGGTTCCCACTGAGGCCCTTTGCAGGATGGAACTACGGGGCTTACAGGAGCTTTTGTGTGCCTG
 401 GTAGAAACTATTTCTGTTCCAGTCACATTGCCATCACTCTTGTACTGCCTGCCACCGCGGAGGAGGCTGGTGACAGGCCA
 481 AAGGCCAGTGGAAGAAACACCCTTTCATCTCAGAGTCCACTGTGGCACTGGCCACCCCTCCCCAGTACAGGGGTGCTGCA
 561 GGTGGCAGAGTGAATGTCCCCCATCATGTGGCCCAACTCTCCTGGCCTGGCCATCTCCCTCCCCAGAAACAGTGTGCATG
 641 GGTTATTTTGGAGTGTAGGTGACTTGTTTACTCATTGAAGCAGATTTCTGCTTCCTTTTATTTTTATAGGAATAGAGGAA
 721 GAAATGTCAGATGCGTGCCCAGCTCTTCACCCCCCAATCTCTTGGTGGGGAGGGGTGTACCTAAATATTTATCATATCCT
 801 TGCCCTTGAGTGCTTGTTAGAGAGAAAGAGAACTACTAAGGAAAATAATATTATTTAAACTCGCTCCTAGTGTTTCTTTG
 881 TGGTCTGTGTCACCGTATCTCAGGAAGTCCAGCCACTTGACTGGCACACACCCCTCCGGACATCCAGCGTGACGGAGCCC
 961 ACACTGCCACCTTGTGGCCGCCTGAGACCCTCGCGCCCCCCGCGCCCCTCTTTTTCCCCTTGATGGAAATTGACCATACA
1041 ATTTCATCCTCCTTCAGGGGATCAAAAGGACGGAGTGGGGGGACAGAGACTCAGATGAGGACAGAGTGGTTTCCAATGTG
1121 TTCAATAGATTTAGGAGCAGAAATGCAAGGGGCTGCATGACCTACCAGGACAGAACTTTCCCCAATTACAGGGTGACTCA
1201 CAGCCGCATTGGTGACTCACTTCAATGTGTCATTTCCGGCTGCTGTGTGTGAGCAGTGGACACGTGAGGGGGGGGTGGGT
1281 GAGAGAGACAGGCAGCTCGGATTCAACTACCTTAGATAATATTTCTGAAAACCTACCAGCCAGAGGGTAGGGCACAAAGA
1361 TGGATGTAATGCACTTTGGGAGGCCAAGGCGGGAGGATTGCTTGAGCCCAGGAGTTCAAGACCAGCCTGGGCAACATACC
1441 AAGACCCCCGTCTCTTTAAAAATATATATATTTTAAATATACTTAAATATATATTTCTAATATCTTTAAATATATATATA
1521 TATTTTAAAGACCAATTTATGGGAGAATTGCACACAGATGTGAAATGAATGTAATCTAATAGAAGCCTAATCAGCCCACC
1601 ATGTTCTCCACTGAAAAATCCTCTTTCTTTGGGGTTTTTCTTTCTTTCTTTTTTGATTTTGCACTGGACGGTGACGTCAG
1681 CCATGTACAGGATCCACAGGGGTGGTGTCAAATGCTATTGAAATTGTGTTGAATTGTATGCTTTTTCACTTTTGATAAAT
1761 AAACATGTAAAAATGTTTCAAAAAAATAATAAAATAAATAAATACGAAGAATATGTCAGGACAGTCAAAAAAAAAAAAAA
1841 AAAA
Target sites Provided by authors  Predicted by miRanda
Experimental Support 1 for Non-Functional miRNA-Target Interaction
miRNA:Target hsa-miR-99a-5p :: SERPINE1    [ Non-Functional MTI ]
Validation Method Luciferase reporter assay
Conditions HPMVEC
Location of target site 3'UTR
Tools used in this research MicroCosm
Original Description (Extracted from the article) ... Our results show reduced expression of miR-30c and miR-301a, but not of miR-99a in response to PlGF, which have evolutionarily conserved binding sites in the 3'-untranslated region (3'UTR) of PAI-1 mRNA. Luciferase reporter assays using wild-type and mutant 3’UTR constructs confirmed that PAI-1-3’UTR is indeed a direct target of miR-30c and miR-301a. ...

- Patel, N. Tahara, S. M. Malik, P. Kalra, V. K., 2011, The Biochemical journal.

Article - Patel, N. Tahara, S. M. Malik, P. Kalra, V. K.
- The Biochemical journal, 2011
PAI-1 (plasminogen activator inhibitor-1) is a key physiological inhibitor of fibrinolysis. Previously, we have reported PlGF (placental growth factor)-mediated transcriptional up-regulation of PAI-1 (SERPINE1) mRNA expression via activation of HIF-1alpha (hypoxia-inducible factor-1alpha) and AP-1 (activator protein-1) in HPMVECs (human pulmonary microvascular endothelial cells), which resulted in elevated PAI-1 in humans with SCA (sickle cell anaemia). In the present study, we have identified the role of post-transcriptional mechanism(s) of PlGF-mediated accumulation of PAI-1 mRNA in HPMVECs by examining the role of microRNAs (miRNAs/miRs) in PlGF-induced PAI-1 mRNA stability. Our results show reduced expression of miR-30c and miR-301a, but not of miR-99a, in response to PlGF, which have evolutionarily conserved binding sites in the 3'-UTR (3'-untranslated region) of PAI-1 mRNA. Transfection of anti-miR-30c or anti-miR-301a oligonucleotides resulted in increased PAI-1 mRNA levels, which were increased further with PlGF stimulation. Conversely, overexpression of pre-miR-30c or pre-miR-301a resulted in an attenuation of PlGF-induced PAI-1 mRNA and protein levels. Luciferase reporter assays using wild-type and mutant 3'-UTR constructs confirmed that the PAI-1 3'-UTR is indeed a direct target of miR-30c and miR-301a. Finally, plasma levels of miR-30c and miR-301a were significantly down-regulated in patients with SCA compared with normal controls. These results provide a post-transcriptional regulatory mechanism of PlGF-induced PAI-1 elevation.
LinkOut: [PMID: 21175428]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
120 hsa-miR-99a-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT002306 RAVER2 ribonucleoprotein, PTB-binding 2 3 1
MIRT004243 FGFR3 fibroblast growth factor receptor 3 4 2
MIRT005973 SERPINE1 serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 1 1
MIRT006145 IGF1R insulin-like growth factor 1 receptor 4 3
MIRT006146 MTOR mechanistic target of rapamycin (serine/threonine kinase) 4 5
MIRT027393 RPTOR regulatory associated protein of MTOR, complex 1 1 1
MIRT027394 AGO2 eukaryotic translation initiation factor 2C, 2 1 1
MIRT027395 MEF2D myocyte enhancer factor 2D 1 1
MIRT027396 SKI v-ski sarcoma viral oncogene homolog (avian) 1 1
MIRT027397 COQ2 coenzyme Q2 homolog, prenyltransferase (yeast) 1 1
MIRT027398 TRIB1 tribbles homolog 1 (Drosophila) 1 1
MIRT048601 MRPS33 mitochondrial ribosomal protein S33 1 1
MIRT048602 CCDC6 coiled-coil domain containing 6 1 1
MIRT048603 RASAL2 RAS protein activator like 2 1 1
MIRT048604 LRIG3 leucine-rich repeats and immunoglobulin-like domains 3 1 1
MIRT048605 DDX18 DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 1 1
MIRT048606 ND3 NADH dehydrogenase, subunit 3 (complex I) 1 1
MIRT048607 METTL23 chromosome 17 open reading frame 95 1 1
MIRT048608 MAPK6 mitogen-activated protein kinase 6 1 1
MIRT048609 ZNF367 zinc finger protein 367 1 1
MIRT048610 APEX1 APEX nuclease (multifunctional DNA repair enzyme) 1 1 1
MIRT048611 N4BP2 NEDD4 binding protein 2 1 1
MIRT048612 RCN2 reticulocalbin 2, EF-hand calcium binding domain 1 1
MIRT048613 RPL36A ribosomal protein L36a 1 1
MIRT048614 GXYLT1 glucoside xylosyltransferase 1 1 1
MIRT048615 DDX3X DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked 1 1
MIRT048616 EIF5A eukaryotic translation initiation factor 5A 1 1
MIRT048617 CIRH1A cirrhosis, autosomal recessive 1A (cirhin) 1 1
MIRT048618 STEAP4 STEAP family member 4 1 1
MIRT048619 RPS26 ribosomal protein S26 1 1
MIRT048620 DLD dihydrolipoamide dehydrogenase 1 1
MIRT048621 ORMDL1 ORM1-like 1 (S. cerevisiae) 1 1
MIRT048622 RARS arginyl-tRNA synthetase 1 1
MIRT048623 CUL2 cullin 2 1 1
MIRT048624 FOSL2 FOS-like antigen 2 1 1
MIRT048625 GPR126 G protein-coupled receptor 126 1 1
MIRT048626 GNAL guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type 1 1
MIRT048627 MON1B MON1 homolog B (yeast) 1 1
MIRT048628 SPIRE1 spire homolog 1 (Drosophila) 1 1
MIRT048629 TOMM22 translocase of outer mitochondrial membrane 22 homolog (yeast) 1 1
MIRT048630 ORC5 origin recognition complex, subunit 5-like (yeast) 1 1
MIRT048631 NDE1 nudE nuclear distribution gene E homolog 1 (A. nidulans) 1 1
MIRT048632 TNRC6B trinucleotide repeat containing 6B 1 1
MIRT048633 UBB ubiquitin B 1 1
MIRT048634 SRPK1 SFRS protein kinase 1 1 1
MIRT048635 CAPNS1 calpain, small subunit 1 1 1
MIRT048636 RPS6 ribosomal protein S6 1 1
MIRT048637 SNRNP200 small nuclear ribonucleoprotein 200kDa (U5) 1 1
MIRT048638 SRSF10 splicing factor, arginine/serine-rich 13A 1 1
MIRT048639 HADHB hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit 1 1
MIRT048640 LMAN2 lectin, mannose-binding 2 1 1
MIRT048641 GTF2H1 general transcription factor IIH, polypeptide 1, 62kDa 1 1
MIRT048642 N6AMT1 N-6 adenine-specific DNA methyltransferase 1 (putative) 1 1
MIRT048643 SGPP2 sphingosine-1-phosphate phosphatase 2 1 1
MIRT048644 GDE1 glycerophosphodiester phosphodiesterase 1 1 1
MIRT048645 VPS45 vacuolar protein sorting 45 homolog (S. cerevisiae) 1 1
MIRT048646 FAM171B family with sequence similarity 171, member B 1 1
MIRT048647 UQCR10 ubiquinol-cytochrome c reductase, complex III subunit X 1 1
MIRT048648 PUM1 pumilio homolog 1 (Drosophila) 1 1
MIRT048649 AKAP2 A kinase (PRKA) anchor protein 2 1 1
MIRT048650 EXTL3 exostoses (multiple)-like 3 1 1
MIRT048651 NFU1 NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae) 1 1
MIRT048652 UBA2 ubiquitin-like modifier activating enzyme 2 1 1
MIRT048653 RB1 retinoblastoma 1 1 1
MIRT048654 RPL7L1 ribosomal protein L7-like 1 1 1
MIRT048655 PLSCR1 phospholipid scramblase 1 1 1
MIRT048656 WHAMM WAS protein homolog associated with actin, golgi membranes and microtubules 1 1
MIRT048657 ITM2B integral membrane protein 2B 1 1
MIRT048658 POGK pogo transposable element with KRAB domain 1 1
MIRT048659 SEC13 SEC13 homolog (S. cerevisiae) 1 1
MIRT048660 TPPP3 tubulin polymerization-promoting protein family member 3 1 1
MIRT048661 RPS15 ribosomal protein S15 1 1
MIRT048662 GTF2H2C general transcription factor IIH, polypeptide 2C 1 1
MIRT048663 TMEM209 transmembrane protein 209 1 1
MIRT048664 SYNJ2BP synaptojanin 2 binding protein 1 1
MIRT048665 MED12 mediator complex subunit 12 1 1
MIRT048666 DOCK11 dedicator of cytokinesis 11 1 1
MIRT048667 ATP5E ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit 1 1
MIRT048668 DHCR24 24-dehydrocholesterol reductase 1 1
MIRT048669 PPM1A protein phosphatase, Mg2+/Mn2+ dependent, 1A 1 1
MIRT048670 NPM3 nucleophosmin/nucleoplasmin 3 1 1
MIRT048671 MYL2 myosin, light chain 2, regulatory, cardiac, slow 1 1
MIRT048672 CBWD1 COBW domain containing 1 1 1
MIRT048673 JPH1 junctophilin 1 1 1
MIRT048674 GTF2I general transcription factor IIi 1 1
MIRT048675 NDUFA2 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa 1 1
MIRT048676 COL4A1 collagen, type IV, alpha 1 1 1
MIRT048677 FOXRED2 FAD-dependent oxidoreductase domain containing 2 1 1
MIRT048678 AGO1 eukaryotic translation initiation factor 2C, 1 1 1
MIRT048679 RUNDC3B RUN domain containing 3B 1 1
MIRT048680 COX2 cytochrome c oxidase subunit II 1 1
MIRT048681 TUBG1 tubulin, gamma 1 1 1
MIRT048682 DDX42 DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 1 1
MIRT048683 ADO 2-aminoethanethiol (cysteamine) dioxygenase 1 1
MIRT048684 ADCY9 adenylate cyclase 9 1 1
MIRT048685 CTC1 chromosome 17 open reading frame 68 1 1
MIRT048686 MCM8 minichromosome maintenance complex component 8 1 1
MIRT048687 ATP1A1 ATPase, Na+/K+ transporting, alpha 1 polypeptide 1 1
MIRT048688 DNAJA3 DnaJ (Hsp40) homolog, subfamily A, member 3 1 1
MIRT048689 RAD51C RAD51 homolog C (S. cerevisiae) 1 1
MIRT048690 DDX46 DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 1 1
MIRT048691 MARK2 MAP/microtubule affinity-regulating kinase 2 1 1
MIRT048692 RPL37A ribosomal protein L37a 1 1
MIRT048693 TYMS thymidylate synthetase 1 1
MIRT048694 DDHD1 DDHD domain containing 1 1 1
MIRT048695 SALL2 sal-like 2 (Drosophila) 1 1
MIRT048696 PSMA2 proteasome (prosome, macropain) subunit, alpha type, 2 1 1
MIRT048697 SUDS3 suppressor of defective silencing 3 homolog (S. cerevisiae) 1 1
MIRT048698 P2RY11 purinergic receptor P2Y, G-protein coupled, 11 1 1
MIRT048699 E4F1 E4F transcription factor 1 1 1
MIRT048700 NFE2L1 nuclear factor (erythroid-derived 2)-like 1 1 1
MIRT048701 GLUD1 glutamate dehydrogenase 1 1 1
MIRT048702 RNF187 ring finger protein 187 1 1
MIRT048703 CUL3 cullin 3 1 1
MIRT048704 AP2B1 adaptor-related protein complex 2, beta 1 subunit 1 1
MIRT048705 GOLGA3 golgin A3 1 1
MIRT048706 ZBTB4 zinc finger and BTB domain containing 4 1 1
MIRT048707 BRD7 bromodomain containing 7 1 1
MIRT048708 CERS1 LAG1 homolog, ceramide synthase 1 1 1
MIRT048709 TTC38 tetratricopeptide repeat domain 38 1 1