Accession ID: MIRT006329 [miRNA, hsa-miR-137 :: PTGS2, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-137LinkOut: [miRBase ]
Description Homo sapiens miR-137 stem-loop
Comment This miRNA sequence is predicted based on homology to a verified miRNA from mouse .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-137
Mature Sequence 59| UUAUUGCUUAAGAAUACGCGUAG |81
Evidence Experimental
Experiments Cloned
Putative hsa-miR-137 Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol PTGS2 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2
Description prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Transcript NM_0009    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on PTGS2 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of PTGS2
(miRNA target sites are highlighted)
>PTGS2|NM_0009|3'UTR
   1 AAGTCTAATGATCATATTTATTTATTTATATGAACCATGTCTATTAATTTAATTATTTAATAATATTTATATTAAACTCC
  81 TTATGTTACTTAACATCTTCTGTAACAGAAGTCAGTACTCCTGTTGCGGAGAAAGGAGTCATACTTGTGAAGACTTTTAT
 161 GTCACTACTCTAAAGATTTTGCTGTTGCTGTTAAGTTTGGAAAACAGTTTTTATTCTGTTTTATAAACCAGAGAGAAATG
 241 AGTTTTGACGTCTTTTTACTTGAATTTCAACTTATATTATAAGAACGAAAGTAAAGATGTTTGAATACTTAAACACTGTC
 321 ACAAGATGGCAAAATGCTGAAAGTTTTTACACTGTCGATGTTTCCAATGCATCTTCCATGATGCATTAGAAGTAACTAAT
 401 GTTTGAAATTTTAAAGTACTTTTGGTTATTTTTCTGTCATCAAACAAAAACAGGTATCAGTGCATTATTAAATGAATATT
 481 TAAATTAGACATTACCAGTAATTTCATGTCTACTTTTTAAAATCAGCAATGAAACAATAATTTGAAATTTCTAAATTCAT
 561 AGGGTAGAATCACCTGTAAAAGCTTGTTTGATTTCTTAAAGTTATTAAACTTGTACATATACCAAAAAGAAGCTGTCTTG
 641 GATTTAAATCTGTAAAATCAGTAGAAATTTTACTACAATTGCTTGTTAAAATATTTTATAAGTGATGTTCCTTTTTCACC
 721 AAGAGTATAAACCTTTTTAGTGTGACTGTTAAAACTTCCTTTTAAATCAAAATGCCAAATTTATTAAGGTGGTGGAGCCA
 801 CTGCAGTGTTATCTTAAAATAAGAATATTTTGTTGAGATATTCCAGAATTTGTTTATATGGCTGGTAACATGTAAAATCT
 881 ATATCAGCAAAAGGGTCTACCTTTAAAATAAGCAATAACAAAGAAGAAAACCAAATTATTGTTCAAATTTAGGTTTAAAC
 961 TTTTGAAGCAAACTTTTTTTTATCCTTGTGCACTGCAGGCCTGGTACTCAGATTTTGCTATGAGGTTAATGAAGTACCAA
1041 GCTGTGCTTGAATAATGATATGTTTTCTCAGATTTTCTGTTGTACAGTTTAATTTAGCAGTCCATATCACATTGCAAAAG
1121 TAGCAATGACCTCATAAAATACCTCTTCAAAATGCTTAAATTCATTTCACACATTAATTTTATCTCAGTCTTGAAGCCAA
1201 TTCAGTAGGTGCATTGGAATCAAGCCTGGCTACCTGCATGCTGTTCCTTTTCTTTTCTTCTTTTAGCCATTTTGCTAAGA
1281 GACACAGTCTTCTCATCACTTCGTTTCTCCTATTTTGTTTTACTAGTTTTAAGATCAGAGTTCACTTTCTTTGGACTCTG
1361 CCTATATTTTCTTACCTGAACTTTTGCAAGTTTTCAGGTAAACCTCAGCTCAGGACTGCTATTTAGCTCCTCTTAAGAAG
1441 ATTAAAAGAGAAAAAAAAAGGCCCTTTTAAAAATAGTATACACTTATTTTAAGTGAAAAGCAGAGAATTTTATTTATAGC
1521 TAATTTTAGCTATCTGTAACCAAGATGGATGCAAAGAGGCTAGTGCCTCAGAGAGAACTGTACGGGGTTTGTGACTGGAA
1601 AAAGTTACGTTCCCATTCTAATTAATGCCCTTTCTTATTTAAAAACAAAACCAAATGATATCTAAGTAGTTCTCAGCAAT
1681 AATAATAATGACGATAATACTTCTTTTCCACATCTCATTGTCACTGACATTTAATGGTACTGTATATTACTTAATTTATT
1761 GAAGATTATTATTTATGTCTTATTAGGACACTATGGTTATAAACTGTGTTTAAGCCTACAATCATTGATTTTTTTTTGTT
1841 ATGTCACAATCAGTATATCTTCTTTGGGGTTACCTCTCTGAATATTATGTAAACAATCCAAAGAAATGATTGTATTAAGA
1921 TTTGTGAATAAATTTTTAGAAATCTGATTGGCATATTGAGATATTTAAGGTTGAATGTTTGTCCTTAGGATAGGCCTATG
2001 TGCTAGCCCACAAAGAATATTGTCTCATTAGCCTGAATGTGCCATAAGACTGACCTTTTAAAATGTTTTGAGGGATCTGT
2081 GGATGCTTCGTTAATTTGTTCAGCCACAATTTATTGAGAAAATATTCTGTGTCAAGCACTGTGGGTTTTAATATTTTTAA
2161 ATCAAACGCTGATTACAGATAATAGTATTTATATAAATAATTGAAAAAAATTTTCTTTTGGGAAGAGGGAGAAAATGAAA
2241 TAAATATCATTAAAGATAACTCAGGAGAATCTTCTTTACAATTTTACGTTTAGAATGTTTAAGGTTAAGAAAGAAATAGT
2321 CAATATGCTTGTATAAAACACTGTTCACTGTTTTTTTTAAAAAAAAAACTTGATTTGTTATTAACATTGATCTGCTGACA
2401 AAACCTGGGAATTTGGGTTGTGTATGCGAATGTTTCAGTGCCTCAGACAAATGTGTATTTAACTTATGTAAAAGATAAGT
2481 CTGGAAATAAATGTCTGTTTATTTTTGTACTATTTAAAAATTGACAGATCTTTTCTGAAGA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' gaugcgCAUAAGAAUUCGUUAUu 5'
                ||| |: | ||||||| 
Target 5' atctaaGTAGTTCTCAGCAATAa 3'
1660 - 1682 157.00 -11.90
2
miRNA  3' gaUGCGCAUA---AGA----AUUCGUUAUu 5'
            | | || |   |:|    ||||||||| 
Target 5' aaAAGGGTCTACCTTTAAAATAAGCAATAa 3'
889 - 918 151.00 -12.40
3
miRNA  3' gaUGCGCAU-AAGAAU-----UCGUUAUu 5'
            |:|: || ||:|||     ||||||: 
Target 5' tcATGTCTACTTTTTAAAATCAGCAATGa 3'
504 - 532 145.00 -8.75
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-137 :: PTGS2    [ Functional MTI ]
Validation Method Luciferase reporter assay , Western blot
Conditions U87 , LN229
Location of target site 3'UTR
Tools used in this research miRanda
Original Description (Extracted from the article) ... miR-137 is able to inhibit Cox-2 protein expression through directly binding to 3' UTR region of Cox-2 mRNA. ...

- Chen, L. Wang, X. Wang, H. Li, Y. Yan, W. et al., 2012, Eur J Cancer.

Article - Chen, L. Wang, X. Wang, H. Li, Y. Yan, W. et al.
- Eur J Cancer, 2012
MicroRNAs are strongly implicated in cancer but their specific roles and functions in the major cancers have yet to be fully elucidated. In this study, we defined the expression and function of miR-137, which we found to be downregulated in glioma samples and glioma cells by qRT-PCR. Ectopic expression of miR-137 in glioma cell lines inhibited proliferation and invasion. Using computational and expression analysis, Cox-2 was identified as a candidate target of miR-137. Reporter assay with 3'UTR of Cox-2 cloned downstream of the luciferase gene showed reduced luciferase activity in the presence of miR-137, providing strong evidence that miR-137 was a direct regulator of Cox-2. Expression analysis further revealed that Cox-2 was elevated in glioma and associated with survival of patients. Furthermore, we observed that Cox-2 knockdown resulted in effects similar to those with miR-137 transfection in glioma cells. In conclusion, our study demonstrates that miR-137 deregulation is common in glioma, and restoration of its function inhibits cell proliferation and invasion, suggesting that miR-137 may act as a tumour suppressor.
LinkOut: [PMID: 22406049]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
13 hsa-miR-137 Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT002489 E2F6 E2F transcription factor 6 4 1
MIRT002490 CDK6 cyclin-dependent kinase 6 4 5
MIRT004004 NCOA2 nuclear receptor coactivator 2 4 1
MIRT004579 KDM1A lysine (K)-specific demethylase 1A 5 2
MIRT005485 CDC42 cell division cycle 42 (GTP binding protein, 25kDa) 4 3
MIRT005605 CTBP1 C-terminal binding protein 1 4 1
MIRT005883 MITF microphthalmia-associated transcription factor 3 1
MIRT006329 PTGS2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) 2 1
MIRT006957 ESRRA estrogen-related receptor alpha 1 1
MIRT007068 ZNF804A zinc finger protein 804A 1 1
MIRT007092 YBX1 Y box binding protein 1 1 1
MIRT007252 CSE1L CSE1 chromosome segregation 1-like (yeast) 2 1
MIRT007346 PXN paxillin 3 1