Accession ID: MIRT006501 [miRNA, hsa-miR-217 :: KRAS, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-217LinkOut: [miRBase ]
Synonyms MIRN217, mir-217, MIR217
Description Homo sapiens miR-217 stem-loop
Comment This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-217
Mature Sequence 35| UACUGCAUCAGGAACUGAUUGGA |57
Evidence Experimental
Experiments Cloned
Putative hsa-miR-217 Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol KRAS LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2
Description v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Transcript NM_033360    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on KRAS LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of KRAS
(miRNA target sites are highlighted)
>KRAS|NM_033360|3'UTR
   1 TCTGGGTGTTGATGATGCCTTCTATACATTAGTTCGAGAAATTCGAAAACATAAAGAAAAGATGAGCAAAGATGGTAAAA
  81 AGAAGAAAAAGAAGTCAAAGACAAAGTGTGTAATTATGTAAATACAATTTGTACTTTTTTCTTAAGGCATACTAGTACAA
 161 GTGGTAATTTTTGTACATTACACTAAATTATTAGCATTTGTTTTAGCATTACCTAATTTTTTTCCTGCTCCATGCAGACT
 241 GTTAGCTTTTACCTTAAATGCTTATTTTAAAATGACAGTGGAAGTTTTTTTTTCCTCTAAGTGCCAGTATTCCCAGAGTT
 321 TTGGTTTTTGAACTAGCAATGCCTGTGAAAAAGAAACTGAATACCTAAGATTTCTGTCTTGGGGTTTTTGGTGCATGCAG
 401 TTGATTACTTCTTATTTTTCTTACCAATTGTGAATGTTGGTGTGAAACAAATTAATGAAGCTTTTGAATCATCCCTATTC
 481 TGTGTTTTATCTAGTCACATAAATGGATTAATTACTAATTTCAGTTGAGACCTTCTAATTGGTTTTTACTGAAACATTGA
 561 GGGAACACAAATTTATGGGCTTCCTGATGATGATTCTTCTAGGCATCATGTCCTATAGTTTGTCATCCCTGATGAATGTA
 641 AAGTTACACTGTTCACAAAGGTTTTGTCTCCTTTCCACTGCTATTAGTCATGGTCACTCTCCCCAAAATATTATATTTTT
 721 TCTATAAAAAGAAAAAAATGGAAAAAAATTACAAGGCAATGGAAACTATTATAAGGCCATTTCCTTTTCACATTAGATAA
 801 ATTACTATAAAGACTCCTAATAGCTTTTCCTGTTAAGGCAGACCCAGTATGAAATGGGGATTATTATAGCAACCATTTTG
 881 GGGCTATATTTACATGCTACTAAATTTTTATAATAATTGAAAAGATTTTAACAAGTATAAAAAATTCTCATAGGAATTAA
 961 ATGTAGTCTCCCTGTGTCAGACTGCTCTTTCATAGTATAACTTTAAATCTTTTCTTCAACTTGAGTCTTTGAAGATAGTT
1041 TTAATTCTGCTTGTGACATTAAAAGATTATTTGGGCCAGTTATAGCTTATTAGGTGTTGAAGAGACCAAGGTTGCAAGGC
1121 CAGGCCCTGTGTGAACCTTTGAGCTTTCATAGAGAGTTTCACAGCATGGACTGTGTCCCCACGGTCATCCAGTGTTGTCA
1201 TGCATTGGTTAGTCAAAATGGGGAGGGACTAGGGCAGTTTGGATAGCTCAACAAGATACAATCTCACTCTGTGGTGGTCC
1281 TGCTGACAAATCAAGAGCATTGCTTTTGTTTCTTAAGAAAACAAACTCTTTTTTAAAAATTACTTTTAAATATTAACTCA
1361 AAAGTTGAGATTTTGGGGTGGTGGTGTGCCAAGACATTAATTTTTTTTTTAAACAATGAAGTGAAAAAGTTTTACAATCT
1441 CTAGGTTTGGCTAGTTCTCTTAACACTGGTTAAATTAACATTGCATAAACACTTTTCAAGTCTGATCCATATTTAATAAT
1521 GCTTTAAAATAAAAATAAAAACAATCCTTTTGATAAATTTAAAATGTTACTTATTTTAAAATAAATGAAGTGAGATGGCA
1601 TGGTGAGGTGAAAGTATCACTGGACTAGGAAGAAGGTGACTTAGGTTCTAGATAGGTGTCTTTTAGGACTCTGATTTTGA
1681 GGACATCACTTACTATCCATTTCTTCATGTTAAAAGAAGTCATCTCAAACTCTTAGTTTTTTTTTTTTACAACTATGTAA
1761 TTTATATTCCATTTACATAAGGATACACTTATTTGTCAAGCTCAGCACAATCTGTAAATTTTTAACCTATGTTACACCAT
1841 CTTCAGTGCCAGTCTTGGGCAAAATTGTGCAAGAGGTGAAGTTTATATTTGAATATCCATTCTCGTTTTAGGACTCTTCT
1921 TCCATATTAGTGTCATCTTGCCTCCCTACCTTCCACATGCCCCATGACTTGATGCAGTTTTAATACTTGTAATTCCCCTA
2001 ACCATAAGATTTACTGCTGCTGTGGATATCTCCATGAAGTTTTCCCACTGAGTCACATCAGAAATGCCCTACATCTTATT
2081 TCCTCAGGGCTCAAGAGAATCTGACAGATACCATAAAGGGATTTGACCTAATCACTAATTTTCAGGTGGTGGCTGATGCT
2161 TTGAACATCTCTTTGCTGCCCAATCCATTAGCGACAGTAGGATTTTTCAAACCTGGTATGAATAGACAGAACCCTATCCA
2241 GTGGAAGGAGAATTTAATAAAGATAGTGCTGAAAGAATTCCTTAGGTAATCTATAACTAGGACTACTCCTGGTAACAGTA
2321 ATACATTCCATTGTTTTAGTAACCAGAAATCTTCATGCAATGAAAAATACTTTAATTCATGAAGCTTACTTTTTTTTTTT
2401 GGTGTCAGAGTCTCGCTCTTGTCACCCAGGCTGGAATGCAGTGGCGCCATCTCAGCTCACTGCAACCTCCATCTCCCAGG
2481 TTCAAGCGATTCTCGTGCCTCGGCCTCCTGAGTAGCTGGGATTACAGGCGTGTGCCACTACACTCAACTAATTTTTGTAT
2561 TTTTAGGAGAGACGGGGTTTCACCCTGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAAGTGATTCACCCACCTTGGCC
2641 TCATAAACCTGTTTTGCAGAACTCATTTATTCAGCAAATATTTATTGAGTGCCTACCAGATGCCAGTCACCGCACAAGGC
2721 ACTGGGTATATGGTATCCCCAAACAAGAGACATAATCCCGGTCCTTAGGTAGTGCTAGTGTGGTCTGTAATATCTTACTA
2801 AGGCCTTTGGTATACGACCCAGAGATAACACGATGCGTATTTTAGTTTTGCAAAGAAGGGGTTTGGTCTCTGTGCCAGCT
2881 CTATAATTGTTTTGCTACGATTCCACTGAAACTCTTCGATCAAGCTACTTTATGTAAATCACTTCATTGTTTTAAAGGAA
2961 TAAACTTGATTATATTGTTTTTTTATTTGGCATAACTGTGATTCTTTTAGGACAATTACTGTACACATTAAGGTGTATGT
3041 CAGATATTCATATTGACCCAAATGTGTAATATTCCAGTTTTCTCTGCATAAGTAATTAAAATATACTTAAAAATTAATAG
3121 TTTTATCTGGGTACAAATAAACAGGTGCCTGAACTAGTTCACAGACAAGGAAACTTCTATGTAAAAATCACTATGATTTC
3201 TGAATTGCTATGTGAAACTACAGATCTTTGGAACACTGTTTAGGTAGGGTGTTAAGACTTACACAGTACCTCGTTTCTAC
3281 ACAGAGAAAGAAATGGCCATACTTCAGGAACTGCAGTGCTTATGAGGGGATATTTAGGCCTCTTGAATTTTTGATGTAGA
3361 TGGGCATTTTTTTAAGGTAGTGGTTAATTACCTTTATGTGAACTTTGAATGGTTTAACAAAAGATTTGTTTTTGTAGAGA
3441 TTTTAAAGGGGGAGAATTCTAGAAATAAATGTTACCTAATTATTACAGCCTTAAAGACAAAAATCCTTGTTGAAGTTTTT
3521 TTAAAAAAAGCTAAATTACATAGACTTAGGCATTAACATGTTTGTGGAAGAATATAGCAGACGTATATTGTATCATTTGA
3601 GTGAATGTTCCCAAGTAGGCATTCTAGGCTCTATTTAACTGAGTCACACTGCATAGGAATTTAGAACCTAACTTTTATAG
3681 GTTATCAAAACTGTTGTCACCATTGCACAATTTTGTCCTAATATATACATAGAAACTTTGTGGGGCATGTTAAGTTACAG
3761 TTTGCACAAGTTCATCTCATTTGTATTCCATTGATTTTTTTTTTCTTCTAAACATTTTTTCTTCAAACAGTATATAACTT
3841 TTTTTAGGGGATTTTTTTTTAGACAGCAAAAACTATCTGAAGATTTCCATTTGTCAAAAAGTAATGATTTCTTGATAATT
3921 GTGTAGTAATGTTTTTTAGAACCCAGCAGTTACCTTAAAGCTGAATTTATATTTAGTAACTTCTGTGTTAATACTGGATA
4001 GCATGAATTCTGCATTGAGAAACTGAATAGCTGTCATAAAATGAAACTTTCTTTCTAAAGAAAGATACTCACATGAGTTC
4081 TTGAAGAATAGTCATAACTAGATTAAGATCTGTGTTTTAGTTTAATAGTTTGAAGTGCCTGTTTGGGATAATGATAGGTA
4161 ATTTAGATGAATTTAGGGGAAAAAAAAGTTATCTGCAGATATGTTGAGGGCCCATCTCTCCCCCCACACCCCCACAGAGC
4241 TAACTGGGTTACAGTGTTTTATCCGAAAGTTTCCAATTCCACTGTCTTGTGTTTTCATGTTGAAAATACTTTTGCATTTT
4321 TCCTTTGAGTGCCAATTTCTTACTAGTACTATTTCTTAATGTAACATGTTTACCTGGAATGTATTTTAACTATTTTTGTA
4401 TAGTGTAAACTGAAACATGCACATTTTGTACATTGTGCTTTCTTTTGTGGGACATATGCAGTGTGATCCAGTTGTTTTCC
4481 ATCATTTGGTTGCGCTGACCTAGGAATGTTGGTCATATCAAACATTAAAAATGACCACTCTTTTAATTGAAATTAACTTT
4561 TAAATGTTTATAGGAGTATGTGCTGTGAAGTGATCTAAAATTTGTAATATTTTTGTCATGAACTGTACTACTCCTAATTA
4641 TTGTAATGTAATAAAAATAGTTACAGTGAC
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' agGUUAGUCAAGGACUACGUCAu 5'
            | || |   |:||||||||| 
Target 5' ccCCATGA---CTTGATGCAGTt 3'
1960 - 1979 158.00 -16.40
2
miRNA  3' agguuagUCAAGGAC----UACGUCAu 5'
                 :|||::||    ||||||| 
Target 5' gtcttggGGTTTTTGGTGCATGCAGTt 3'
376 - 402 154.00 -13.10
3
miRNA  3' agGUUAGUCAAGGAC--UACGUCAu 5'
            ||  |||   |||  ||||||| 
Target 5' gtCACCCAG--GCTGGAATGCAGTg 3'
2421 - 2443 151.00 -15.90
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-217 :: KRAS    [ Functional MTI ]
Validation Method Luciferase reporter assay , Reporter assay
Conditions PANC-1 , MIAPaCa-2 , AsPC-1 , BxPC-3 , P1 , P3 , P7 w
Disease pancreatic ductal adenocarcinoma;
Location of target site 3'UTR
Tools used in this research miRBase Target Database , miRTar , RNA22
Original Description (Extracted from the article) ... KRAS is a direct target of miR-217 ...

- Zhao, W. G. Yu, S. N. Lu, Z. H. Ma, Y. H. et al., 2010, Carcinogenesis.

Article - Zhao, W. G. Yu, S. N. Lu, Z. H. Ma, Y. H. et al.
- Carcinogenesis, 2010
Aberrantly expressed microRNA (miRNA) is frequently associated with a variety of cancers, including pancreatic ductal adenocarcinoma (PDAC). In this study, we investigated the expression and possible role of miR-217 in PDAC. Data obtained by locked nucleic acid in situ hybridization and real-time quantitative polymerase chain reaction showed that miR-217 was downregulated in 76.2% (16/21) of PDAC tissues and in all tested PDAC cell lines when compared with the corresponding normal pancreatic tissue. Overexpression of miR-217 in PDAC cells inhibited tumor cell growth and anchorage-independent colony formation and miR-217 decreased tumor cell growth in nude mouse xenografts in vivo. Using in silico predictions, KRAS was defined as a potential direct target of miR-217. Data from the dual-luciferase reporter gene assay showed that KRAS was a direct target of miR-217. Upregulation of miR-217 could decrease KRAS protein levels and reduce the constitutive phosphorylation of downstream AKT. Downregulation of miR-217 expression in PDAC cells could increase cell anchorage-independent colony formation and KRAS protein levels. Furthermore, miR-217 expression was observed to be negatively correlated with KRAS protein expression in PDAC cell lines. We conclude that the frequently downregulated miR-217 can regulate KRAS and function as a tumor suppressor in PDAC. Therefore, miR-217 may serve as a useful therapeutic agent for miRNA-based PDAC therapy.
LinkOut: [PMID: 20675343]
MiRNA-Target Expression Profile:

 
MiRNA-Target Expression Profile(TCGA):

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
67 hsa-miR-217 Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000144 SIRT1 sirtuin 1 4 2
MIRT000481 ROBO1 roundabout, axon guidance receptor, homolog 1 (Drosophila) 4 1
MIRT000533 PTEN phosphatase and tensin homolog 3 3
MIRT003535 NR4A2 nuclear receptor subfamily 4, group A, member 2 3 1
MIRT004010 EZH2 enhancer of zeste homolog 2 (Drosophila) 4 2
MIRT004866 TRAPPC2P1 trafficking protein particle complex 2 pseudogene 1 2 1
MIRT005685 PPM1D protein phosphatase, Mg2+/Mn2+ dependent, 1D 1 1
MIRT006363 FHIT fragile histidine triad 1 1
MIRT006501 KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog 1 1
MIRT007153 SMAD7 SMAD family member 7 1 1
MIRT024253 Pten phosphatase and tensin homolog 1 1
MIRT024254 IRS1 insulin receptor substrate 1 1 1
MIRT053154 E2F3 E2F transcription factor 3 2 1
MIRT054838 DACH1 dachshund homolog 1 (Drosophila) 3 1
MIRT097003 MAP1B microtubule-associated protein 1B 1 1
MIRT226550 TP53INP1 tumor protein p53 inducible nuclear protein 1 1 1
MIRT243450 LMLN leishmanolysin-like (metallopeptidase M8 family) 1 1
MIRT270975 FLVCR1 feline leukemia virus subgroup C cellular receptor 1 1 1
MIRT271119 ZNF268 zinc finger protein 268 1 2
MIRT315221 FOXO3 forkhead box O3 3 1
MIRT331090 GHITM growth hormone inducible transmembrane protein 1 1
MIRT349300 ZNF317 zinc finger protein 317 1 1
MIRT438314 GPC5 glypican 5 3 1
MIRT443492 ZNF583 zinc finger protein 583 1 1
MIRT445834 SRSF11 serine/arginine-rich splicing factor 11 1 1
MIRT462007 SNRNP27 small nuclear ribonucleoprotein 27kDa (U4/U6.U5) 1 1
MIRT488451 ACBD3 acyl-CoA binding domain containing 3 1 1
MIRT496632 TMEM67 transmembrane protein 67 1 1
MIRT502218 HSPA4L heat shock 70kDa protein 4-like 1 2
MIRT505421 TCF7L2 transcription factor 7-like 2 (T-cell specific, HMG-box) 1 2
MIRT508428 SFTPB surfactant protein B 1 1
MIRT513125 OSBPL3 oxysterol binding protein-like 3 1 1
MIRT521500 RAB11B RAB11B, member RAS oncogene family 1 1
MIRT532484 HOXA13 homeobox A13 1 1
MIRT533643 TMSB4X thymosin beta 4, X-linked 1 1
MIRT533938 TAF1D TATA box binding protein (TBP)-associated factor, RNA polymerase I, D, 41kDa 1 1
MIRT539384 ADSS adenylosuccinate synthase 1 3
MIRT546247 TNFRSF21 tumor necrosis factor receptor superfamily, member 21 1 1
MIRT547978 HIF1A hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) 1 1
MIRT550850 TMEM63A transmembrane protein 63A 1 1
MIRT555488 PNRC2 proline-rich nuclear receptor coactivator 2 1 1
MIRT558575 CREBZF CREB/ATF bZIP transcription factor 1 1
MIRT575909 March5 membrane-associated ring finger (C3HC4) 5 1 1
MIRT610752 MARCH5 membrane-associated ring finger (C3HC4) 5 1 3
MIRT613152 DNAL1 dynein, axonemal, light chain 1 1 1
MIRT624598 BACH2 BTB and CNC homology 1, basic leucine zipper transcription factor 2 1 1
MIRT630875 UBXN11 UBX domain protein 11 1 1
MIRT639314 CKAP4 cytoskeleton-associated protein 4 1 1
MIRT639870 STK40 serine/threonine kinase 40 1 1
MIRT641783 ZBTB20 zinc finger and BTB domain containing 20 1 1
MIRT645976 NHLRC2 NHL repeat containing 2 1 1
MIRT648474 TCERG1 transcription elongation regulator 1 1 1
MIRT648798 VPS8 vacuolar protein sorting 8 homolog (S. cerevisiae) 1 1
MIRT652845 TACO1 translational activator of mitochondrially encoded cytochrome c oxidase I 1 1
MIRT682748 CA6 carbonic anhydrase VI 1 1
MIRT683723 LACTB lactamase, beta 1 1
MIRT701707 MXD1 MAX dimerization protein 1 1 1
MIRT701913 MMGT1 membrane magnesium transporter 1 1 1
MIRT704818 CDC73 cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) 1 1
MIRT705207 BTG1 B-cell translocation gene 1, anti-proliferative 1 1
MIRT709727 FAM196B family with sequence similarity 196, member B 1 1
MIRT711160 WDR82P1 WD repeat domain 82 pseudogene 1 1 1
MIRT713022 SLC4A2 solute carrier family 4, anion exchanger, member 2 (erythrocyte membrane protein band 3-like 1) 1 1
MIRT718754 ZNF490 zinc finger protein 490 1 1
MIRT719493 LSG1 large subunit GTPase 1 homolog (S. cerevisiae) 1 1
MIRT720644 ELF5 E74-like factor 5 (ets domain transcription factor) 1 1
MIRT723026 HINFP histone H4 transcription factor 1 1
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