Accession ID: MIRT039125 [miRNA, hsa-miR-769-3p :: POLR3A, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-769LinkOut: [miRBase ]
Synonyms MIRN769, hsa-mir-769, MIR769
Description Homo sapiens miR-769 stem-loop
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-769-3p
Mature Sequence 69| CUGGGAUCUCCGGGGUCUUGGUU |91
Evidence Experimental
Experiments Cloned
Putative hsa-miR-769-3p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol POLR3A LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms RPC1, RPC155, hRPC155
Description polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
Transcript NM_0070    LinkOut: [ RefSeq ]
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on POLR3A LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of POLR3A
(miRNA target sites are highlighted)
>POLR3A|NM_0070|3'UTR
   1 TAGTCCAAAGAAAGAGGGGACCATGCCTGACCTTGACTCCTTGTCCTGTCTCCAGCTGATGTATAAAGAGTTTTGTGCTC
  81 CCTGGGACGGGGGTCCTGAGGTCCCCACCTATGCCAGCAATCAGAGAAGCCCTCTTGGCATCCCCAGGAGCAGCTTCTCC
 161 TCTGATAGGGTGCAGCTCACACCAGTGACCCTGACTGTGCCACGCTGCTCGGGAGAGCTGAGGGTTTTATTGTTTGCTTG
 241 CTTGAAACCTAATCTATAGACGGCCCCACAGCTCGTGCACACACTGCTTCCCTGGACTTAAAGCCCAGCCAGGTGTCATG
 321 GTCCATCCCAGCTCACTGCATACATCCATCGGCTCCCCATGGTGTCTTCACACCTGACGATGAGCCAGGCCTGAGCCCCA
 401 CACAGGCCAGGGCACATTCTTGGATTTTCCATTCCTTGGTCATGCTGGAATCTCTCAATGTGACATACTTATGTAAATAT
 481 TGTTACTATTATTTATTTGTTCCATTTGAGGGATTTGGAATTTTTGTTATTTTAGTTTTATTTTTGAAACCAAGCATCTA
 561 TAGAAACCAAGAAAGTCAGCATGTAAGCGTCACTGGAAAAACTGGTTTAAGCAAATAGAGCCGTCTGGGATTTGTAACTG
 641 AGGTGCAACTGTCATGAGGCCCAGGCAGCTCTGTAACATCTTCTATAGATGCCCCTGGCTACCCTGTTGTTTTCATCTAC
 721 CTCAGACCCCTATCATGGGGCTCTACCCTGTGACAAGAGCCAAACCCATTCTCCATGGCCTATGGAAGCCTCACTGGAGT
 801 TTGGGGCCTGCTGCAATGGGGATGAGATGGTTTTTTGTAGAATTATACTTACGTTCCTTGGATGATCTCTAGTTGATTTT
 881 TTAAGTTCTGAGTTGATGCTGTTAAGGTACCCGGGGTAGCCATTGGTTCTTGGATCTGTGTTAGAATGAGTGCTTTCCCT
 961 TCCTACTGATGTGATTGTGGATTAGGAATTCGTGACCGAGTGATTTTTGGCCAGTGGTTGGGTTTAAAATTCTATTAAAA
1041 TTTGTAGTTTGGGCTGGGTGCTGTGGCTCATACCTATAATCCCAGCACTTTGGGAGGCCGAGGGGGGCAGATCACTTGAG
1121 CCCAGGAGTTCGAGAGCAGCCTGGGCAACATGACGAAACCCCGTCTCTACAGAGAATACAAAAATTAGCCAGGCATGGTG
1201 GCCCATGCCTGTAGTACCTGCTATTGGTAAGGCTGAGGTGGAAGGTTCACTTGAGCCTGGGAGGTGGAGGTTGTAGTGAG
1281 CCAAGATCGTGCCACTACACTCCAGCCTGGGCAACAAAGCAAGACCTTGTCTCAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors  Predicted by miRanda
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-769-3p :: POLR3A    [ Functional MTI ]
Validation Method CLASH
Conditions Flp-In T-REx 293-hAGO1 cells
Location of target site CDS
Original Description (Extracted from the article) ... Validation of interactions identified by CLASH suppports their reliability. ...

- Helwak, A. Kudla, G. Dudnakova, T. Tollervey, D., 2013, Cell.

Article - Helwak, A. Kudla, G. Dudnakova, T. Tollervey, D.
- Cell, 2013
MicroRNAs (miRNAs) play key roles in gene regulation, but reliable bioinformatic or experimental identification of their targets remains difficult. To provide an unbiased view of human miRNA targets, we developed a technique for ligation and sequencing of miRNA-target RNA duplexes associated with human AGO1. Here, we report data sets of more than 18,000 high-confidence miRNA-mRNA interactions. The binding of most miRNAs includes the 5' seed region, but around 60% of seed interactions are noncanonical, containing bulged or mismatched nucleotides. Moreover, seed interactions are generally accompanied by specific, nonseed base pairing. 18% of miRNA-mRNA interactions involve the miRNA 3' end, with little evidence for 5' contacts, and some of these were functionally validated. Analyses of miRNA:mRNA base pairing showed that miRNA species systematically differ in their target RNA interactions, and strongly overrepresented motifs were found in the interaction sites of several miRNAs. We speculate that these affect the response of RISC to miRNA-target binding.
LinkOut: [PMID: 23622248]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
82 hsa-miR-769-3p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT039056 TMEM99 transmembrane protein 99 1 1
MIRT039057 PHAX phosphorylated adaptor for RNA export 1 1
MIRT039058 C1orf43 chromosome 1 open reading frame 43 1 1
MIRT039059 NME4 non-metastatic cells 4, protein expressed in 1 1
MIRT039060 PHF20L1 PHD finger protein 20-like 1 1 1
MIRT039061 KIF1C kinesin family member 1C 1 1
MIRT039062 NUP205 nucleoporin 205kDa 1 1
MIRT039063 ZNF598 zinc finger protein 598 1 1
MIRT039064 HIST2H4B histone cluster 2, H4b 1 1
MIRT039065 MXI1 MAX interactor 1 1 1
MIRT039066 NIPA2 non imprinted in Prader-Willi/Angelman syndrome 2 1 1
MIRT039067 PRKAR2A protein kinase, cAMP-dependent, regulatory, type II, alpha 1 1
MIRT039068 SIGMAR1 sigma non-opioid intracellular receptor 1 1 1
MIRT039069 STIM1 stromal interaction molecule 1 1 1
MIRT039070 KDELR1 KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1 1 1
MIRT039071 HMGCS1 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) 1 1
MIRT039072 EIF4A1 eukaryotic translation initiation factor 4A1 1 1
MIRT039073 RPL7L1 ribosomal protein L7-like 1 1 1
MIRT039074 ATXN7L3B ataxin 7-like 3B 1 1
MIRT039075 UBC ubiquitin C 1 1
MIRT039076 RPSA ribosomal protein SA 1 1
MIRT039077 EIF3L eukaryotic translation initiation factor 3, subunit L 1 1
MIRT039078 HOXA9 homeobox A9 1 1
MIRT039079 BRD2 bromodomain containing 2 1 1
MIRT039080 ISOC2 isochorismatase domain containing 2 1 1
MIRT039081 FAM3C family with sequence similarity 3, member C 1 1
MIRT039082 TMEM127 transmembrane protein 127 1 1
MIRT039083 SOD2 superoxide dismutase 2, mitochondrial 1 1
MIRT039084 PEG10 paternally expressed 10 1 1
MIRT039085 CTNNA1 catenin (cadherin-associated protein), alpha 1, 102kDa 1 1
MIRT039086 HIST2H3A histone cluster 2, H3a 1 1
MIRT039087 PAPD5 PAP associated domain containing 5 1 1
MIRT039088 RAI1 retinoic acid induced 1 1 1
MIRT039089 COL4A1 collagen, type IV, alpha 1 1 1
MIRT039090 GAPDH glyceraldehyde-3-phosphate dehydrogenase 1 1
MIRT039091 MAP3K5 mitogen-activated protein kinase kinase kinase 5 1 1
MIRT039092 TSPAN14 tetraspanin 14 1 1
MIRT039093 TBC1D9B TBC1 domain family, member 9B (with GRAM domain) 1 1
MIRT039094 AGO1 eukaryotic translation initiation factor 2C, 1 1 1
MIRT039095 RPL32 ribosomal protein L32 1 1
MIRT039096 PIP5K1A phosphatidylinositol-4-phosphate 5-kinase, type I, alpha 1 1
MIRT039097 ZNF395 zinc finger protein 395 1 1
MIRT039098 C19orf54 chromosome 19 open reading frame 54 1 1
MIRT039099 PHLDA3 pleckstrin homology-like domain, family A, member 3 1 1
MIRT039100 ADRM1 adhesion regulating molecule 1 1 1
MIRT039101 ELL elongation factor RNA polymerase II 1 1
MIRT039102 AXIN1 axin 1 1 1
MIRT039103 TACC3 transforming, acidic coiled-coil containing protein 3 1 1
MIRT039104 NDUFC2 NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa 1 1
MIRT039105 ZNF436 zinc finger protein 436 1 1
MIRT039106 MFSD12 chromosome 19 open reading frame 28 1 1
MIRT039107 TIAM1 T-cell lymphoma invasion and metastasis 1 1 1
MIRT039108 YY1 YY1 transcription factor 1 1
MIRT039109 RNASEK ribonuclease, RNase K 1 1
MIRT039110 INTS1 integrator complex subunit 1 1 1
MIRT039111 POM121 POM121 membrane glycoprotein (rat) 1 1
MIRT039112 EEF2 eukaryotic translation elongation factor 2 1 1
MIRT039113 PHKA1 phosphorylase kinase, alpha 1 (muscle) 1 1
MIRT039114 METTL13 methyltransferase like 13 1 1
MIRT039115 HNRNPH3 heterogeneous nuclear ribonucleoprotein H3 (2H9) 1 1
MIRT039116 SRRM2 serine/arginine repetitive matrix 2 1 1
MIRT039117 C11orf58 chromosome 11 open reading frame 58 1 1
MIRT039118 PLEC plectin 1 1
MIRT039119 ATP6V1F ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F 1 1
MIRT039120 CD81 CD81 molecule 1 1
MIRT039121 MBTPS1 membrane-bound transcription factor peptidase, site 1 1 1
MIRT039122 AP1B1 adaptor-related protein complex 1, beta 1 subunit 1 1
MIRT039123 ACLY ATP citrate lyase 1 1
MIRT039124 HOXA11 homeobox A11 1 1
MIRT039125 POLR3A polymerase (RNA) III (DNA directed) polypeptide A, 155kDa 1 1
MIRT039126 ANXA2 annexin A2 1 1
MIRT039127 TRIM37 tripartite motif-containing 37 1 1
MIRT039128 RPL13A ribosomal protein L13a 1 1
MIRT039129 CLCN7 chloride channel 7 1 1
MIRT039130 ESF1 ESF1, nucleolar pre-rRNA processing protein, homolog (S. cerevisiae) 1 1
MIRT039131 RNF4 ring finger protein 4 1 1
MIRT039132 TRAP1 TNF receptor-associated protein 1 1 1
MIRT039133 ACTG1 actin, gamma 1 1 1
MIRT039134 EMILIN3 elastin microfibril interfacer 3 1 1
MIRT039135 RPL23A ribosomal protein L23a 1 1
MIRT039136 ZFAND5 zinc finger, AN1-type domain 5 1 1
MIRT039137 DLX4 distal-less homeobox 4 1 1