Accession ID: MIRT039602 [miRNA, hsa-miR-616-5p :: ATP7B, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-616LinkOut: [miRBase ]
Synonyms MIRN616, hsa-mir-616, MIR616
Description Homo sapiens miR-616 stem-loop
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-616-5p
Mature Sequence 16| ACUCAAAACCCUUCAGUGACUU |37
Evidence Experimental
Experiments SAGE
Putative hsa-miR-616-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol ATP7B LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms PWD, WC1, WD, WND
Description ATPase, Cu++ transporting, beta polypeptide
Transcript NM_0000    LinkOut: [ RefSeq ]
Other Transcripts NM_0010059   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on ATP7B LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of ATP7B
(miRNA target sites are highlighted)
>ATP7B|NM_0000|3'UTR
   1 TGATGACTTCAGGCAGGCGGGCCGGGGCAGGGACTTGCCTCCACTCACCACAAGCTGAGCAGGACAGCCAGCAGCAGGAT
  81 GGGCTGAGCTAGCCTCCAGCTTTGGGGACTTCCGCTCCCTGGATATGTCCAGTCATCCTGCCCTGCAGCACGCGGCCTTG
 161 TCTGGGTGCAGCTGGGCTTGGCCTGGAGAGGACGGCCCTGCCTGCCTCTTGGCCTCACGGGACCGTCAGCATGGGCTTTG
 241 TCTTGGACTCTAGTCCTTGGCTGGACTGTAGAAGGTGAGAGGCGAGTCACCCTCCTCACAGACCTCTGCTTGGAGTATTT
 321 AGGATGACTGCTGTGAAATGGAGAACAGTTTCATCAGGACCAAAAAACCTCACTGGGCCTTTCCAGAGAACTGCAGACCT
 401 CACTGTCAGGGTCTTTCTGATGACGCCTGTCTGTGTGCATCATGTTTCTGAGACCACAGTTTACCTCAGGTGTGCCTGTT
 481 GCTTTCTTCCTGCATAGTCTGTTCCTTTCTTCGTACATAGTCTGTTCCTTTTCTCTCCTGTGTGCTTGTCAGTGGGGACC
 561 CCTCGCAACCCTGCCTGTCACCTGGGAGGGTGGGACCAATGTCCTTGTGGTCTTTGCTGCTGCTCTCAGGCGCTTCTCCA
 641 ATGCTCTGGAGTGTGCATTTCAGCTTGAACCTGCTTCCTGGCTCACACATCCCCAGCCAGGGAGCTTGCCACACTCTTCT
 721 TCAAGTTGAGGAGAGTTCTTTTTTGCTTAAAGCCCCCTTCTCCATGGAGTGTTGGCTTCTCAATAGAGTGTTGTTGCTGA
 801 CCAGCTGGAGTGAGGGCCTCAGAGCCTGACCTGAGAGTCCGTACTCGGCTTCCTGTGGGGTGTAGGTTCTCGCGATTCAG
 881 GACGTCCTTCCATATCCCTGCCCAGCCTGTGGTGCTTGAAACGTTTGCCCCATGGGAAACGTATGTGTGCAGGAGCCTCC
 961 CTGCACGGCCCAAGGGGCTTCGTTTTCAGTCTTCTGACTGTCACCTCGTGGGGTTCAGTAGAGAATTCATGTGACTAGCG
1041 CCTGGCCTTGTGTGGCTTGGAGGAAATGGTACTGCCCAAATAGGAGGAAAACACAGCCTCCCTGAGCCTGCATTCTGCAC
1121 GCTGCCCAGGGGCTTCAGAAAAGGAGTGGCCACAGCACCCCGAAGGGAGCATCTGTTTACCTGGCAGTGGCTCTCAGAGC
1201 AGCAGAACGGGTTCAGTTTTAGACTCTGAAGTTGGTTGTGATTGACAGAACCCTTTGGGAGCAAACTAGTAGAGTTGGAT
1281 TAAATTCTGGGTGAAACCCTTTTCTCCCACACAAAATAGTTTTAGTGATTTTTTTCATTGTCCATTACTTGCCAGGGGCA
1361 GTTTTAGCAGCACTTTTGATAGATTACGTCTAATCCTCCCAACCAACCAGCAGGGTAGCTATTACTGTCCACATTTTACA
1441 GGCAAGGAAACAGGCTCCAAGAGGCTGAGGACTTTGCCCAGGATGACATAGCCAATGGACAAGCAGTGTCTGTCAGCTGT
1521 GAAGGCTTCACTCTTATTGTCCTTCTACCTTGAATAGAAGTTTTCCTGATAAGAATAAACGAGGAAAAGGTCCTTGCCTC
1601 CTGGAAGAACAAATCTACCAGGTGATCTATTCATTGTTTCAACTCAGAATGCACTTGATTCAGGAGGTCATCTGACCTTC
1681 ACCTTGGATGGTTAGTTTCACTTTTTACATATAGTTTTTGCAGGGTTTTATTTTATAAAATCCAAGCGCGCTGTTGATTG
1761 TGTTTTCCTTGTTTTCAGCCCCCCCACTCCAGCCCGCAGCACATTTCCGCTGTCCGTCAGTAATTGTGTCCTCTCTTTAT
1841 GCTTGCTTGGGGAATGTTGTTTTCTGACTAGGCTGATCATTATCTAAAGAATCTAATTCTGTTGATTTTTAAAACTTTTA
1921 GGACCATAAACGTTGTGTTCATATATGGACATGGAAATATTTATATAATTTTATAGAAAATAACCTTTTAGATGGTCAAA
2001 GTGTAAGGAGTTTTTTTGTCAGATAATCATTTCTACTTCAAAAACATTTCATGCAATATTAGAATAAAGTTCCTGTCATT
2081 CCTCTAAAAAAA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' uuCAGUGACUUC--CCAAAACUca 5'
            | | ||||||  |||| |||  
Target 5' taGACTCTGAAGTTGGTTGTGAtt 3'
1220 - 1243 122.00 -11.70
2
miRNA  3' uucaGUGAC---UUCC------CAAAACUCa 5'
              ||| |   ||||      ||||| || 
Target 5' cctgCACGGCCCAAGGGGCTTCGTTTTCAGt 3'
960 - 990 120.00 -10.00
3
miRNA  3' uucAGUGACUUCCCAAAAC-UCa 5'
             |:||  |  | ||||| || 
Target 5' tttTTACATATAGTTTTTGCAGg 3'
1702 - 1724 119.00 -7.69
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-616-5p :: ATP7B    [ Functional MTI ]
Validation Method CLASH
Conditions Flp-In T-REx 293-hAGO1 cells
Location of target site CDS
Original Description (Extracted from the article) ... Validation of interactions identified by CLASH suppports their reliability. ...

- Helwak, A. Kudla, G. Dudnakova, T. Tollervey, D., 2013, Cell.

miRNA-target interactions (Provided by authors)
IDDuplex structurePosition
1
miRNA  3' ttcagtgacttCCCAAAACTCa 5'
                     |||||||||| 
Target 5' ----------tGGGTTTTGAGg 3'
1 - 12
Article - Helwak, A. Kudla, G. Dudnakova, T. Tollervey, D.
- Cell, 2013
MicroRNAs (miRNAs) play key roles in gene regulation, but reliable bioinformatic or experimental identification of their targets remains difficult. To provide an unbiased view of human miRNA targets, we developed a technique for ligation and sequencing of miRNA-target RNA duplexes associated with human AGO1. Here, we report data sets of more than 18,000 high-confidence miRNA-mRNA interactions. The binding of most miRNAs includes the 5' seed region, but around 60% of seed interactions are noncanonical, containing bulged or mismatched nucleotides. Moreover, seed interactions are generally accompanied by specific, nonseed base pairing. 18% of miRNA-mRNA interactions involve the miRNA 3' end, with little evidence for 5' contacts, and some of these were functionally validated. Analyses of miRNA:mRNA base pairing showed that miRNA species systematically differ in their target RNA interactions, and strongly overrepresented motifs were found in the interaction sites of several miRNAs. We speculate that these affect the response of RISC to miRNA-target binding.
LinkOut: [PMID: 23622248]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
2 hsa-miR-616-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT039602 ATP7B ATPase, Cu++ transporting, beta polypeptide 1 1
MIRT039603 DRG2 developmentally regulated GTP binding protein 2 1 1