Accession ID: MIRT045314 [miRNA, hsa-miR-185-5p :: PDPK1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-185LinkOut: [miRBase ]
Synonyms MIRN185, miR-185, MIR185
Description Homo sapiens miR-185 stem-loop
Comment This miRNA sequence is predicted based on homology to a verified miRNA from mouse .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-185-5p
Mature Sequence 15| UGGAGAGAAAGGCAGUUCCUGA |36
Evidence Experimental
Experiments Cloned
Putative hsa-miR-185-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol PDPK1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms MGC20087, MGC35290, PDK1, PRO0461
Description 3-phosphoinositide dependent protein kinase-1
Transcript NM_0026    LinkOut: [ RefSeq ]
Other Transcripts NM_0312   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on PDPK1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of PDPK1
(miRNA target sites are highlighted)
>PDPK1|NM_0026|3'UTR
   1 TGACGTGGCCTGCGGCCGGGCTGCCCTTCGCTGCCAGGACACCTGCCCCAGCGCGGCTTGGCCGCCATCCGGGACGCTTC
  81 CAGACCACCTGCCAGCCATCACAAGGGGAACGCAGAGGCGGAAACCTTGCAGCATTTTTATTTAAAAGAAAAGAAGAAAA
 161 AAAACACCCAACCACACAAAGAACAAAACCAGTAACAAACACAAAGGAATTCAGGGTCGCTTTGCTTGCTCTCTGTGCTC
 241 CGTGGAGGCCTCCGTGTGCCCTCGTTGCCGTGGGGACCCAGCTCCATGCACGTCAACCCAGTCCCGCCCAGACTAGTGGA
 321 CAGACCTGGTGTCACCAGTTTTTCCTAGCATCAGTCCGAACCATGCGCCCGCCCTGCCCCAACTGTGTGCTGGTCCTGCT
 401 GTGGCCGAGGGGACCGGGTGTGTTTGGCTCTTTATGCCCCTCCCGCTGTGGTCCTGGAACTCTTCACCAGGGAGGGAGCC
 481 CTGCGGGGGCCGCAGCTTTGTGGAGGGAGCCGCCGTGCTTCTGTCACCTGCTCCCTTTCTTGCGTCTCCCTGTGATGGGC
 561 CCTTAGGCCTGGCTGGGCCCATTACATATCCCTGTGGTGGCTCTGGTGGCAGCTTTCTGTGGCCCCTGCTGTGTTGGCAG
 641 GCAGGTTTGCGTGGTGAGGAGCGGGAGGGGTTGGAGTGGTGCGGGAGCAGGCTGCCGAGTGGAGGGTGCCATCGAGGGCT
 721 CCGGATCCCTTATCCTACTTAGCAGTGTTGGTCTCTGGGGCTGGAAGCCGAGCGCATGCTGGGAGCGGTACTGTCAGAAG
 801 TGAGCCCAGTTAGTACCCCGCTGGCTCACTGCACGAGAGAGTCCTGCCCCGAGCCCTAGGTGGGGCCAGGAGGTGCCTTG
 881 GAGAAGCCAGCCAGAGCAGAGAGGGCTGCTGACTTCCGTGTGGAGCAGAGAGGCCTGAGGGCCTCCTAAAAGGTTTAAAT
 961 GTCCACGCCTCTCCAGTTGCTGAAGTAGGGTCTGAGAGAACCCTGGCATCAGCAGACCCAGGGTGCTTCTGTCTCCTGCA
1041 GACCACGCCAGGGAGTGCAGACACCACCGTCACACACGCCCCTTTTGTGTTTTGGTTCAAGTTTCTCAGAGCCCCTCAGA
1121 GCTTCTACATCTGTGCATCAGAAATCTCACAGCCTTCTCATGCTGCCGGCTCATCTGGGCCCATAGAGTGGGCTTTGCCA
1201 GTTGCTGTTGCACAGGAGGCGAGAACAGCACACTTCAACCCCAGCTTGCTGGTCGGCTTTCCTCTAGAGAGAGCCGGTTT
1281 TGGGGCCATTTCCCTTTGATGCTTTGGTGGCCTTGCCCCGCTCTGCAGCACAGACAGGCCAGATGCATTTGTCCTTTGCC
1361 TAGCTACTCCCCAGGTAGAGAGTGCTCCTGGTGGCCTGGCAGGTCTGGGCCCTTCTCTCCCTGCCCAGGTTGTCCCTGGA
1441 GGGCAGCCCTCACTCCCTTTGGGGGAGAGGCAGACATTGCTGCCCACAGACCTGCCTCTGACTCAACTGTGTCCACCCTC
1521 CCTGGTCCCTACCCCCAAGTCACAGGTGACTCAGCAGTGACCCTGTGTGCCAGGCCAGATCCAAACTGAGAGGGAAGGTG
1601 TCGTTTTTACACTGCTAATGACGAGAGTGGCTCTTTTTAGCTAGGCGAGTACAGACGGGGCCTGGGAGGGGGCAGAGATG
1681 TTCCCCAGGCCCTGCCTGTGGTTCCTGCCTGGGCCTTGGCTGCTGCTGTGTGAGAGCTGCATGTGAGCCTGTGACCGTGA
1761 GCTGGGGTGAGCTGGGCCGCACCTACCCTGGGGCCCCAGGGAGCAGGACGCTCCGGGGCCCAGCACGTTGCCCTGGGCCT
1841 GTGGCCGGAGTCGGAGTCCTCTCTCCTCCTCCTGGCTTTTGGAAAGGCTTGGCTGTGTTGGGGAGTCTCTCTTAGCCCTT
1921 TCAGGAATTTCTGTTCAGGCTTCCTCCTCCTCATCAGCTATTTTACCCATCTCAGAACGTCCTGTGTCTCCATGTAGGAG
2001 AGTGGCTCTCTCAGATCTCTCAGGGCGTCTGGTTATAGGGAAACAAGTGGAGCAGGGACGTGGCTTTAATTGGAGCACTC
2081 GGCTGGGCTGCTTGGGGAGACTCTTCCGTGCGTTCTTCCTCTGGATAGAACCACCACCTCCTGGGCGTCACTGACAAGCT
2161 CCATCTTAACCTCCAAAGCCACAGAACTAGGGGCTCAGAGCCAGAGCTGGCAGCCGCCAGCCAAAATGATGCCATTGCCT
2241 GAGCTGACAGCCAAGCCCTTCTGTGGGTCACCTTTCTCCTCACCCAGCCCCTTGCTCTTCCCTTTTGAAAGGCCCGTGTG
2321 TTTTCTTTCCTTACCCTGTGCTTGCTCATGTCTACTCCGGTTTTCTCTACCACATCCTTAGAGCCATCACCTGGCACGCA
2401 GGCGCCTTACATTCTACGGTAGAACGTGGGGTACTGTGTGTGCACATAGACACACTTACGTGGAATTACAGTTGTGGGTT
2481 TATCCAAGATGAGGAAGATTTCACCTGCTGTTTAATAGACTTGGGGCCATGTGCCTCCCCACACATGGGCAAGGACAGGT
2561 GGAATGTCGGGACCACACTGTGCGGCTTCTCGGCACAAAGCGGAGGGAGGCTGTGGTCGCTGCCGGCCTAGGTGTCCCAG
2641 GTGCCCCGCCTTTCTCTGGGACACAGTTGGGGGCTGGCTTCTGAGGGATTCCTTTCTCCCCTCTTTGTGTGGCCCCAGCC
2721 AGGGCGGTGGGCAGTCCTGGTGTAGAGCACAAGCCTCTCCACCCTAGAGAAATGCCTCTGTACCACGGCTACCATGTGGA
2801 ACCTTAACTTGCAGAAGGCTTGTTAACAATTGTTTTGAGAGAGATGGCTGGTCATGCCACAGCTGCTGGGGACTCCGCCT
2881 ACTCCAGCCCTCTTGGGACACACTGTGGGATTTGTGGCCCTTCCCCAGAGGAATTGTGGAGACTGTCCCATGGAACAAAC
2961 CCTCAGGCACCAGCACAGGGCTCTGGGTGACTCAGTAAAACTAACGTTTGTCTCTGACAAGATCAGCTGTAGGCTCACCG
3041 GCCAGAGAAGACCACTGTGAGCATTTTGCCGTATATCCTGCCCTGCCATTTGTTCACTTTTTAAACTAAAATAGGAACAT
3121 CCGACACACACCGTTTGCATCGTCTTCTCCCTTGATATTTTAAGCATTTTCCCATGTCATGAGTTTCTCAGAAACATGTT
3201 TTTAACAATTGTACTATTTAGTCATTGTCCATTTACTATAATTTATCTGACCATTTCCCTACTGTAAAATACTTAAGACG
3281 GTTTCTGATTTTTCCACTATTTAAATAATGCTGTGATGAATATCTTTAAAATCTTCTGATTTCTTACTTTTTTCCCCCTT
3361 AGATGCCTGGAAGTGGTATTTTGAGGTGAAAGAGTTTGTTCATTTTGAAGATATTTCTGTCTCTCTCTCGACCTGATGTG
3441 TAGACGCTCACTTCCAGTAGCAGAACCACCTTAGTTGTGTCTTACAGATTCTGAACAAATCGGTTTCTGATAAGCCATGT
3521 GTTCCAAAGAATGTCTGAATAAGACCGCTCTTTATTTAAATGCTAAGAGGATGTCACTACTGCAATCCATCTGTGGCCGA
3601 TTTTTTCCAAGAGCCAATTTCCTTGTTTTGGTTGCAAGAACCTGGCTCTGCCTGCATGTCAGCTCTCTGCCCTCCCTGCT
3681 GCCGTGGCTTTCAAGCGCTTGGCAGAATCTTGTACTTCGTGTCCACAATGGTACTGAATTTGCATCTGCACAGTCAGCAG
3761 AGATAACAAGTGTTGAACTGACCTTGCCACATGCTTAGTGAGTGATTTGTAATTAAGTTTATAGACTCAGAAGGTATATT
3841 AGGACATTTGGAATCAGTAGCAGAGCAAAGCCTCTTTGAAAAAAACCACGTAGCTGATTGGGTTTTACAAGAGTGCATTT
3921 GTCTCCCCCTTCCACCCGTGGGGCCCCACCTTCAGGTCTTAGTGGTTCACAAGAGCCCAGCAGCCAGGCTGGCTTTTTCA
4001 TTGTAGGGCGTGGTTGTCCCAGCTGGTGTAGATTTCAGGCCGCCCCCCCCAACTCCCTGCCCACAGTGTTGCAGATTGCC
4081 TGGCTGGCAGCAAGTCCAGACCACCCAAATTTGGTTGGATTCTTCATTTCTCCACTGTAGTTGGGGTCCATTGATTGTGC
4161 AGGGGAACGTGCAGGAGGTTTTTCTAGGCACCGTGTTCAGTGCTGCTTCACTCTACCAGAGATTATGGCCAAATTGCACG
4241 GAATTTGGTTTCTTGCCCTCTGAAGCCTGAGGGCCCCCCCTTGCCTGGCTGGTTGACAGACCCGGGGTGGTCACTGCTGA
4321 GACTTCAGAGATCGCAGCTGCTGTGAGAATACGGTGAAGGTACTTTGTTCTGGAAGATGTTGTCATACACTTTTCCCCAG
4401 TTATTTTCAAACTTGACATGAGCCTATGTTGACTCACTGGGTGGGGGTCCCTTCTTACGCAGCACACGTGGCAAGTGCCT
4481 GAATCGGGGCTGGAGGCACTTCAGAGCCTCTGAGGGGCCACCACTTCTGGCCCAAAATTGCAGGGTTGTAGATGAGGCTG
4561 CCTGTGGAGAACTGGTGTGAGGAGGAAGCTGTTTCCAACAAAGAGCACTTTCATCTGTTGAGATGGCTGTGGTGAGCAAC
4641 TGAACGAGCCTACGTGTGTACCTGAATTTTCCCCGTAACTCATTTCTTCCATATGAAGAAACACCAAACTATGTACAGAG
4721 AACTTTTTACAAAAGGCAGACCTTTTTTAAGCTGTGTAACCCACATAGCCTAACCACCTGGCAGAATGACTACGAATAGG
4801 GGTCATTGTGCTGGTAAAAGCCTCTATTACGACTGTAAGTAAGTTGGATGTTGGCAAAATTAAATTGTTACAGTATTTAG
4881 AGCTGCTGTAGCTGTTCCTTCACAACATAAAATAGGATAAATGACTAGTACGTCTTTCAGGTGGGTGGCAAGCAGAACAT
4961 GCGTAATATTCTCTACCTGGTCTGTAGCTGTAACTGTGATGTACAGACAAAGCAAAAATTAAAAGAACTTATGAAAACAA
5041 ATGCAATGATACTAGGATATACACTTTTGTATTTTTATTCTTATATAAGGTTATTTGCTGGCTATTGTTGGCCTCTAGTT
5121 CAGTCTGTGTTATTTAAATTCTAATATATGAATTATTTGAATTGAATTCATGTTCGGGGCCACGTTGTTGTATGTATTGA
5201 TGTACAGCCTTGAATGTGAATAATTATTGTAAACTATATTTTACAACTTTTTTTCTGGCTTTATTATATAAATTTTCTAT
5281 TGGGTCAGTGATTTAATCATATAATTTAATGAATCTGTTTATCCTTTTTTTTTTTCCAAATACTTGTGCTTTAGGTGTAG
5361 TTACCAGATGATGAATTTTCCTCGTATGGTCAGTAGTCTTGTAATAAAAAGCATGTAGAGTGTAAAAAAAAAAAAAAAAA
5441 AA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' agUCCUUGA--CGGAAAGAGAGGu 5'
            |||  ||  ||| |||||||| 
Target 5' gcAGG-TCTGGGCCCTTCTCTCCc 3'
1399 - 1421 161.00 -23.20
2
miRNA  3' aguCCUUGACGGAAAGAGAGGu 5'
             |||::  ||  ||||||| 
Target 5' gtcGGAGT--CC--TCTCTCCt 3'
1850 - 1867 147.00 -15.00
3
miRNA  3' agUCCUUGACGGAAAGAGAGGu 5'
            |||:|:| |||||||| || 
Target 5' tgAGGGATT-CCTTTCTCCCCt 3'
2682 - 2702 147.00 -21.70
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-185-5p :: PDPK1    [ Functional MTI ]
Validation Method CLASH
Conditions Flp-In T-REx 293-hAGO1 cells
Location of target site 3'UTR
Original Description (Extracted from the article) ... Validation of interactions identified by CLASH suppports their reliability. ...

- Helwak, A. Kudla, G. Dudnakova, T. Tollervey, D., 2013, Cell.

miRNA-target interactions (Provided by authors)
IDDuplex structurePosition
1
miRNA  3' agtccttgacggaaAGAGAGGt 5'
                        ||||||| 
Target 5' ------------ccTCTCTCCt 3'
1 - 10
Article - Helwak, A. Kudla, G. Dudnakova, T. Tollervey, D.
- Cell, 2013
MicroRNAs (miRNAs) play key roles in gene regulation, but reliable bioinformatic or experimental identification of their targets remains difficult. To provide an unbiased view of human miRNA targets, we developed a technique for ligation and sequencing of miRNA-target RNA duplexes associated with human AGO1. Here, we report data sets of more than 18,000 high-confidence miRNA-mRNA interactions. The binding of most miRNAs includes the 5' seed region, but around 60% of seed interactions are noncanonical, containing bulged or mismatched nucleotides. Moreover, seed interactions are generally accompanied by specific, nonseed base pairing. 18% of miRNA-mRNA interactions involve the miRNA 3' end, with little evidence for 5' contacts, and some of these were functionally validated. Analyses of miRNA:mRNA base pairing showed that miRNA species systematically differ in their target RNA interactions, and strongly overrepresented motifs were found in the interaction sites of several miRNAs. We speculate that these affect the response of RISC to miRNA-target binding.
LinkOut: [PMID: 23622248]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
88 hsa-miR-185-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004036 RHOA ras homolog gene family, member A 4 1
MIRT004037 CDC42 cell division cycle 42 (GTP binding protein, 25kDa) 4 1
MIRT004698 CCNE1 cyclin E1 3 1
MIRT004700 CDK6 cyclin-dependent kinase 6 4 2
MIRT004701 AKT1 v-akt murine thymoma viral oncogene homolog 1 3 1
MIRT004702 HMGA2 high mobility group AT-hook 2 3 1
MIRT004703 CORO2B coronin, actin binding protein, 2B 3 1
MIRT005550 SIX1 SIX homeobox 1 4 1
MIRT005963 NTRK3 neurotrophic tyrosine kinase, receptor, type 3 1 1
MIRT006696 DNMT1 DNA (cytosine-5-)-methyltransferase 1 4 1
MIRT006962 EPAS1 endothelial PAS domain protein 1 2 1
MIRT035517 AR androgen receptor 1 1
MIRT035549 SCARB1 scavenger receptor class B, member 1 1 1
MIRT045312 MATR3 matrin 3 1 1
MIRT045313 NACC1 nucleus accumbens associated 1, BEN and BTB (POZ) domain containing 1 1
MIRT045314 PDPK1 3-phosphoinositide dependent protein kinase-1 1 1
MIRT045315 SREBF1 sterol regulatory element binding transcription factor 1 1 1
MIRT045316 PPP1CC protein phosphatase 1, catalytic subunit, gamma isozyme 1 1
MIRT045317 MSANTD1 chromosome 4 open reading frame 44 1 1
MIRT045318 WDR77 WD repeat domain 77 1 1
MIRT045319 MSL3 male-specific lethal 3 homolog (Drosophila) 1 1
MIRT045320 ARL9 ADP-ribosylation factor-like 9 1 1
MIRT045321 AADACL3 arylacetamide deacetylase-like 3 1 1
MIRT045322 FASTK Fas-activated serine/threonine kinase 1 1
MIRT045323 COPB1 coatomer protein complex, subunit beta 1 1 1
MIRT045324 STK35 serine/threonine kinase 35 1 1
MIRT045325 HSP90AA1 heat shock protein 90kDa alpha (cytosolic), class A member 1 1 1
MIRT045326 CAMKK2 calcium/calmodulin-dependent protein kinase kinase 2, beta 1 1
MIRT045327 FRMPD4 FERM and PDZ domain containing 4 1 1
MIRT045328 PHACTR2 phosphatase and actin regulator 2 1 1
MIRT045329 CYFIP1 cytoplasmic FMR1 interacting protein 1 1 1
MIRT045330 GLUL glutamate-ammonia ligase 1 1
MIRT045331 RAPH1 Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 1 1
MIRT045332 DENND2A DENN/MADD domain containing 2A 1 1
MIRT045333 GLB1L3 galactosidase, beta 1-like 3 1 1
MIRT045334 CANX calnexin 1 1
MIRT045335 GSTA4 glutathione S-transferase alpha 4 1 1
MIRT045336 ANKHD1 ankyrin repeat and KH domain containing 1 1 1
MIRT045337 MPV17 MpV17 mitochondrial inner membrane protein 1 1
MIRT045338 EEF2 eukaryotic translation elongation factor 2 1 1
MIRT045339 UBR4 ubiquitin protein ligase E3 component n-recognin 4 1 1
MIRT045340 TUBB tubulin, beta 1 1
MIRT045341 NFE2L1 nuclear factor (erythroid-derived 2)-like 1 1 1
MIRT045342 DIAPH1 diaphanous homolog 1 (Drosophila) 1 1
MIRT045343 KIAA0368 KIAA0368 1 1
MIRT045344 SMG7 Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) 1 1
MIRT045345 UBA52 ubiquitin A-52 residue ribosomal protein fusion product 1 1 1
MIRT045346 AHCYL2 adenosylhomocysteinase-like 2 1 1
MIRT045347 TMEM160 transmembrane protein 160 1 1
MIRT045348 EIF4H eukaryotic translation initiation factor 4H 1 1
MIRT045349 ATP5G2 ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C2 (subunit 9) 1 1
MIRT045350 LDHB lactate dehydrogenase B 1 1
MIRT045351 EEF1A1 eukaryotic translation elongation factor 1 alpha 1 1 1
MIRT045352 KITLG KIT ligand 1 1
MIRT045353 MED17 mediator complex subunit 17 1 1
MIRT045354 GCN1L1 GCN1 general control of amino-acid synthesis 1-like 1 (yeast) 1 1
MIRT045355 LENG8 leukocyte receptor cluster (LRC) member 8 1 1
MIRT045356 CACHD1 cache domain containing 1 1 1
MIRT045357 DAZAP1 DAZ associated protein 1 1 1
MIRT045358 RAB1B RAB1B, member RAS oncogene family 1 1
MIRT045359 GLUD1 glutamate dehydrogenase 1 1 1
MIRT045360 LUZP1 leucine zipper protein 1 1 1
MIRT045361 AGO1 eukaryotic translation initiation factor 2C, 1 1 1
MIRT045362 RAE1 RAE1 RNA export 1 homolog (S. pombe) 1 1
MIRT045363 SMG6 Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) 1 1
MIRT045364 ACTN4 actinin, alpha 4 1 1
MIRT045365 PBX2 pre-B-cell leukemia homeobox 2 1 1
MIRT045366 UHMK1 U2AF homology motif (UHM) kinase 1 1 1
MIRT045367 SCD stearoyl-CoA desaturase (delta-9-desaturase) 1 1
MIRT045368 GOLGA2P5 golgin A2 family, member B 1 1
MIRT045369 DNAJC8 DnaJ (Hsp40) homolog, subfamily C, member 8 1 1
MIRT045370 ASH1L ash1 (absent, small, or homeotic)-like (Drosophila) 1 1
MIRT045371 WNT5B wingless-type MMTV integration site family, member 5B 1 1
MIRT045372 MARS methionyl-tRNA synthetase 1 1
MIRT045373 AKT1S1 AKT1 substrate 1 (proline-rich) 1 1
MIRT045374 MLF1IP MLF1 interacting protein 1 1
MIRT045375 AP3M1 adaptor-related protein complex 3, mu 1 subunit 1 1
MIRT045376 CTTN cortactin 1 1
MIRT045377 SET SET nuclear oncogene 1 1
MIRT045378 IBTK inhibitor of Bruton agammaglobulinemia tyrosine kinase 1 1
MIRT045379 XPO6 exportin 6 1 1
MIRT045380 GPR108 G protein-coupled receptor 108 1 1
MIRT045381 TRRAP transformation/transcription domain-associated protein 1 1
MIRT045382 ARL16 ADP-ribosylation factor-like 16 1 1
MIRT045383 C16orf70 chromosome 16 open reading frame 70 1 1
MIRT045384 PLEKHB2 pleckstrin homology domain containing, family B (evectins) member 2 1 1
MIRT045385 EMC10 chromosome 19 open reading frame 63 1 1
MIRT045386 APEX1 APEX nuclease (multifunctional DNA repair enzyme) 1 1 1