Accession ID: MIRT045314 [miRNA, hsa-miR-185-5p :: PDPK1, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-185LinkOut: [miRBase ]
Synonyms MIRN185, miR-185, MIR185
Description Homo sapiens miR-185 stem-loop
Comment This miRNA sequence is predicted based on homology to a verified miRNA from mouse .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-185-5p
Mature Sequence 15| UGGAGAGAAAGGCAGUUCCUGA |36
Evidence Experimental
Experiments Cloned
Putative hsa-miR-185-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol PDPK1 LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms PDK1, PRO0461
Description 3-phosphoinositide dependent protein kinase-1
Transcript NM_0026    LinkOut: [ RefSeq ]
Other Transcripts NM_0312   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on PDPK1 LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of PDPK1
(miRNA target sites are highlighted)
>PDPK1|NM_0026|3'UTR
   1 CGTGGCCTGCGGCCGGGCTGCCCTTCGCTGCCAGGACACCTGCCCCAGCGCGGCTTGGCCGCCATCCGGGACGCTTCCAG
  81 ACCACCTGCCAGCCATCACAAGGGGAACGCAGAGGCGGAAACCTTGCAGCATTTTTATTTAAAAGAAAAGAAGAAAAAAA
 161 ACACCCAACCACACAAAGAACAAAACCAGTAACAAACACAAAGGAATTCAGGGTCGCTTTGCTTGCTCTCTGTGCTCCGT
 241 GGAGGCCTCCGTGTGCCCTCGTTGCCGTGGGGACCCAGCTCCATGCACGTCAACCCAGTCCCGCCCAGACTAGTGGACAG
 321 ACCTGGTGTCACCAGTTTTTCCTAGCATCAGTCCGAACCATGCGCCCGCCCTGCCCCAACTGTGTGCTGGTCCTGCTGTG
 401 GCCGAGGGGACCGGGTGTGTTTGGCTCTTTATGCCCCTCCCGCTGTGGTCCTGGAACTCTTCACCAGGGAGGGAGCCCTG
 481 CGGGGGCCGCAGCTTTGTGGAGGGAGCCGCCGTGCTTCTGTCACCTGCTCCCTTTCTTGCGTCTCCCTGTGATGGGCCCT
 561 TAGGCCTGGCTGGGCCCATTACATATCCCTGTGGTGGCTCTGGTGGCAGCTTTCTGTGGCCCCTGCTGTGTTGGCAGGCA
 641 GGTTTGCGTGGTGAGGAGCGGGAGGGGTTGGAGTGGTGCGGGAGCAGGCTGCCGAGTGGAGGGTGCCATCGAGGGCTCCG
 721 GATCCCTTATCCTACTTAGCAGTGTTGGTCTCTGGGGCTGGAAGCCGAGCGCATGCTGGGAGCGGTACTGTCAGAAGTGA
 801 GCCCAGTTAGTACCCCGCTGGCTCACTGCACGAGAGAGTCCTGCCCCGAGCCCTAGGTGGGGCCAGGAGGTGCCTTGGAG
 881 AAGCCAGCCAGAGCAGAGAGGGCTGCTGACTTCCGTGTGGAGCAGAGAGGCCTGAGGGCCTCCTAAAAGGTTTAAATGTC
 961 CACGCCTCTCCAGTTGCTGAAGTAGGGTCTGAGAGAACCCTGGCATCAGCAGACCCAGGGTGCTTCTGTCTCCTGCAGAC
1041 CACGCCAGGGAGTGCAGACACCACCGTCACACACGCCCCTTTTGTGTTTTGGTTCAAGTTTCTCAGAGCCCCTCAGAGCT
1121 TCTACATCTGTGCATCAGAAATCTCACAGCCTTCTCATGCTGCCGGCTCATCTGGGCCCATAGAGTGGGCTTTGCCAGTT
1201 GCTGTTGCACAGGAGGCGAGAACAGCACACTTCAACCCCAGCTTGCTGGTCGGCTTTCCTCTAGAGAGAGCCGGTTTTGG
1281 GGCCATTTCCCTTTGATGCTTTGGTGGCCTTGCCCCGCTCTGCAGCACAGACAGGCCAGATGCATTTGTCCTTTGCCTAG
1361 CTACTCCCCAGGTAGAGAGTGCTCCTGGTGGCCTGGCAGGTCTGGGCCCTTCTCTCCCTGCCCAGGTTGTCCCTGGAGGG
1441 CAGCCCTCACTCCCTTTGGGGGAGAGGCAGACATTGCTGCCCACAGACCTGCCTCTGACTCAACTGTGTCCACCCTCCCT
1521 GGTCCCTACCCCCAAGTCACAGGTGACTCAGCAGTGACCCTGTGTGCCAGGCCAGATCCAAACTGAGAGGGAAGGTGTCG
1601 TTTTTACACTGCTAATGACGAGAGTGGCTCTTTTTAGCTAGGCGAGTACAGACGGGGCCTGGGAGGGGGCAGAGATGTTC
1681 CCCAGGCCCTGCCTGTGGTTCCTGCCTGGGCCTTGGCTGCTGCTGTGTGAGAGCTGCATGTGAGCCTGTGACCGTGAGCT
1761 GGGGTGAGCTGGGCCGCACCTACCCTGGGGCCCCAGGGAGCAGGACGCTCCGGGGCCCAGCACGTTGCCCTGGGCCTGTG
1841 GCCGGAGTCGGAGTCCTCTCTCCTCCTCCTGGCTTTTGGAAAGGCTTGGCTGTGTTGGGGAGTCTCTCTTAGCCCTTTCA
1921 GGAATTTCTGTTCAGGCTTCCTCCTCCTCATCAGCTATTTTACCCATCTCAGAACGTCCTGTGTCTCCATGTAGGAGAGT
2001 GGCTCTCTCAGATCTCTCAGGGCGTCTGGTTATAGGGAAACAAGTGGAGCAGGGACGTGGCTTTAATTGGAGCACTCGGC
2081 TGGGCTGCTTGGGGAGACTCTTCCGTGCGTTCTTCCTCTGGATAGAACCACCACCTCCTGGGCGTCACTGACAAGCTCCA
2161 TCTTAACCTCCAAAGCCACAGAACTAGGGGCTCAGAGCCAGAGCTGGCAGCCGCCAGCCAAAATGATGCCATTGCCTGAG
2241 CTGACAGCCAAGCCCTTCTGTGGGTCACCTTTCTCCTCACCCAGCCCCTTGCTCTTCCCTTTTGAAAGGCCCGTGTGTTT
2321 TCTTTCCTTACCCTGTGCTTGCTCATGTCTACTCCGGTTTTCTCTACCACATCCTTAGAGCCATCACCTGGCACGCAGGC
2401 GCCTTACATTCTACGGTAGAACGTGGGGTACTGTGTGTGCACATAGACACACTTACGTGGAATTACAGTTGTGGGTTTAT
2481 CCAAGATGAGGAAGATTTCACCTGCTGTTTAATAGACTTGGGGCCATGTGCCTCCCCACACATGGGCAAGGACAGGTGGA
2561 ATGTCGGGACCACACTGTGCGGCTTCTCGGCACAAAGCGGAGGGAGGCTGTGGTCGCTGCCGGCCTAGGTGTCCCAGGTG
2641 CCCCGCCTTTCTCTGGGACACAGTTGGGGGCTGGCTTCTGAGGGATTCCTTTCTCCCCTCTTTGTGTGGCCCCAGCCAGG
2721 GCGGTGGGCAGTCCTGGTGTAGAGCACAAGCCTCTCCACCCTAGAGAAATGCCTCTGTACCACGGCTACCATGTGGAACC
2801 TTAACTTGCAGAAGGCTTGTTAACAATTGTTTTGAGAGAGATGGCTGGTCATGCCACAGCTGCTGGGGACTCCGCCTACT
2881 CCAGCCCTCTTGGGACACACTGTGGGATTTGTGGCCCTTCCCCAGAGGAATTGTGGAGACTGTCCCATGGAACAAACCCT
2961 CAGGCACCAGCACAGGGCTCTGGGTGACTCAGTAAAACTAACGTTTGTCTCTGACAAGATCAGCTGTAGGCTCACCGGCC
3041 AGAGAAGACCACTGTGAGCATTTTGCCGTATATCCTGCCCTGCCATTTGTTCACTTTTTAAACTAAAATAGGAACATCCG
3121 ACACACACCGTTTGCATCGTCTTCTCCCTTGATATTTTAAGCATTTTCCCATGTCATGAGTTTCTCAGAAACATGTTTTT
3201 AACAATTGTACTATTTAGTCATTGTCCATTTACTATAATTTATCTGACCATTTCCCTACTGTAAAATACTTAAGACGGTT
3281 TCTGATTTTTCCACTATTTAAATAATGCTGTGATGAATATCTTTAAAATCTTCTGATTTCTTACTTTTTTCCCCCTTAGA
3361 TGCCTGGAAGTGGTATTTTGAGGTGAAAGAGTTTGTTCATTTTGAAGATATTTCTGTCTCTCTCTCGACCTGATGTGTAG
3441 ACGCTCACTTCCAGTAGCAGAACCACCTTAGTTGTGTCTTACAGATTCTGAACAAATCGGTTTCTGATAAGCCATGTGTT
3521 CCAAAGAATGTCTGAATAAGACCGCTCTTTATTTAAATGCTAAGAGGATGTCACTACTGCAATCCATCTGTGGCCGATTT
3601 TTTCCAAGAGCCAATTTCCTTGTTTTGGTTGCAAGAACCTGGCTCTGCCTGCATGTCAGCTCTCTGCCCTCCCTGCTGCC
3681 GTGGCTTTCAAGCGCTTGGCAGAATCTTGTACTTCGTGTCCACAATGGTACTGAATTTGCATCTGCACAGTCAGCAGAGA
3761 TAACAAGTGTTGAACTGACCTTGCCACATGCTTAGTGAGTGATTTGTAATTAAGTTTATAGACTCAGAAGGTATATTAGG
3841 ACATTTGGAATCAGTAGCAGAGCAAAGCCTCTTTGAAAAAAACCACGTAGCTGATTGGGTTTTACAAGAGTGCATTTGTC
3921 TCCCCCTTCCACCCGTGGGGCCCCACCTTCAGGTCTTAGTGGTTCACAAGAGCCCAGCAGCCAGGCTGGCTTTTTCATTG
4001 TAGGGCGTGGTTGTCCCAGCTGGTGTAGATTTCAGGCCGCCCCCCCCAACTCCCTGCCCACAGTGTTGCAGATTGCCTGG
4081 CTGGCAGCAAGTCCAGACCACCCAAATTTGGTTGGATTCTTCATTTCTCCACTGTAGTTGGGGTCCATTGATTGTGCAGG
4161 GGAACGTGCAGGAGGTTTTTCTAGGCACCGTGTTCAGTGCTGCTTCACTCTACCAGAGATTATGGCCAAATTGCACGGAA
4241 TTTGGTTTCTTGCCCTCTGAAGCCTGAGGGCCCCCCCTTGCCTGGCTGGTTGACAGACCCGGGGTGGTCACTGCTGAGAC
4321 TTCAGAGATCGCAGCTGCTGTGAGAATACGGTGAAGGTACTTTGTTCTGGAAGATGTTGTCATACACTTTTCCCCAGTTA
4401 TTTTCAAACTTGACATGAGCCTATGTTGACTCACTGGGTGGGGGTCCCTTCTTACGCAGCACACGTGGCAAGTGCCTGAA
4481 TCGGGGCTGGAGGCACTTCAGAGCCTCTGAGGGGCCACCACTTCTGGCCCAAAATTGCAGGGTTGTAGATGAGGCTGCCT
4561 GTGGAGAACTGGTGTGAGGAGGAAGCTGTTTCCAACAAAGAGCACTTTCATCTGTTGAGATGGCTGTGGTGAGCAACTGA
4641 ACGAGCCTACGTGTGTACCTGAATTTTCCCCGTAACTCATTTCTTCCATATGAAGAAACACCAAACTATGTACAGAGAAC
4721 TTTTTACAAAAGGCAGACCTTTTTTAAGCTGTGTAACCCACATAGCCTAACCACCTGGCAGAATGACTACGAATAGGGGT
4801 CATTGTGCTGGTAAAAGCCTCTATTACGACTGTAAGTAAGTTGGATGTTGGCAAAATTAAATTGTTACAGTATTTAGAGC
4881 TGCTGTAGCTGTTCCTTCACAACATAAAATAGGATAAATGACTAGTACGTCTTTCAGGTGGGTGGCAAGCAGAACATGCG
4961 TAATATTCTCTACCTGGTCTGTAGCTGTAACTGTGATGTACAGACAAAGCAAAAATTAAAAGAACTTATGAAAACAAATG
5041 CAATGATACTAGGATATACACTTTTGTATTTTTATTCTTATATAAGGTTATTTGCTGGCTATTGTTGGCCTCTAGTTCAG
5121 TCTGTGTTATTTAAATTCTAATATATGAATTATTTGAATTGAATTCATGTTCGGGGCCACGTTGTTGTATGTATTGATGT
5201 ACAGCCTTGAATGTGAATAATTATTGTAAACTATATTTTACAACTTTTTTTCTGGCTTTATTATATAAATTTTCTATTGG
5281 GTCAGTGATTTAATCATATAATTTAATGAATCTGTTTATCCTTTTTTTTTTTCCAAATACTTGTGCTTTAGGTGTAGTTA
5361 CCAGATGATGAATTTTCCTCGTATGGTCAGTAGTCTTGTAATAAAAAGCATGTAGAGTGTA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' agUCCUUGA--CGGAAAGAGAGGu 5'
            |||  ||  ||| |||||||| 
Target 5' gcAGG-TCTGGGCCCTTCTCTCCc 3'
1396 - 1418 161.00 -23.20
2
miRNA  3' aguCCUUGACGGAAAGAGAGGu 5'
             |||::  ||  ||||||| 
Target 5' gtcGGAGT--CC--TCTCTCCt 3'
1847 - 1864 147.00 -15.00
3
miRNA  3' agUCCUUGACGGAAAGAGAGGu 5'
            |||:|:| |||||||| || 
Target 5' tgAGGGATT-CCTTTCTCCCCt 3'
2679 - 2699 147.00 -21.70
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-185-5p :: PDPK1    [ Functional MTI ]
Validation Method CLASH
Conditions Flp-In T-REx 293-hAGO1 cells
Location of target site 3'UTR
Original Description (Extracted from the article) ... Validation of interactions identified by CLASH suppports their reliability. ...

- Helwak, A. Kudla, G. Dudnakova, T. Tollervey, D., 2013, Cell.

miRNA-target interactions (Provided by authors)
IDDuplex structurePosition
1
miRNA  3' agtccttgacggaaAGAGAGGt 5'
                        ||||||| 
Target 5' ------------ccTCTCTCCt 3'
1 - 10
Article - Helwak, A. Kudla, G. Dudnakova, T. Tollervey, D.
- Cell, 2013
MicroRNAs (miRNAs) play key roles in gene regulation, but reliable bioinformatic or experimental identification of their targets remains difficult. To provide an unbiased view of human miRNA targets, we developed a technique for ligation and sequencing of miRNA-target RNA duplexes associated with human AGO1. Here, we report data sets of more than 18,000 high-confidence miRNA-mRNA interactions. The binding of most miRNAs includes the 5' seed region, but around 60% of seed interactions are noncanonical, containing bulged or mismatched nucleotides. Moreover, seed interactions are generally accompanied by specific, nonseed base pairing. 18% of miRNA-mRNA interactions involve the miRNA 3' end, with little evidence for 5' contacts, and some of these were functionally validated. Analyses of miRNA:mRNA base pairing showed that miRNA species systematically differ in their target RNA interactions, and strongly overrepresented motifs were found in the interaction sites of several miRNAs. We speculate that these affect the response of RISC to miRNA-target binding.
LinkOut: [PMID: 23622248]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
88 hsa-miR-185-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004036 RHOA ras homolog family member A 4 1
MIRT004037 CDC42 cell division cycle 42 (GTP binding protein, 25kDa) 4 1
MIRT004698 CCNE1 cyclin E1 3 1
MIRT004700 CDK6 cyclin-dependent kinase 6 4 2
MIRT004701 AKT1 v-akt murine thymoma viral oncogene homolog 1 3 1
MIRT004702 HMGA2 high mobility group AT-hook 2 3 1
MIRT004703 CORO2B coronin, actin binding protein, 2B 3 1
MIRT005550 SIX1 SIX homeobox 1 4 1
MIRT005963 NTRK3 neurotrophic tyrosine kinase, receptor, type 3 1 1
MIRT006696 DNMT1 DNA (cytosine-5-)-methyltransferase 1 4 1
MIRT006962 EPAS1 endothelial PAS domain protein 1 2 1
MIRT035517 AR androgen receptor 1 1
MIRT035549 SCARB1 scavenger receptor class B, member 1 1 1
MIRT045312 MATR3 matrin 3 1 1
MIRT045313 NACC1 nucleus accumbens associated 1, BEN and BTB (POZ) domain containing 1 1
MIRT045314 PDPK1 3-phosphoinositide dependent protein kinase-1 1 1
MIRT045315 SREBF1 sterol regulatory element binding transcription factor 1 1 1
MIRT045316 PPP1CC protein phosphatase 1, catalytic subunit, gamma isozyme 1 1
MIRT045317 MSANTD1 Myb/SANT-like DNA-binding domain containing 1 1 1
MIRT045318 WDR77 WD repeat domain 77 1 1
MIRT045319 MSL3 male-specific lethal 3 homolog (Drosophila) 1 1
MIRT045320 ARL9 ADP-ribosylation factor-like 9 1 1
MIRT045321 AADACL3 arylacetamide deacetylase-like 3 1 1
MIRT045322 FASTK Fas-activated serine/threonine kinase 1 1
MIRT045323 COPB1 coatomer protein complex, subunit beta 1 1 1
MIRT045324 STK35 serine/threonine kinase 35 1 1
MIRT045325 HSP90AA1 heat shock protein 90kDa alpha (cytosolic), class A member 1 1 1
MIRT045326 CAMKK2 calcium/calmodulin-dependent protein kinase kinase 2, beta 1 1
MIRT045327 FRMPD4 FERM and PDZ domain containing 4 1 1
MIRT045328 PHACTR2 phosphatase and actin regulator 2 1 1
MIRT045329 CYFIP1 cytoplasmic FMR1 interacting protein 1 1 1
MIRT045330 GLUL glutamate-ammonia ligase 1 1
MIRT045331 RAPH1 Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 1 1
MIRT045332 DENND2A DENN/MADD domain containing 2A 1 1
MIRT045333 GLB1L3 galactosidase, beta 1-like 3 1 1
MIRT045334 CANX calnexin 1 1
MIRT045335 GSTA4 glutathione S-transferase alpha 4 1 1
MIRT045336 ANKHD1 ankyrin repeat and KH domain containing 1 1 1
MIRT045337 MPV17 MpV17 mitochondrial inner membrane protein 1 1
MIRT045338 EEF2 eukaryotic translation elongation factor 2 1 1
MIRT045339 UBR4 ubiquitin protein ligase E3 component n-recognin 4 1 1
MIRT045340 TUBB tubulin, beta class I 1 1
MIRT045341 NFE2L1 nuclear factor (erythroid-derived 2)-like 1 1 1
MIRT045342 DIAPH1 diaphanous homolog 1 (Drosophila) 1 1
MIRT045343 KIAA0368 KIAA0368 1 1
MIRT045344 SMG7 smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) 1 1
MIRT045345 UBA52 ubiquitin A-52 residue ribosomal protein fusion product 1 1 1
MIRT045346 AHCYL2 adenosylhomocysteinase-like 2 1 1
MIRT045347 TMEM160 transmembrane protein 160 1 1
MIRT045348 EIF4H eukaryotic translation initiation factor 4H 1 1
MIRT045349 ATP5G2 ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C2 (subunit 9) 1 1
MIRT045350 LDHB lactate dehydrogenase B 1 1
MIRT045351 EEF1A1 eukaryotic translation elongation factor 1 alpha 1 1 1
MIRT045352 KITLG KIT ligand 1 1
MIRT045353 MED17 mediator complex subunit 17 1 1
MIRT045354 GCN1L1 GCN1 general control of amino-acid synthesis 1-like 1 (yeast) 1 1
MIRT045355 LENG8 leukocyte receptor cluster (LRC) member 8 1 1
MIRT045356 CACHD1 cache domain containing 1 1 1
MIRT045357 DAZAP1 DAZ associated protein 1 1 1
MIRT045358 RAB1B RAB1B, member RAS oncogene family 1 1
MIRT045359 GLUD1 glutamate dehydrogenase 1 1 1
MIRT045360 LUZP1 leucine zipper protein 1 1 1
MIRT045361 AGO1 eukaryotic translation initiation factor 2C, 1 1 1
MIRT045362 RAE1 RAE1 RNA export 1 homolog (S. pombe) 1 1
MIRT045363 SMG6 smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) 1 1
MIRT045364 ACTN4 actinin, alpha 4 1 1
MIRT045365 PBX2 pre-B-cell leukemia homeobox 2 1 1
MIRT045366 UHMK1 U2AF homology motif (UHM) kinase 1 1 1
MIRT045367 SCD stearoyl-CoA desaturase (delta-9-desaturase) 1 1
MIRT045368 GOLGA2P5 golgin A2 pseudogene 5 1 1
MIRT045369 DNAJC8 DnaJ (Hsp40) homolog, subfamily C, member 8 1 1
MIRT045370 ASH1L ash1 (absent, small, or homeotic)-like (Drosophila) 1 1
MIRT045371 WNT5B wingless-type MMTV integration site family, member 5B 1 1
MIRT045372 MARS methionyl-tRNA synthetase 1 1
MIRT045373 AKT1S1 AKT1 substrate 1 (proline-rich) 1 1
MIRT045374 MLF1IP MLF1 interacting protein 1 1
MIRT045375 AP3M1 adaptor-related protein complex 3, mu 1 subunit 1 1
MIRT045376 CTTN cortactin 1 1
MIRT045377 SET SET nuclear oncogene 1 1
MIRT045378 IBTK inhibitor of Bruton agammaglobulinemia tyrosine kinase 1 1
MIRT045379 XPO6 exportin 6 1 1
MIRT045380 GPR108 G protein-coupled receptor 108 1 1
MIRT045381 TRRAP transformation/transcription domain-associated protein 1 1
MIRT045382 ARL16 ADP-ribosylation factor-like 16 1 1
MIRT045383 C16orf70 chromosome 16 open reading frame 70 1 1
MIRT045384 PLEKHB2 pleckstrin homology domain containing, family B (evectins) member 2 1 1
MIRT045385 EMC10 ER membrane protein complex subunit 10 1 1
MIRT045386 APEX1 APEX nuclease (multifunctional DNA repair enzyme) 1 1 1