Accession ID: MIRT050713 [miRNA, hsa-miR-18a-5p :: LARGE, target gene]
pre-miRNA Information
pre-miRNA ID hsa-mir-18aLinkOut: [miRBase ]
Synonyms MIR18, MIRN18, MIRN18A, hsa-mir-18, hsa-mir-18a, miR-18, miRNA18A, MIR18A
Description Homo sapiens miR-18a stem-loop
Comment This sequence maps to chromosome 13 and is named miR-18 precursor-13 in reference .
2nd Structure of pre-miRNA
Disease
Mature miRNA Information
Mature miRNA hsa-miR-18a-5p
Mature Sequence 6| UAAGGUGCAUCUAGUGCAGAUAG |28
Evidence Experimental
Experiments Cloned
Putative hsa-miR-18a-5p Targets LinkOut: [ TargetScanS 5.1 | MicroCosm | microRNA.org | miRecords | miRDB | miRo | miRNAMap 2.0 ]
Gene Information
Gene Symbol LARGE LinkOut: [ Entrez Gene | BioGPS | Wikipedia | iHop ]
Synonyms KIAA0609, MDC1D
Description like-glycosyltransferase
Transcript NM_0047    LinkOut: [ RefSeq ]
Other Transcripts NM_1336   
Expression LinkOut: [ BioGPS ]
Putative miRNA Targets on LARGE LinkOut: [ TargetScan 5.1 | MicroCosm | miRNAMap 2.0 ]
3'UTR of LARGE
(miRNA target sites are highlighted)
>LARGE|NM_0047|3'UTR
   1 TAGCACCAAGAAGCCCACCACTAGGGGGAGACATGCTGTAGGGGAAGTGCCACTCGCTGTTTGGGGCCCGGCCTTCAAAT
  81 TCAAAATTGAGCCATGCTTTTTCGGTTTGTTTTTATTTATCTCTTTGGCCCAGCCAAGCTGCCCTCACTACAGAGACCTT
 161 GGACAAGGATCCAGCCAGTCCCTCTCTGCCCCACAACCCTGCATTCCCAGAGGTTAGCTATGCAGCCCACCTAGATGAGT
 241 CTCTTCAAGAATGGGAAATCAAGGGGTGACAGGGAGTAAAAGGGTTATCATCTTACTGCAAAGCCACAAGATCAGGGCAG
 321 GGCTTTAGGATGTTCTGGATGCTTTTTAATAATTATGCTTCCCATCATAACTGGGGAGAAAGGGAAGTCAGGGTTCTAGG
 401 GGTTATTCGTCCCAGGAAATAGAAGTGAAATTGTCTTTATTAAGTGAAAACTTTCCCCTTTGCCCTGCAATGTAGCTGGG
 481 CATTCAAACGGAGGGCAAACCGATGATCTAAACCAACCACTTGGAAAAACCCAATGGGGACATTGTAACCAGAGGGTCCT
 561 GGAGGTGGGGTTGATGGGTTTCCTTATCCCCAAAGTCACTCCTGTTTTGTTTTGTTTTTCTTTGGGGGTTTTGTTTATTT
 641 TTGGGGCTGGCAATCCAAAATAGAAAATCTGATCCTTTGAGGCTCTAAAGGAAAATCAGCTGCCTCTACCAACCACCCTC
 721 TATCAGCAGTGGCCCAGGAAGGAGGTCAAGCATCTTCGGCCGATATTTAAACATGGGCAGCTTCCTTCAGGATGATCACC
 801 GAGGCTCCCGTGACTTTGAACTCCCTACTCTCCAGAATCCAGGGGCTATAGCGATGGGGACTGCGGAATTACGAGGGCTG
 881 GCTGTTTTACACCGGTCACATTTTCTATTGGCAGTGACTGATTCATGGGAAAGGGCTTTGAAGGAACTACTTCAGTGCAC
 961 ACACAAGGTACGAACCTCTCAGGCCTTTCGAAGAACTTTCATAATTCATGAAAGCCCAGTTCTGAAGATTCACGTATCCA
1041 TCTGGAGACCTACAGGAAGAAAGTGATTGGGTTCCTCTGGTTCTTGCCTGCTTCACTGTGGATGGGAAGAGGTGACAACC
1121 TCAGTCTCCCTTTGGGACCTGTCCAAGGGTAGGCAACCACCTTCACCTTCACACAGATTGAGGAGACACTGGACTTTTTA
1201 CCCATTTTCTTTAATCTTCAATATTAATATTGTGTTTACATTGATGAGAACAAGAGTTAATGCCCTACCCTCTGCTGGGC
1281 TGTTTGTATTGAGTTGCAATGTGACCAGCGAAAGCTGCATTTAATAAATGAAAGTACAGACTGTTTCTTCCCCA
Target sites Provided by authors  Predicted by miRanda
miRNA-target interactions (Predicted by miRanda)
IDDuplex structurePositionScoreMFE
1
miRNA  3' gauagaCGUGAUCUA---CGUGGAAu 5'
                | |  ||:|   |||:||| 
Target 5' gcccagGAAGGAGGTCAAGCATCTTc 3'
732 - 757 129.00 -8.00
2
miRNA  3' gaUAGACGUG-AUCUACGUGGAAu 5'
            ||:|  || |:|  ||| ||| 
Target 5' atATTTAAACATGG--GCAGCTTc 3'
763 - 784 121.00 -7.20
3
miRNA  3' gauagaCG-UGAUCUACGUGGAAu 5'
                || || |  | |||||| 
Target 5' gggtagGCAACCACCTTCACCTTc 3'
1147 - 1170 121.00 -9.40
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target hsa-miR-18a-5p :: LARGE    [ Functional MTI ]
Validation Method CLASH
Conditions Flp-In T-REx 293-hAGO1 cells
Location of target site CDS
Original Description (Extracted from the article) ... Validation of interactions identified by CLASH suppports their reliability. ...

- Helwak, A. Kudla, G. Dudnakova, T. Tollervey, D., 2013, Cell.

Article - Helwak, A. Kudla, G. Dudnakova, T. Tollervey, D.
- Cell, 2013
MicroRNAs (miRNAs) play key roles in gene regulation, but reliable bioinformatic or experimental identification of their targets remains difficult. To provide an unbiased view of human miRNA targets, we developed a technique for ligation and sequencing of miRNA-target RNA duplexes associated with human AGO1. Here, we report data sets of more than 18,000 high-confidence miRNA-mRNA interactions. The binding of most miRNAs includes the 5' seed region, but around 60% of seed interactions are noncanonical, containing bulged or mismatched nucleotides. Moreover, seed interactions are generally accompanied by specific, nonseed base pairing. 18% of miRNA-mRNA interactions involve the miRNA 3' end, with little evidence for 5' contacts, and some of these were functionally validated. Analyses of miRNA:mRNA base pairing showed that miRNA species systematically differ in their target RNA interactions, and strongly overrepresented motifs were found in the interaction sites of several miRNAs. We speculate that these affect the response of RISC to miRNA-target binding.
LinkOut: [PMID: 23622248]
MiRNA-Target Expression Profile:

 
MiRNA-Target Interaction Network:
Strong evidence (reporter assay, western blot, qRT-PCR or qPCR)
Other evidence
168 hsa-miR-18a-5p Target Genes:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT000703 ESR1 estrogen receptor 1 5 3
MIRT000792 IGF2BP2 insulin-like growth factor 2 mRNA binding protein 2 1 1
MIRT003370 PTEN phosphatase and tensin homolog 4 1
MIRT004174 CTGF connective tissue growth factor 4 3
MIRT004311 NCOA3 nuclear receptor coactivator 3 2 1
MIRT004383 CCNL1 cyclin L1 2 1
MIRT004384 SPPL3 signal peptide peptidase 3 1 1
MIRT004385 CSRNP3 cysteine-serine-rich nuclear protein 3 2 1
MIRT004386 THRA thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian) 2 1
MIRT004387 TNFSF11 tumor necrosis factor (ligand) superfamily, member 11 3 1
MIRT004545 NR3C1 nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) 3 1
MIRT004547 TSC22D3 TSC22 domain family, member 3 2 1
MIRT004569 BCL2L11 BCL2-like 11 (apoptosis facilitator) 1 2
MIRT004587 HIF1A hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) 1 2
MIRT004592 TGFBR2 transforming growth factor, beta receptor II (70/80kDa) 3 2
MIRT004665 Prmt5 protein arginine methyltransferase 5 4 1
MIRT004666 Myc myelocytomatosis oncogene 4 1
MIRT004736 SMAD4 SMAD family member 4 5 1
MIRT005361 HSF2 heat shock transcription factor 2 4 1
MIRT006118 ATM ataxia telangiectasia mutated 4 3
MIRT006613 Smad2 MAD homolog 2 (Drosophila) 3 1
MIRT006614 Smad4 MAD homolog 4 (Drosophila) 3 1
MIRT006817 NEDD9 neural precursor cell expressed, developmentally down-regulated 9 2 1
MIRT006818 CDK19 cyclin-dependent kinase 19 2 1
MIRT007178 DICER1 dicer 1, ribonuclease type III 5 2
MIRT007323 SMAD3 SMAD family member 3 1 1
MIRT031310 THBS1 thrombospondin 1 1 1
MIRT031311 ZFAND5 zinc finger, AN1-type domain 5 1 1
MIRT031312 LMNB2 lamin B2 1 1
MIRT031313 FAM127B family with sequence similarity 127, member B 1 1
MIRT031314 PAFAH1B1 platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa) 1 1
MIRT031315 RAB23 RAB23, member RAS oncogene family 1 1
MIRT031316 MID1 midline 1 (Opitz/BBB syndrome) 1 1
MIRT031317 ZNF711 zinc finger protein 711 1 1
MIRT031318 MOB4 MOB1, Mps One Binder kinase activator-like 3 (yeast) 1 1
MIRT031319 SON SON DNA binding protein 1 1
MIRT031320 SLC36A1 solute carrier family 36 (proton/amino acid symporter), member 1 1 1
MIRT031321 ACTL6A actin-like 6A 1 1
MIRT031322 CKAP5 cytoskeleton associated protein 5 1 1
MIRT031323 PSMB5 proteasome (prosome, macropain) subunit, beta type, 5 1 1
MIRT031324 TMUB2 transmembrane and ubiquitin-like domain containing 2 1 1
MIRT031325 NCOA6 nuclear receptor coactivator 6 1 1
MIRT031326 POGK pogo transposable element with KRAB domain 1 2
MIRT031327 KIAA0907 KIAA0907 1 1
MIRT031328 DCUN1D3 DCN1, defective in cullin neddylation 1, domain containing 3 (S. cerevisiae) 1 1
MIRT031329 GABPA GA binding protein transcription factor, alpha subunit 60kDa 1 1
MIRT031330 PCNP PEST proteolytic signal containing nuclear protein 1 1
MIRT031331 HNRNPR heterogeneous nuclear ribonucleoprotein R 1 1
MIRT031332 RASSF3 Ras association (RalGDS/AF-6) domain family member 3 1 1
MIRT031333 HOXA9 homeobox A9 1 1
MIRT031334 ZNF33A zinc finger protein 33A 1 1
MIRT031335 ACIN1 apoptotic chromatin condensation inducer 1 1 1
MIRT031336 FEM1C fem-1 homolog c (C. elegans) 1 1
MIRT031337 ZNF708 zinc finger protein 708 1 1
MIRT031338 EZR ezrin 1 1
MIRT031339 SERTAD3 SERTA domain containing 3 1 1
MIRT031340 FAM120C family with sequence similarity 120C 1 1
MIRT031341 PPP1CB protein phosphatase 1, catalytic subunit, beta isozyme 1 1
MIRT031342 UBE2G1 ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast) 1 1
MIRT031343 UBL5 ubiquitin-like 5 1 1
MIRT031344 UBTD2 ubiquitin domain containing 2 1 1
MIRT031345 SUPT7L suppressor of Ty 7 (S. cerevisiae)-like 1 1
MIRT031346 AP3S2 adaptor-related protein complex 3, sigma 2 subunit 1 1
MIRT031347 SYPL1 synaptophysin-like 1 1 1
MIRT031348 CA12 carbonic anhydrase XII 1 1
MIRT031349 TNRC6B trinucleotide repeat containing 6B 1 1
MIRT031350 CTDSPL CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like 1 1
MIRT031351 RAB5A RAB5A, member RAS oncogene family 1 1
MIRT031352 PARD6B par-6 partitioning defective 6 homolog beta (C. elegans) 1 1
MIRT031353 HMGCS1 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) 1 1
MIRT031354 XYLT2 xylosyltransferase II 1 1
MIRT031355 DAAM2 dishevelled associated activator of morphogenesis 2 1 1
MIRT031356 PDE4D phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) 1 1
MIRT031357 CA13 carbonic anhydrase XIII 1 1
MIRT031358 GIGYF1 GRB10 interacting GYF protein 1 1 2
MIRT031359 MAP7D1 MAP7 domain containing 1 1 1
MIRT031360 CREBL2 cAMP responsive element binding protein-like 2 1 1
MIRT050649 ETS2 v-ets erythroblastosis virus E26 oncogene homolog 2 (avian) 1 1
MIRT050650 ZNF703 zinc finger protein 703 1 1
MIRT050651 ZC3H18 zinc finger CCCH-type containing 18 1 1
MIRT050652 RBPJ recombination signal binding protein for immunoglobulin kappa J region 1 1
MIRT050653 DDAH1 dimethylarginine dimethylaminohydrolase 1 1 1
MIRT050654 ZSWIM6 zinc finger, SWIM-type containing 6 1 1
MIRT050655 MXD3 MAX dimerization protein 3 1 1
MIRT050656 IRS4 insulin receptor substrate 4 1 1
MIRT050657 MSL3 male-specific lethal 3 homolog (Drosophila) 1 1
MIRT050658 ATP1A1 ATPase, Na+/K+ transporting, alpha 1 polypeptide 1 1
MIRT050659 AFF4 AF4/FMR2 family, member 4 1 1
MIRT050660 EEF2 eukaryotic translation elongation factor 2 1 1
MIRT050661 PELP1 proline, glutamate and leucine rich protein 1 1 1
MIRT050662 DDX5 DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 1 1
MIRT050663 PON2 paraoxonase 2 1 1
MIRT050664 TLE3 transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) 1 1
MIRT050665 YBX3 cold shock domain protein A 1 1
MIRT050666 RPL9 ribosomal protein L9 1 1
MIRT050667 STX12 syntaxin 12 1 1
MIRT050668 RICTOR RPTOR independent companion of MTOR, complex 2 1 1
MIRT050669 LRIG3 leucine-rich repeats and immunoglobulin-like domains 3 1 1
MIRT050670 CIZ1 CDKN1A interacting zinc finger protein 1 1 1
MIRT050671 GCH1 GTP cyclohydrolase 1 1 1
MIRT050672 GNB2L1 guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 1 1
MIRT050673 DCAF7 DDB1 and CUL4 associated factor 7 1 1
MIRT050674 TNPO1 transportin 1 1 1
MIRT050675 ZMYND8 zinc finger, MYND-type containing 8 1 1
MIRT050676 ZFP36L2 zinc finger protein 36, C3H type-like 2 1 1
MIRT050677 MCM5 minichromosome maintenance complex component 5 1 1
MIRT050678 ERRFI1 ERBB receptor feedback inhibitor 1 1 1
MIRT050679 EEF1A1 eukaryotic translation elongation factor 1 alpha 1 1 1
MIRT050680 IGHMBP2 immunoglobulin mu binding protein 2 1 1
MIRT050681 RPL7A ribosomal protein L7a 1 1
MIRT050682 TUT1 terminal uridylyl transferase 1, U6 snRNA-specific 1 1
MIRT050683 TCP1 t-complex 1 1 1
MIRT050684 DIAPH1 diaphanous homolog 1 (Drosophila) 1 1
MIRT050685 CUL4A cullin 4A 1 1
MIRT050686 ZNF770 zinc finger protein 770 1 1
MIRT050687 COX7B cytochrome c oxidase subunit VIIb 1 1
MIRT050688 MATR3 matrin 3 1 1
MIRT050689 CCT6A chaperonin containing TCP1, subunit 6A (zeta 1) 1 1
MIRT050690 PPIA peptidylprolyl isomerase A (cyclophilin A) 1 1
MIRT050691 UBQLN4 ubiquilin 4 1 1
MIRT050692 CANX calnexin 1 1
MIRT050693 NBEA neurobeachin 1 1
MIRT050694 FAM104A family with sequence similarity 104, member A 1 1
MIRT050695 PI4KB phosphatidylinositol 4-kinase, catalytic, beta 1 1
MIRT050696 AHRR aryl-hydrocarbon receptor repressor 1 1
MIRT050697 MDC1 mediator of DNA-damage checkpoint 1 1 1
MIRT050698 SASS6 spindle assembly 6 homolog (C. elegans) 1 1
MIRT050699 RNF4 ring finger protein 4 1 1
MIRT050700 ACAP3 ArfGAP with coiled-coil, ankyrin repeat and PH domains 3 1 1
MIRT050701 GDAP2 ganglioside induced differentiation associated protein 2 1 1
MIRT050702 CXorf40B chromosome X open reading frame 40B 1 1
MIRT050703 EIF4EBP2 eukaryotic translation initiation factor 4E binding protein 2 1 1
MIRT050704 DCTN2 dynactin 2 (p50) 1 1
MIRT050705 RABAC1 Rab acceptor 1 (prenylated) 1 1
MIRT050706 ZMYM3 zinc finger, MYM-type 3 1 1
MIRT050707 LRRC41 leucine rich repeat containing 41 1 1
MIRT050708 CDC20 cell division cycle 20 homolog (S. cerevisiae) 1 1
MIRT050709 SERBP1 SERPINE1 mRNA binding protein 1 1 1
MIRT050710 UBC ubiquitin C 1 1
MIRT050711 PNPO pyridoxamine 5'-phosphate oxidase 1 1
MIRT050712 TRAPPC1 trafficking protein particle complex 1 1 1
MIRT050713 LARGE like-glycosyltransferase 1 1
MIRT050714 FAM173B family with sequence similarity 173, member B 1 1
MIRT050715 BVES blood vessel epicardial substance 1 1
MIRT050716 PDIK1L PDLIM1 interacting kinase 1 like 1 1
MIRT050717 WAC WW domain containing adaptor with coiled-coil 1 1
MIRT050718 SMCHD1 structural maintenance of chromosomes flexible hinge domain containing 1 1 1
MIRT050719 SRSF6 splicing factor, arginine/serine-rich 6 1 1
MIRT050720 PAPSS2 3'-phosphoadenosine 5'-phosphosulfate synthase 2 1 1
MIRT050721 ARVCF armadillo repeat gene deleted in velocardiofacial syndrome 1 1
MIRT050722 PCNA proliferating cell nuclear antigen 1 1
MIRT050723 SEC24B SEC24 family, member B (S. cerevisiae) 1 1
MIRT050724 HERC1 hect (homologous to the E6-AP (UBE3A) carboxyl terminus) domain and RCC1 (CHC1)-like domain (RLD) 1 1 1
MIRT050725 PKMYT1 protein kinase, membrane associated tyrosine/threonine 1 1 1
MIRT050726 KIAA0100 KIAA0100 1 1
MIRT050727 GLRX5 glutaredoxin 5 1 1
MIRT050728 ABR active BCR-related gene 1 1
MIRT050729 WWTR1 WW domain containing transcription regulator 1 1 1
MIRT050730 IRGQ immunity-related GTPase family, Q 1 1
MIRT050731 TCEA2 transcription elongation factor A (SII), 2 1 1
MIRT050732 CDCA5 cell division cycle associated 5 1 1
MIRT050733 ETFA electron-transfer-flavoprotein, alpha polypeptide 1 1
MIRT050734 VPS37A vacuolar protein sorting 37 homolog A (S. cerevisiae) 1 1
MIRT050735 RNF157 ring finger protein 157 1 1
MIRT050736 TXNDC15 thioredoxin domain containing 15 1 1
MIRT050737 ORAI3 ORAI calcium release-activated calcium modulator 3 1 1
MIRT050738 B4GALT1 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 1 1
MIRT050739 ADAM15 ADAM metallopeptidase domain 15 1 1