1. Statistics of miRNA-target interactions in miRTarBase
2. Major improvements
| Species | No. of miRNA-target interactions (MTIs) | No. of miRNAs | No. of target genes | No. of articles | No. of MTIs are validated by | ||||
|---|---|---|---|---|---|---|---|---|---|
| Western blot | Luciferase assay | pSILAC | Microarray | NGS | |||||
| Total | 370,211 | 5,015 | 21,001 | 5,356 | 4,580 | 6,695 | 502 | 13,587 | 348,007 |
| Human | 324,219 | 2,619 | 12,738 | 4,264 | 3,527 | 5,081 | 500 | 12,592 | 304,988 |
| Mouse | 41,588 | 1,885 | 7,146 | 669 | 744 | 1,038 | 2 | 753 | 39,818 |
| Rat | 488 | 150 | 247 | 198 | 212 | 220 | 0 | 204 | 2 |
| Chicken | 55 | 31 | 34 | 27 | 11 | 45 | 0 | 3 | 0 |
| Cattle | 242 | 21 | 111 | 10 | 4 | 8 | 0 | 1 | 0 |
| Sheep | 3 | 1 | 3 | 4 | 0 | 1 | 0 | 0 | 0 |
| Japanese medaka | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 |
| Zebrafish | 135 | 39 | 98 | 52 | 52 | 103 | 0 | 7 | 0 |
| Fruit fly | 147 | 45 | 86 | 47 | 19 | 141 | 0 | 13 | 0 |
| Silkworm | 3 | 3 | 2 | 2 | 0 | 3 | 0 | 0 | 0 |
| African clawed frog | 4 | 2 | 4 | 5 | 0 | 1 | 0 | 0 | 0 |
| Nematode | 3,221 | 154 | 454 | 32 | 2 | 41 | 0 | 1 | 3,178 |
| Western clawed frog | 2 | 1 | 2 | 2 | 0 | 1 | 0 | 0 | 0 |
| Rice | 1 | 1 | 1 | 7 | 0 | 0 | 0 | 0 | 0 |
| Thale cress | 94 | 40 | 68 | 19 | 7 | 3 | 0 | 13 | 21 |
| Epstein-Barr virus | 1 | 11 | 1 | 7 | 1 | 1 | 0 | 0 | 0 |
| HHV-8 | 1 | 8 | 1 | 3 | 0 | 1 | 0 | 0 | 0 |
| Human cytomegalovirus | 2 | 3 | 2 | 6 | 1 | 2 | 0 | 0 | 0 |
| VSV | 4 | 0 | 2 | 1 | 0 | 4 | 0 | 0 | 0 |
2. Major improvements